Foliar Application of Nanoclay Promotes Potato (<i>Solanum tuberosum</i> L.) Growth and Induces Systemic Resistance against Potato Virus Y
Potato virus Y (PVY) is one of the most harmful phytopathogens. It causes big problems for potatoes and other important crops around the world. Nanoclays have been extensively studied for various biomedical applications. However, reports on their interactions with phytopathogens, particularly viral...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/10/2151 |
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| author | Dalia G. Aseel Ahmed Abdelkhalek Fatimah O. Alotibi Marwa A. Samy Abdulaziz A. Al-Askar Amr A. Arishi Elsayed E. Hafez |
| author_facet | Dalia G. Aseel Ahmed Abdelkhalek Fatimah O. Alotibi Marwa A. Samy Abdulaziz A. Al-Askar Amr A. Arishi Elsayed E. Hafez |
| author_sort | Dalia G. Aseel |
| collection | DOAJ |
| description | Potato virus Y (PVY) is one of the most harmful phytopathogens. It causes big problems for potatoes and other important crops around the world. Nanoclays have been extensively studied for various biomedical applications. However, reports on their interactions with phytopathogens, particularly viral infections, are still limited. In this study, the protective activity of Egyptian nanoclay (CE) and standard nanoclay (CS) against PVY was evaluated on potato (<i>Solanum tuberosum</i> L.) plants. Their physicochemical and morphological properties were examined with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and energy dispersive spectrometer (EDS). SEM and TEM analyses revealed that CE has a spherical and hexagonal structure ranging from 20 to 80 nm in size, while CS has boulder-like and tubular structures of about 320 nm in size. FTIR and EDS showed that both nanoclay types have different functional groups and contain many vital plant nutrients that are necessary for every stage and process of the plant, including development, productivity, and metabolism. Under greenhouse conditions, a 1% nanoclay foliar application enhanced potato growth, reduced disease symptoms, and reduced PVY accumulation levels compared with non-treated plants. Significant increases in levels of antioxidant enzymes (PPO and POX) and considerable decreases in oxidative stress markers (MDA and H<sub>2</sub>O<sub>2</sub>) were also reported. Moreover, a significant increase in the transcriptional levels of defense-related genes (<i>PAL-1</i>, <i>PR-5</i>, and <i>CHI-2</i>) was observed. All experiment and analysis results indicate that the CE type is more effective than the CS type against PVY infection. Based on these results, the foliar applications of nanoclay could be used to manage plant viral infections in a way that is both effective and environmentally friendly. To our knowledge, this is the first report of the antiviral activity of the foliar application of nanoclay against PVY infection. |
| first_indexed | 2024-03-09T19:23:35Z |
| format | Article |
| id | doaj.art-07b1866637754cd18530becde06a8b15 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T19:23:35Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-07b1866637754cd18530becde06a8b152023-11-24T03:08:20ZengMDPI AGViruses1999-49152022-09-011410215110.3390/v14102151Foliar Application of Nanoclay Promotes Potato (<i>Solanum tuberosum</i> L.) Growth and Induces Systemic Resistance against Potato Virus YDalia G. Aseel0Ahmed Abdelkhalek1Fatimah O. Alotibi2Marwa A. Samy3Abdulaziz A. Al-Askar4Amr A. Arishi5Elsayed E. Hafez6Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El Arab City 21934, EgyptPlant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El Arab City 21934, EgyptDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaPlant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El Arab City 21934, EgyptDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaSchool of Molecular Sciences, The University of Western Australia, Perth, WA 6009, AustraliaPlant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El Arab City 21934, EgyptPotato virus Y (PVY) is one of the most harmful phytopathogens. It causes big problems for potatoes and other important crops around the world. Nanoclays have been extensively studied for various biomedical applications. However, reports on their interactions with phytopathogens, particularly viral infections, are still limited. In this study, the protective activity of Egyptian nanoclay (CE) and standard nanoclay (CS) against PVY was evaluated on potato (<i>Solanum tuberosum</i> L.) plants. Their physicochemical and morphological properties were examined with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and energy dispersive spectrometer (EDS). SEM and TEM analyses revealed that CE has a spherical and hexagonal structure ranging from 20 to 80 nm in size, while CS has boulder-like and tubular structures of about 320 nm in size. FTIR and EDS showed that both nanoclay types have different functional groups and contain many vital plant nutrients that are necessary for every stage and process of the plant, including development, productivity, and metabolism. Under greenhouse conditions, a 1% nanoclay foliar application enhanced potato growth, reduced disease symptoms, and reduced PVY accumulation levels compared with non-treated plants. Significant increases in levels of antioxidant enzymes (PPO and POX) and considerable decreases in oxidative stress markers (MDA and H<sub>2</sub>O<sub>2</sub>) were also reported. Moreover, a significant increase in the transcriptional levels of defense-related genes (<i>PAL-1</i>, <i>PR-5</i>, and <i>CHI-2</i>) was observed. All experiment and analysis results indicate that the CE type is more effective than the CS type against PVY infection. Based on these results, the foliar applications of nanoclay could be used to manage plant viral infections in a way that is both effective and environmentally friendly. To our knowledge, this is the first report of the antiviral activity of the foliar application of nanoclay against PVY infection.https://www.mdpi.com/1999-4915/14/10/2151nanoclaypotatoPVYFTIRSEMTEM |
| spellingShingle | Dalia G. Aseel Ahmed Abdelkhalek Fatimah O. Alotibi Marwa A. Samy Abdulaziz A. Al-Askar Amr A. Arishi Elsayed E. Hafez Foliar Application of Nanoclay Promotes Potato (<i>Solanum tuberosum</i> L.) Growth and Induces Systemic Resistance against Potato Virus Y Viruses nanoclay potato PVY FTIR SEM TEM |
| title | Foliar Application of Nanoclay Promotes Potato (<i>Solanum tuberosum</i> L.) Growth and Induces Systemic Resistance against Potato Virus Y |
| title_full | Foliar Application of Nanoclay Promotes Potato (<i>Solanum tuberosum</i> L.) Growth and Induces Systemic Resistance against Potato Virus Y |
| title_fullStr | Foliar Application of Nanoclay Promotes Potato (<i>Solanum tuberosum</i> L.) Growth and Induces Systemic Resistance against Potato Virus Y |
| title_full_unstemmed | Foliar Application of Nanoclay Promotes Potato (<i>Solanum tuberosum</i> L.) Growth and Induces Systemic Resistance against Potato Virus Y |
| title_short | Foliar Application of Nanoclay Promotes Potato (<i>Solanum tuberosum</i> L.) Growth and Induces Systemic Resistance against Potato Virus Y |
| title_sort | foliar application of nanoclay promotes potato i solanum tuberosum i l growth and induces systemic resistance against potato virus y |
| topic | nanoclay potato PVY FTIR SEM TEM |
| url | https://www.mdpi.com/1999-4915/14/10/2151 |
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