Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neurons
The nutritional environment during development periods induces metabolic programming, leading to metabolic disorders and detrimental influences on hum an reproductive health. This study aimed to determine the long-term adverse effect of in trauterine malnutrition on the reproductive center kisspepti...
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Bioscientifica
2022-12-01
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| Series: | Endocrine Connections |
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| Online Access: | https://ec.bioscientifica.com/view/journals/ec/12/1/EC-22-0307.xml |
| _version_ | 1797980198179700736 |
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| author | Shiori Minabe Kinuyo Iwata Youki Watanabe Hirotaka Ishii Hitoshi Ozawa |
| author_facet | Shiori Minabe Kinuyo Iwata Youki Watanabe Hirotaka Ishii Hitoshi Ozawa |
| author_sort | Shiori Minabe |
| collection | DOAJ |
| description | The nutritional environment during development periods induces metabolic programming, leading to metabolic disorders and detrimental influences on hum an reproductive health. This study aimed to determine the long-term adverse effect of in trauterine malnutrition on the reproductive center kisspeptin-neurokinin B-dynorphin A (KNDy) neurons in the hypothalamic arcuate nucleus (ARC) of female offspring. Twelve p regnant rats were divided into ad-lib-fed (control, n = 6) and 50% undernutrition (UN, n = 6) groups. The UN group was restricted to 50% daily food intake of the control dams from gestation day 9 until term delivery. Differences between the two groups in ter ms of various maternal parameters, including body weight (BW), pregnancy duration, and litter size, as well as birth weight, puberty onset, estrous cyclicity, pulsatile luteinizing hormone (LH) secretion, and hypothalamic gene expression of offspring, were determined. Female offspring of UN dams exhibited low BW from birth to 3 weeks, whereas UN offsp ring showed signs of precocious puberty; hypothalamic Tac3 (a neurokinin B gene) expression was increased in prepubertal UN offspring, and the BW at the virginal opening was lower in UN offspring than that in the control group. Interestingly, the UN offspring showed significant decreases in the number of KNDy gene-expressing cells after 29 weeks of age, but the number of ARC kisspeptin-immunoreactive cells, pulsatile LH secretions, and estrous cyclicity were comparable between the groups. In conclusion, intrauterine unde rnutrition induced various changes in KNDy gene expression depending on the life s tage. Thus, intrauterine undernutrition affected hypothalamic developmental programming in female rats. |
| first_indexed | 2024-04-11T05:51:43Z |
| format | Article |
| id | doaj.art-07ae8e8fe5bb48798eefc1b2db90b950 |
| institution | Directory Open Access Journal |
| issn | 2049-3614 |
| language | English |
| last_indexed | 2024-04-11T05:51:43Z |
| publishDate | 2022-12-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-07ae8e8fe5bb48798eefc1b2db90b9502022-12-22T04:42:03ZengBioscientificaEndocrine Connections2049-36142022-12-01121111https://doi.org/10.1530/EC-22-0307Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neuronsShiori Minabe0Kinuyo Iwata1Youki Watanabe2Hirotaka Ishii3Hitoshi Ozawa4Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan; Division of Biomedical Information Analysis, Iwate Tohoku Medical Megabank Organization, Disaster Reconstruction Center, Iwate Medical University, Yahaba, JapanDepartment of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Tokyo, JapanDepartment of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Tokyo, JapanDepartment of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Tokyo, JapanDepartment of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan; Faculty of Health Science, Bukkyo University, Kyoto, Japan The nutritional environment during development periods induces metabolic programming, leading to metabolic disorders and detrimental influences on hum an reproductive health. This study aimed to determine the long-term adverse effect of in trauterine malnutrition on the reproductive center kisspeptin-neurokinin B-dynorphin A (KNDy) neurons in the hypothalamic arcuate nucleus (ARC) of female offspring. Twelve p regnant rats were divided into ad-lib-fed (control, n = 6) and 50% undernutrition (UN, n = 6) groups. The UN group was restricted to 50% daily food intake of the control dams from gestation day 9 until term delivery. Differences between the two groups in ter ms of various maternal parameters, including body weight (BW), pregnancy duration, and litter size, as well as birth weight, puberty onset, estrous cyclicity, pulsatile luteinizing hormone (LH) secretion, and hypothalamic gene expression of offspring, were determined. Female offspring of UN dams exhibited low BW from birth to 3 weeks, whereas UN offsp ring showed signs of precocious puberty; hypothalamic Tac3 (a neurokinin B gene) expression was increased in prepubertal UN offspring, and the BW at the virginal opening was lower in UN offspring than that in the control group. Interestingly, the UN offspring showed significant decreases in the number of KNDy gene-expressing cells after 29 weeks of age, but the number of ARC kisspeptin-immunoreactive cells, pulsatile LH secretions, and estrous cyclicity were comparable between the groups. In conclusion, intrauterine unde rnutrition induced various changes in KNDy gene expression depending on the life s tage. Thus, intrauterine undernutrition affected hypothalamic developmental programming in female rats.https://ec.bioscientifica.com/view/journals/ec/12/1/EC-22-0307.xmllow birth weightfetal programingkisspeptinprenatal undernutrition |
| spellingShingle | Shiori Minabe Kinuyo Iwata Youki Watanabe Hirotaka Ishii Hitoshi Ozawa Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neurons Endocrine Connections low birth weight fetal programing kisspeptin prenatal undernutrition |
| title | Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neurons |
| title_full | Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neurons |
| title_fullStr | Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neurons |
| title_full_unstemmed | Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neurons |
| title_short | Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neurons |
| title_sort | long term effects of prenatal undernutrition on female rat hypothalamic kndy neurons |
| topic | low birth weight fetal programing kisspeptin prenatal undernutrition |
| url | https://ec.bioscientifica.com/view/journals/ec/12/1/EC-22-0307.xml |
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