Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis
Abstract Dengue (DENV) and chikungunya (CHIKV) viruses are among the most preponderant arboviruses. Although primarily transmitted through the bite of Aedes aegypti mosquitoes, Aedes albopictus and Aedes malayensis are competent vectors and have an impact on arbovirus epidemiology. Here, to fill the...
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| Format: | Article |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38354-9 |
| _version_ | 1797778878786174976 |
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| author | Cassandra M. Modahl Avisha Chowdhury Dolyce H. W. Low Menchie C. Manuel Dorothée Missé R. Manjunatha Kini Ian H. Mendenhall Julien Pompon |
| author_facet | Cassandra M. Modahl Avisha Chowdhury Dolyce H. W. Low Menchie C. Manuel Dorothée Missé R. Manjunatha Kini Ian H. Mendenhall Julien Pompon |
| author_sort | Cassandra M. Modahl |
| collection | DOAJ |
| description | Abstract Dengue (DENV) and chikungunya (CHIKV) viruses are among the most preponderant arboviruses. Although primarily transmitted through the bite of Aedes aegypti mosquitoes, Aedes albopictus and Aedes malayensis are competent vectors and have an impact on arbovirus epidemiology. Here, to fill the gap in our understanding of the molecular interactions between secondary vectors and arboviruses, we used transcriptomics to profile the whole-genome responses of A. albopictus to CHIKV and of A. malayensis to CHIKV and DENV at 1 and 4 days post-infection (dpi) in midguts. In A. albopictus, 1793 and 339 genes were significantly regulated by CHIKV at 1 and 4 dpi, respectively. In A. malayensis, 943 and 222 genes upon CHIKV infection, and 74 and 69 genes upon DENV infection were significantly regulated at 1 and 4 dpi, respectively. We reported 81 genes that were consistently differentially regulated in all the CHIKV-infected conditions, identifying a CHIKV-induced signature. We identified expressed immune genes in both mosquito species, using a de novo assembled midgut transcriptome for A. malayensis, and described the immune architectures. We found the JNK pathway activated in all conditions, generalizing its antiviral function to Aedines. Our comprehensive study provides insight into arbovirus transmission by multiple Aedes vectors. |
| first_indexed | 2024-03-12T23:22:53Z |
| format | Article |
| id | doaj.art-07af2304b5d5410d9e85f51c3f0edb90 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T23:22:53Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-07af2304b5d5410d9e85f51c3f0edb902023-07-16T11:17:10ZengNature PortfolioScientific Reports2045-23222023-07-0113111310.1038/s41598-023-38354-9Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensisCassandra M. Modahl0Avisha Chowdhury1Dolyce H. W. Low2Menchie C. Manuel3Dorothée Missé4R. Manjunatha Kini5Ian H. Mendenhall6Julien Pompon7Department of Biological Sciences, National University of SingaporeDepartment of Biological Sciences, National University of SingaporeProgramme in Emerging Infectious Diseases, Duke-NUS Medical SchoolProgramme in Emerging Infectious Diseases, Duke-NUS Medical SchoolMIVEGEC, Univ. Montpellier, IRD, CNRSDepartment of Biological Sciences, National University of SingaporeProgramme in Emerging Infectious Diseases, Duke-NUS Medical SchoolProgramme in Emerging Infectious Diseases, Duke-NUS Medical SchoolAbstract Dengue (DENV) and chikungunya (CHIKV) viruses are among the most preponderant arboviruses. Although primarily transmitted through the bite of Aedes aegypti mosquitoes, Aedes albopictus and Aedes malayensis are competent vectors and have an impact on arbovirus epidemiology. Here, to fill the gap in our understanding of the molecular interactions between secondary vectors and arboviruses, we used transcriptomics to profile the whole-genome responses of A. albopictus to CHIKV and of A. malayensis to CHIKV and DENV at 1 and 4 days post-infection (dpi) in midguts. In A. albopictus, 1793 and 339 genes were significantly regulated by CHIKV at 1 and 4 dpi, respectively. In A. malayensis, 943 and 222 genes upon CHIKV infection, and 74 and 69 genes upon DENV infection were significantly regulated at 1 and 4 dpi, respectively. We reported 81 genes that were consistently differentially regulated in all the CHIKV-infected conditions, identifying a CHIKV-induced signature. We identified expressed immune genes in both mosquito species, using a de novo assembled midgut transcriptome for A. malayensis, and described the immune architectures. We found the JNK pathway activated in all conditions, generalizing its antiviral function to Aedines. Our comprehensive study provides insight into arbovirus transmission by multiple Aedes vectors.https://doi.org/10.1038/s41598-023-38354-9 |
| spellingShingle | Cassandra M. Modahl Avisha Chowdhury Dolyce H. W. Low Menchie C. Manuel Dorothée Missé R. Manjunatha Kini Ian H. Mendenhall Julien Pompon Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis Scientific Reports |
| title | Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis |
| title_full | Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis |
| title_fullStr | Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis |
| title_full_unstemmed | Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis |
| title_short | Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis |
| title_sort | midgut transcriptomic responses to dengue and chikungunya viruses in the vectors aedes albopictus and aedes malayensis |
| url | https://doi.org/10.1038/s41598-023-38354-9 |
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