Fabrication of protein-coated titanium dioxide nanoparticles for cellular uptake fluorescence imaging and treatment of colorectal cancer
Titanium dioxide (TiO _2 ) coated with different proteins has exhibited exceptional bio-compatibility, leading to various biomedical engineering applications. With the use of green and chemical reduction methods, we fabricated Titanium dioxide nanoparticles that were protein-coated. Bovine serum alb...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2021-01-01
|
| Series: | Materials Research Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2053-1591/ac3e25 |
| _version_ | 1797746714983006208 |
|---|---|
| author | Li Zhang Deping Wang Honglu Yu |
| author_facet | Li Zhang Deping Wang Honglu Yu |
| author_sort | Li Zhang |
| collection | DOAJ |
| description | Titanium dioxide (TiO _2 ) coated with different proteins has exhibited exceptional bio-compatibility, leading to various biomedical engineering applications. With the use of green and chemical reduction methods, we fabricated Titanium dioxide nanoparticles that were protein-coated. Bovine serum albumin (BSA), lysozyme proteins, zein, and collagen have been used to coat titanium dioxide-aryl nanoparticles of the form TiO _2 -NPs. However, in both cases, no catalysts or other stabilizing agents were used. These images of TiO _2 -NPs fabricated using the green method show high crystallinity. It is a malignant colorectal tumour with dysfunctional cellular processes that cause colorectal cancer cells. It is hoped that studies employing SW1417 cells would give mechanistic ideas on the specifics of the amplification in cancers. This was done by flow cytometry utilizing and laser confocal fluorescence microscopy (LCFM) on the SW1417 colorectal cell line. Of the protein-coated Titanium dioxide nanoparticles fabricated green methods, BSA@TiO _2 -NPs were the most readily absorbed. Of all TiO _2 -NPs, lysozyme@TiO _2 -NPs fabricated by the chemical reduction technique were the most effectively internalized by SW1417 cells out of TiO _2 -NPs types. However, TiO _2 -NPs fabricated by the green methodology were coated with zein and lysozyme and tiny. A hydrophobic covering is also on the two nanoparticles. There is a possibility that the variation in hydrophobicity and charge affected the internalization process. Colorectal diagnostic and therapeutic compounds might be synthesized from those coated nanoparticles that were effectively internalized. |
| first_indexed | 2024-03-12T15:40:49Z |
| format | Article |
| id | doaj.art-07b335fd0f94445ab2dfe16b6c3cf9b4 |
| institution | Directory Open Access Journal |
| issn | 2053-1591 |
| language | English |
| last_indexed | 2024-03-12T15:40:49Z |
| publishDate | 2021-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Materials Research Express |
| spelling | doaj.art-07b335fd0f94445ab2dfe16b6c3cf9b42023-08-09T15:58:27ZengIOP PublishingMaterials Research Express2053-15912021-01-0181212500810.1088/2053-1591/ac3e25Fabrication of protein-coated titanium dioxide nanoparticles for cellular uptake fluorescence imaging and treatment of colorectal cancerLi Zhang0Deping Wang1Honglu Yu2https://orcid.org/0000-0002-7003-1465Division of Gastroenterology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan-430077, People’s Republic of ChinaDivision of Emergency Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology , Wuhan-430077, People’s Republic of ChinaDivision of Gastroenterology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan-430077, People’s Republic of ChinaTitanium dioxide (TiO _2 ) coated with different proteins has exhibited exceptional bio-compatibility, leading to various biomedical engineering applications. With the use of green and chemical reduction methods, we fabricated Titanium dioxide nanoparticles that were protein-coated. Bovine serum albumin (BSA), lysozyme proteins, zein, and collagen have been used to coat titanium dioxide-aryl nanoparticles of the form TiO _2 -NPs. However, in both cases, no catalysts or other stabilizing agents were used. These images of TiO _2 -NPs fabricated using the green method show high crystallinity. It is a malignant colorectal tumour with dysfunctional cellular processes that cause colorectal cancer cells. It is hoped that studies employing SW1417 cells would give mechanistic ideas on the specifics of the amplification in cancers. This was done by flow cytometry utilizing and laser confocal fluorescence microscopy (LCFM) on the SW1417 colorectal cell line. Of the protein-coated Titanium dioxide nanoparticles fabricated green methods, BSA@TiO _2 -NPs were the most readily absorbed. Of all TiO _2 -NPs, lysozyme@TiO _2 -NPs fabricated by the chemical reduction technique were the most effectively internalized by SW1417 cells out of TiO _2 -NPs types. However, TiO _2 -NPs fabricated by the green methodology were coated with zein and lysozyme and tiny. A hydrophobic covering is also on the two nanoparticles. There is a possibility that the variation in hydrophobicity and charge affected the internalization process. Colorectal diagnostic and therapeutic compounds might be synthesized from those coated nanoparticles that were effectively internalized.https://doi.org/10.1088/2053-1591/ac3e25titanium dioxideproteincytotoxicitycellular uptakefluorescence imaging |
| spellingShingle | Li Zhang Deping Wang Honglu Yu Fabrication of protein-coated titanium dioxide nanoparticles for cellular uptake fluorescence imaging and treatment of colorectal cancer Materials Research Express titanium dioxide protein cytotoxicity cellular uptake fluorescence imaging |
| title | Fabrication of protein-coated titanium dioxide nanoparticles for cellular uptake fluorescence imaging and treatment of colorectal cancer |
| title_full | Fabrication of protein-coated titanium dioxide nanoparticles for cellular uptake fluorescence imaging and treatment of colorectal cancer |
| title_fullStr | Fabrication of protein-coated titanium dioxide nanoparticles for cellular uptake fluorescence imaging and treatment of colorectal cancer |
| title_full_unstemmed | Fabrication of protein-coated titanium dioxide nanoparticles for cellular uptake fluorescence imaging and treatment of colorectal cancer |
| title_short | Fabrication of protein-coated titanium dioxide nanoparticles for cellular uptake fluorescence imaging and treatment of colorectal cancer |
| title_sort | fabrication of protein coated titanium dioxide nanoparticles for cellular uptake fluorescence imaging and treatment of colorectal cancer |
| topic | titanium dioxide protein cytotoxicity cellular uptake fluorescence imaging |
| url | https://doi.org/10.1088/2053-1591/ac3e25 |
| work_keys_str_mv | AT lizhang fabricationofproteincoatedtitaniumdioxidenanoparticlesforcellularuptakefluorescenceimagingandtreatmentofcolorectalcancer AT depingwang fabricationofproteincoatedtitaniumdioxidenanoparticlesforcellularuptakefluorescenceimagingandtreatmentofcolorectalcancer AT hongluyu fabricationofproteincoatedtitaniumdioxidenanoparticlesforcellularuptakefluorescenceimagingandtreatmentofcolorectalcancer |