Anti-biofilm and Antibacterial Activities of Silver Nanoparticles Synthesized by the Reducing Activity of Phytoconstituents Present in the Indian Medicinal Plants
Biofilm forming from a variety of microbial pathogens can pose a serious health hazard that is difficult to combat. Nanotechnology, however, represents a new approach to fighting and eradicating biofilm-forming microorganisms. In the present study, the sustainable synthesis and characterization of b...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01143/full |
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| author | Yugal Kishore Mohanta Kunal Biswas Santosh Kumar Jena Abeer Hashem Abeer Hashem Elsayed Fathi Abd_Allah Tapan Kumar Mohanta |
| author_facet | Yugal Kishore Mohanta Kunal Biswas Santosh Kumar Jena Abeer Hashem Abeer Hashem Elsayed Fathi Abd_Allah Tapan Kumar Mohanta |
| author_sort | Yugal Kishore Mohanta |
| collection | DOAJ |
| description | Biofilm forming from a variety of microbial pathogens can pose a serious health hazard that is difficult to combat. Nanotechnology, however, represents a new approach to fighting and eradicating biofilm-forming microorganisms. In the present study, the sustainable synthesis and characterization of biocompatible silver nanoparticles (AgNPs) from leaf extracts of Semecarpus anacardium, Glochidion lanceolarium, and Bridelia retusa was explored. Continuous synthesis was observed in a UV–vis spectroscopic analysis and the participating phytoconstituents, flavonoids, phenolic compounds, phytosterols, and glycosides, were characterized by Attenuated total reflectance-Fourier transform infrared spectroscopy. The size and surface charge of the particles were also measured by dynamic light scattering spectroscopy. Scanning electron microscopy study was employed to examine the morphology of the nanoparticles. The spectroscopic and microscopic study confirmed the successful synthesis of AgNPs by plant extracts acting as strong reducing agents. The synthesized AgNPs were screened for antibacterial and anti-biofilm activity against human pathogens Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. Results of the study demonstrate the potential of phyto-synthesized AgNPs to act as anti-biofilm agents and for other biomedical applications. |
| first_indexed | 2024-12-21T01:35:07Z |
| format | Article |
| id | doaj.art-07b0c032bd2e49b5a98edad7d6271ffa |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T01:35:07Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-07b0c032bd2e49b5a98edad7d6271ffa2022-12-21T19:20:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01143499780Anti-biofilm and Antibacterial Activities of Silver Nanoparticles Synthesized by the Reducing Activity of Phytoconstituents Present in the Indian Medicinal PlantsYugal Kishore Mohanta0Kunal Biswas1Santosh Kumar Jena2Abeer Hashem3Abeer Hashem4Elsayed Fathi Abd_Allah5Tapan Kumar Mohanta6Department of Botany, North Orissa University, Baripada, IndiaDepartment of Biotechnology, Maulana Abul Kalam Azad University of Technology, Haringhata, IndiaDepartment of Biotechnology, North Orissa University, Baripada, IndiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi ArabiaMycology and Plant Disease Survey Department, Plant Pathology Research Institute, Agriculture Research Center, Giza, EgyptPlant Production Department, College of Food & Agricultural Sciences, King Saud University, Riyadh, Saudi ArabiaNatural and Medical Sciences Research Center, University of Nizwa, Nizwa, OmanBiofilm forming from a variety of microbial pathogens can pose a serious health hazard that is difficult to combat. Nanotechnology, however, represents a new approach to fighting and eradicating biofilm-forming microorganisms. In the present study, the sustainable synthesis and characterization of biocompatible silver nanoparticles (AgNPs) from leaf extracts of Semecarpus anacardium, Glochidion lanceolarium, and Bridelia retusa was explored. Continuous synthesis was observed in a UV–vis spectroscopic analysis and the participating phytoconstituents, flavonoids, phenolic compounds, phytosterols, and glycosides, were characterized by Attenuated total reflectance-Fourier transform infrared spectroscopy. The size and surface charge of the particles were also measured by dynamic light scattering spectroscopy. Scanning electron microscopy study was employed to examine the morphology of the nanoparticles. The spectroscopic and microscopic study confirmed the successful synthesis of AgNPs by plant extracts acting as strong reducing agents. The synthesized AgNPs were screened for antibacterial and anti-biofilm activity against human pathogens Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. Results of the study demonstrate the potential of phyto-synthesized AgNPs to act as anti-biofilm agents and for other biomedical applications.https://www.frontiersin.org/article/10.3389/fmicb.2020.01143/fullphyto-synthesissilver nanoparticlesmedicinal plantsanti-bacterial activityanti-biofilm activity |
| spellingShingle | Yugal Kishore Mohanta Kunal Biswas Santosh Kumar Jena Abeer Hashem Abeer Hashem Elsayed Fathi Abd_Allah Tapan Kumar Mohanta Anti-biofilm and Antibacterial Activities of Silver Nanoparticles Synthesized by the Reducing Activity of Phytoconstituents Present in the Indian Medicinal Plants Frontiers in Microbiology phyto-synthesis silver nanoparticles medicinal plants anti-bacterial activity anti-biofilm activity |
| title | Anti-biofilm and Antibacterial Activities of Silver Nanoparticles Synthesized by the Reducing Activity of Phytoconstituents Present in the Indian Medicinal Plants |
| title_full | Anti-biofilm and Antibacterial Activities of Silver Nanoparticles Synthesized by the Reducing Activity of Phytoconstituents Present in the Indian Medicinal Plants |
| title_fullStr | Anti-biofilm and Antibacterial Activities of Silver Nanoparticles Synthesized by the Reducing Activity of Phytoconstituents Present in the Indian Medicinal Plants |
| title_full_unstemmed | Anti-biofilm and Antibacterial Activities of Silver Nanoparticles Synthesized by the Reducing Activity of Phytoconstituents Present in the Indian Medicinal Plants |
| title_short | Anti-biofilm and Antibacterial Activities of Silver Nanoparticles Synthesized by the Reducing Activity of Phytoconstituents Present in the Indian Medicinal Plants |
| title_sort | anti biofilm and antibacterial activities of silver nanoparticles synthesized by the reducing activity of phytoconstituents present in the indian medicinal plants |
| topic | phyto-synthesis silver nanoparticles medicinal plants anti-bacterial activity anti-biofilm activity |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01143/full |
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