Integrated transcriptomic and proteomic analyses of two sugarcane (Saccharum officinarum Linn.) varieties differing in their lodging tolerance
Abstract Background Lodging seriously affects sugarcane stem growth and sugar accumulation, reduces sugarcane yield and sucrose content, and impedes mechanization. However, the molecular mechanisms underlying sugarcane lodging tolerance remain unclear. In this study, comprehensive transcriptomic and...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04622-z |
| _version_ | 1797415587389898752 |
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| author | Xiang Li Yijie Li Ailin Wei Zeping Wang Hairong Huang Quyan Huang Litao Yang Yijing Gao Guanghu Zhu Qihuai Liu Yangrui Li Shaolong Wei Debin Wei |
| author_facet | Xiang Li Yijie Li Ailin Wei Zeping Wang Hairong Huang Quyan Huang Litao Yang Yijing Gao Guanghu Zhu Qihuai Liu Yangrui Li Shaolong Wei Debin Wei |
| author_sort | Xiang Li |
| collection | DOAJ |
| description | Abstract Background Lodging seriously affects sugarcane stem growth and sugar accumulation, reduces sugarcane yield and sucrose content, and impedes mechanization. However, the molecular mechanisms underlying sugarcane lodging tolerance remain unclear. In this study, comprehensive transcriptomic and proteomic analyses were performed to explore the differential genetic regulatory mechanisms between upright (GT42) and lodged (GF98-296) sugarcane varieties. Results The stain test showed that GT42 had more lignin and vascular bundles in the stem than GF98-296. The gene expression analysis revealed that the genes that were differentially expressed between the two varieties were mainly involved in the phenylpropanoid pathway at the growth stage. The protein expression analysis indicated that the proteins that were differentially expressed between the two varieties were related to the synthesis of secondary metabolites, the process of endocytosis, and the formation of aminoacyl-tRNA. Time-series analysis revealed variations in differential gene expression patterns between the two varieties, whereas significant protein expression trends in the two varieties were largely consistent, except for one profile. The expression of CYP84A, 4CL, and CAD from the key phenylpropanoid biosynthetic pathway was enhanced in GT42 at stage 2 but suppressed in GF98-296 at the growth stage. Furthermore, the expression of SDT1 in the nicotinate and nicotinamide metabolism was enhanced in GT42 cells but suppressed in GF98-296 cells at the growth stage. Conclusion Our findings provide reference data for mining lodging tolerance-related genes that are expected to facilitate the selective breeding of sugarcane varieties with excellent lodging tolerance. |
| first_indexed | 2024-03-09T05:50:53Z |
| format | Article |
| id | doaj.art-07abe14593f94fd4ab5878be0a0c6311 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-09T05:50:53Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-07abe14593f94fd4ab5878be0a0c63112023-12-03T12:17:13ZengBMCBMC Plant Biology1471-22292023-11-0123111510.1186/s12870-023-04622-zIntegrated transcriptomic and proteomic analyses of two sugarcane (Saccharum officinarum Linn.) varieties differing in their lodging toleranceXiang Li0Yijie Li1Ailin Wei2Zeping Wang3Hairong Huang4Quyan Huang5Litao Yang6Yijing Gao7Guanghu Zhu8Qihuai Liu9Yangrui Li10Shaolong Wei11Debin Wei12Guangxi Subtropical Crops Research InstituteGuangxi Academy of Agricultural SciencesBaise Institue of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesCenter for Applied Mathematics of Guangxi (GUET)Center for Applied Mathematics of Guangxi (GUET)Guangxi Academy of Agricultural SciencesGuangxi Subtropical Crops Research InstituteBaise Institue of Agricultural SciencesAbstract Background Lodging seriously affects sugarcane stem growth and sugar accumulation, reduces sugarcane yield and sucrose content, and impedes mechanization. However, the molecular mechanisms underlying sugarcane lodging tolerance remain unclear. In this study, comprehensive transcriptomic and proteomic analyses were performed to explore the differential genetic regulatory mechanisms between upright (GT42) and lodged (GF98-296) sugarcane varieties. Results The stain test showed that GT42 had more lignin and vascular bundles in the stem than GF98-296. The gene expression analysis revealed that the genes that were differentially expressed between the two varieties were mainly involved in the phenylpropanoid pathway at the growth stage. The protein expression analysis indicated that the proteins that were differentially expressed between the two varieties were related to the synthesis of secondary metabolites, the process of endocytosis, and the formation of aminoacyl-tRNA. Time-series analysis revealed variations in differential gene expression patterns between the two varieties, whereas significant protein expression trends in the two varieties were largely consistent, except for one profile. The expression of CYP84A, 4CL, and CAD from the key phenylpropanoid biosynthetic pathway was enhanced in GT42 at stage 2 but suppressed in GF98-296 at the growth stage. Furthermore, the expression of SDT1 in the nicotinate and nicotinamide metabolism was enhanced in GT42 cells but suppressed in GF98-296 cells at the growth stage. Conclusion Our findings provide reference data for mining lodging tolerance-related genes that are expected to facilitate the selective breeding of sugarcane varieties with excellent lodging tolerance.https://doi.org/10.1186/s12870-023-04622-zTranscriptomic and proteomic analysesLignificationLodging tolerance |
| spellingShingle | Xiang Li Yijie Li Ailin Wei Zeping Wang Hairong Huang Quyan Huang Litao Yang Yijing Gao Guanghu Zhu Qihuai Liu Yangrui Li Shaolong Wei Debin Wei Integrated transcriptomic and proteomic analyses of two sugarcane (Saccharum officinarum Linn.) varieties differing in their lodging tolerance BMC Plant Biology Transcriptomic and proteomic analyses Lignification Lodging tolerance |
| title | Integrated transcriptomic and proteomic analyses of two sugarcane (Saccharum officinarum Linn.) varieties differing in their lodging tolerance |
| title_full | Integrated transcriptomic and proteomic analyses of two sugarcane (Saccharum officinarum Linn.) varieties differing in their lodging tolerance |
| title_fullStr | Integrated transcriptomic and proteomic analyses of two sugarcane (Saccharum officinarum Linn.) varieties differing in their lodging tolerance |
| title_full_unstemmed | Integrated transcriptomic and proteomic analyses of two sugarcane (Saccharum officinarum Linn.) varieties differing in their lodging tolerance |
| title_short | Integrated transcriptomic and proteomic analyses of two sugarcane (Saccharum officinarum Linn.) varieties differing in their lodging tolerance |
| title_sort | integrated transcriptomic and proteomic analyses of two sugarcane saccharum officinarum linn varieties differing in their lodging tolerance |
| topic | Transcriptomic and proteomic analyses Lignification Lodging tolerance |
| url | https://doi.org/10.1186/s12870-023-04622-z |
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