Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, <i>Cryptomeria japonica</i> (Thunb. ex L.f.) D. Don)
This paper presents the results of several experiments identifying basal salts (BS) contained in maturation medium, polyethylene glycol (PEG) concentration, abscisic acid (ABA) concentration, additional supplementation with potassium chloride (KCl), amino acid (AA) concentration, and proliferation c...
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MDPI AG
2021-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/5/874 |
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| author | Tsuyoshi E. Maruyama Saneyoshi Ueno Hideki Mori Takumi Kaneeda Yoshinari Moriguchi |
| author_facet | Tsuyoshi E. Maruyama Saneyoshi Ueno Hideki Mori Takumi Kaneeda Yoshinari Moriguchi |
| author_sort | Tsuyoshi E. Maruyama |
| collection | DOAJ |
| description | This paper presents the results of several experiments identifying basal salts (BS) contained in maturation medium, polyethylene glycol (PEG) concentration, abscisic acid (ABA) concentration, additional supplementation with potassium chloride (KCl), amino acid (AA) concentration, and proliferation culture medium (PCM) as the main culture factors affecting somatic embryo maturation in sugi (Japanese cedar, <i>Cryptomeria japonica,</i> Cupressaceae). Highly efficient embryo maturation was achieved when embryogenic cell lines (ECLs) were cultured on media supplemented with a combination of PEG, ABA, and AAs. More than 1000 embryos per gram of fresh weight (FW) can be produced on EM maturation medium supplemented with 175 g L<sup>−1</sup> PEG, 100 µM ABA, 2 g L<sup>−1</sup> glutamine, 1 g L<sup>−1</sup> asparagine, and 0.5 g L<sup>−1</sup> arginine. |
| first_indexed | 2024-03-10T11:56:36Z |
| format | Article |
| id | doaj.art-07a8bec4c92d449f89c3f43705a9719f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T11:56:36Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-07a8bec4c92d449f89c3f43705a9719f2023-11-21T17:15:37ZengMDPI AGPlants2223-77472021-04-0110587410.3390/plants10050874Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, <i>Cryptomeria japonica</i> (Thunb. ex L.f.) D. Don)Tsuyoshi E. Maruyama0Saneyoshi Ueno1Hideki Mori2Takumi Kaneeda3Yoshinari Moriguchi4Department of Research Planning and Coordination, Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba 305-8687, JapanDepartment of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba 305-8687, JapanDepartment of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba 305-8687, JapanGraduate School of Science and Technology, Niigata University, Ikarashi 8050, Niigata 950-2181, JapanGraduate School of Science and Technology, Niigata University, Ikarashi 8050, Niigata 950-2181, JapanThis paper presents the results of several experiments identifying basal salts (BS) contained in maturation medium, polyethylene glycol (PEG) concentration, abscisic acid (ABA) concentration, additional supplementation with potassium chloride (KCl), amino acid (AA) concentration, and proliferation culture medium (PCM) as the main culture factors affecting somatic embryo maturation in sugi (Japanese cedar, <i>Cryptomeria japonica,</i> Cupressaceae). Highly efficient embryo maturation was achieved when embryogenic cell lines (ECLs) were cultured on media supplemented with a combination of PEG, ABA, and AAs. More than 1000 embryos per gram of fresh weight (FW) can be produced on EM maturation medium supplemented with 175 g L<sup>−1</sup> PEG, 100 µM ABA, 2 g L<sup>−1</sup> glutamine, 1 g L<sup>−1</sup> asparagine, and 0.5 g L<sup>−1</sup> arginine.https://www.mdpi.com/2223-7747/10/5/874ABAamino acidcotyledonary embryoEM mediumKClPEG |
| spellingShingle | Tsuyoshi E. Maruyama Saneyoshi Ueno Hideki Mori Takumi Kaneeda Yoshinari Moriguchi Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, <i>Cryptomeria japonica</i> (Thunb. ex L.f.) D. Don) Plants ABA amino acid cotyledonary embryo EM medium KCl PEG |
| title | Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, <i>Cryptomeria japonica</i> (Thunb. ex L.f.) D. Don) |
| title_full | Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, <i>Cryptomeria japonica</i> (Thunb. ex L.f.) D. Don) |
| title_fullStr | Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, <i>Cryptomeria japonica</i> (Thunb. ex L.f.) D. Don) |
| title_full_unstemmed | Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, <i>Cryptomeria japonica</i> (Thunb. ex L.f.) D. Don) |
| title_short | Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, <i>Cryptomeria japonica</i> (Thunb. ex L.f.) D. Don) |
| title_sort | factors influencing somatic embryo maturation in sugi japanese cedar i cryptomeria japonica i thunb ex l f d don |
| topic | ABA amino acid cotyledonary embryo EM medium KCl PEG |
| url | https://www.mdpi.com/2223-7747/10/5/874 |
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