Optimized strategy for real-time qPCR detection of Onchocerca volvulus DNA in pooled Simulium sp. blackfly vectors.

Optimized strategy for real-time qPCR detection of Onchocerca volvulus DNA in pooled Simulium sp. blackfly vectors.

<h4>Background</h4>Onchocerca volvulus is a filarial parasite that is a major cause of dermatitis and blindness in endemic regions primarily in sub-Saharan Africa. Widespread efforts to control the disease caused by O. volvulus infection (onchocerciasis) began in 1974 and in recent years...

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Main Authors: Mary Doherty, Jessica R Grant, Nils Pilotte, Sasisekhar Bennuru, Kerstin Fischer, Peter U Fischer, Sara Lustigman, Thomas B Nutman, Kenneth Pfarr, Achim Hoerauf, Thomas R Unnasch, Hassan K Hassan, Samuel Wanji, Patrick J Lammie, Eric Ottesen, Charles Mackenzie, Steven A Williams
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2023-12-01
Series:PLoS Neglected Tropical Diseases
Online Access:https://doi.org/10.1371/journal.pntd.0011815

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