Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine
Nine major cell populations among 46,716 cells were identified in mouse intestinal ischemia‒reperfusion (II/R) injury by single-cell RNA sequencing. For enterocyte cells, 11 subclusters were found, in which enterocyte cluster 1 (EC1), enterocyte cluster 3 (EC3), and enterocyte cluster 8 (EC8) were n...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177923000059 |
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| author | Lianhong Yin Meng Gao Lina Xu Yan Qi Lan Han Jinyong Peng |
| author_facet | Lianhong Yin Meng Gao Lina Xu Yan Qi Lan Han Jinyong Peng |
| author_sort | Lianhong Yin |
| collection | DOAJ |
| description | Nine major cell populations among 46,716 cells were identified in mouse intestinal ischemia‒reperfusion (II/R) injury by single-cell RNA sequencing. For enterocyte cells, 11 subclusters were found, in which enterocyte cluster 1 (EC1), enterocyte cluster 3 (EC3), and enterocyte cluster 8 (EC8) were newly discovered cells in ischemia 45 min/reperfusion 720 min (I 45 min/R 720 min) group. EC1 and EC3 played roles in digestion and absorption, and EC8 played a role in cell junctions. For TA cells, after ischemia 45 min/reperfusion 90 min (I 45 min/R 90 min), many TA cells at the stage of proliferation were identified. For Paneth cells, Paneth cluster 3 was observed in the resting state of normal jejunum. After I 45 min/R 90 min, three new subsets were found, in which Paneth cluster 1 had good antigen presentation activity. The main functions of goblet cells were to synthesize and secrete mucus, and a novel subcluster (goblet cluster 5) with highly proliferative ability was discovered in I 45 min/R 90 min group. As a major part of immune system, the changes in T cells with important roles were clarified. Notably, enterocyte cells secreted Guca2b to interact with Gucy2c receptor on the membranes of stem cells, TA cells, Paneth cells, and goblet cells to elicit intercellular communication. One marker known as glutathione S-transferase mu 3 (GSTM3) affected intestinal mucosal barrier function by adjusting mitogen-activated protein kinases (MAPK) signaling during II/R injury. The data on the heterogeneity of intestinal cells, cellular communication and the mechanism of GSTM3 provide a cellular basis for treating II/R injury. |
| first_indexed | 2024-03-12T17:11:55Z |
| format | Article |
| id | doaj.art-07aa2818005c43ba85cfbc7e75e354d3 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-03-12T17:11:55Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-07aa2818005c43ba85cfbc7e75e354d32023-08-06T04:37:05ZengElsevierJournal of Pharmaceutical Analysis2095-17792023-07-01137760775Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestineLianhong Yin0Meng Gao1Lina Xu2Yan Qi3Lan Han4Jinyong Peng5College of Pharmacy, Dalian Medical University, Dalian, Liaoning, 116044, ChinaCollege of Pharmacy, Dalian Medical University, Dalian, Liaoning, 116044, ChinaCollege of Pharmacy, Dalian Medical University, Dalian, Liaoning, 116044, ChinaCollege of Pharmacy, Dalian Medical University, Dalian, Liaoning, 116044, ChinaSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China; Corresponding author.College of Pharmacy, Dalian Medical University, Dalian, Liaoning, 116044, China; Corresponding author.Nine major cell populations among 46,716 cells were identified in mouse intestinal ischemia‒reperfusion (II/R) injury by single-cell RNA sequencing. For enterocyte cells, 11 subclusters were found, in which enterocyte cluster 1 (EC1), enterocyte cluster 3 (EC3), and enterocyte cluster 8 (EC8) were newly discovered cells in ischemia 45 min/reperfusion 720 min (I 45 min/R 720 min) group. EC1 and EC3 played roles in digestion and absorption, and EC8 played a role in cell junctions. For TA cells, after ischemia 45 min/reperfusion 90 min (I 45 min/R 90 min), many TA cells at the stage of proliferation were identified. For Paneth cells, Paneth cluster 3 was observed in the resting state of normal jejunum. After I 45 min/R 90 min, three new subsets were found, in which Paneth cluster 1 had good antigen presentation activity. The main functions of goblet cells were to synthesize and secrete mucus, and a novel subcluster (goblet cluster 5) with highly proliferative ability was discovered in I 45 min/R 90 min group. As a major part of immune system, the changes in T cells with important roles were clarified. Notably, enterocyte cells secreted Guca2b to interact with Gucy2c receptor on the membranes of stem cells, TA cells, Paneth cells, and goblet cells to elicit intercellular communication. One marker known as glutathione S-transferase mu 3 (GSTM3) affected intestinal mucosal barrier function by adjusting mitogen-activated protein kinases (MAPK) signaling during II/R injury. The data on the heterogeneity of intestinal cells, cellular communication and the mechanism of GSTM3 provide a cellular basis for treating II/R injury.http://www.sciencedirect.com/science/article/pii/S2095177923000059Single-cell RNA-sequencingIntestinal cellsCell heterogeneityEnterocyte cellsIntercellular communication |
| spellingShingle | Lianhong Yin Meng Gao Lina Xu Yan Qi Lan Han Jinyong Peng Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine Journal of Pharmaceutical Analysis Single-cell RNA-sequencing Intestinal cells Cell heterogeneity Enterocyte cells Intercellular communication |
| title | Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine |
| title_full | Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine |
| title_fullStr | Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine |
| title_full_unstemmed | Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine |
| title_short | Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine |
| title_sort | single cell analysis of cellular heterogeneity and interactions in the ischemia reperfusion injured mouse intestine |
| topic | Single-cell RNA-sequencing Intestinal cells Cell heterogeneity Enterocyte cells Intercellular communication |
| url | http://www.sciencedirect.com/science/article/pii/S2095177923000059 |
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