An In-vitro Assessment of Effect of Interaction Between Local Anaesthetic Solution with Sodium Hypochlorite on the Sealing Ability of Root Canal Obturation

Introduction: Endodontic treatment failures are caused by persistent or secondary infection due to inefficient cleaning or re-infection of the obturated root canal system because of coronal or apical leakage. Intrapulpal Injection (IPI) technique is the most commonly employed supplemental anaest...

Full description

Bibliographic Details
Main Authors: Urmila Banshbahadur Chauhan, Tipturmanjunath Mangala, Rushikesh Mahaparale, Adish Saraf, Sneha Mali, Sagar Pawar, Vincia Dsouza, Shubham Mandhane
Format: Article
Language:English
Published: JCDR Research and Publications Private Limited 2020-07-01
Series:Journal of Clinical and Diagnostic Research
Subjects:
Online Access:https://jcdr.net/articles/PDF/13835/43932_F(KM)_CE[Ra1]_(SHU)_PF1(AKA_KM)_GC(Suk_SL)_PN(SL).pdf
_version_ 1819054868495597568
author Urmila Banshbahadur Chauhan
Tipturmanjunath Mangala
Rushikesh Mahaparale
Adish Saraf
Sneha Mali
Sagar Pawar
Vincia Dsouza
Shubham Mandhane
author_facet Urmila Banshbahadur Chauhan
Tipturmanjunath Mangala
Rushikesh Mahaparale
Adish Saraf
Sneha Mali
Sagar Pawar
Vincia Dsouza
Shubham Mandhane
author_sort Urmila Banshbahadur Chauhan
collection DOAJ
description Introduction: Endodontic treatment failures are caused by persistent or secondary infection due to inefficient cleaning or re-infection of the obturated root canal system because of coronal or apical leakage. Intrapulpal Injection (IPI) technique is the most commonly employed supplemental anaesthetic procedure and NaOCl is considered as gold standard irrigating solution. Therefore, this study was designed to explore the action of precipitate form by interaction between LA and Sodium Hypochlorite (NaOCL) on sealing of root canal obturating material. Aim: To evaluate the effect of precipitate formed by interaction of local anaesthetic solution and NaOCl on the sealing ability of root canal obturation, using a push-out bond strength test. Materials and Methods: This was an in-vitro study from October 2019 to January 2020. In this study, forty single rooted premolars were selected and were randomly distributed equally into 4 groups with 10 specimens in each group, based on the test solutions employed. All the teeth were decoronated and the root length was standardised. Group I - irrigated with saline only, Group II- treated with 2% lidocaine hydrochloride with adrenaline 1:100,000 (2% LA) followed by 3% NaOCl, Group III - 2% LA followed by saline and 3% NaOCl, Group IV- 3% NaOCl followed by saline. All root canals were than instrumented using ProTaper Universal rotary system upto F3 and obturation was done using AH plus sealer and GuttaPercha. Sealing ability was evaluated using a micro push-out bond strength, with the help of a universal testing machine and data were analysed statistically. Results: One-way analysis of variance (ANOVA) showed that there is highly significant difference among the various groups (p <0.0001). Within the experimental groups, Group II showed minimum mean push out bond strength (16.39±2.40) as compared to Group III (21.83±1.25) and Group IV (22.50±2.12). Conclusion: Interaction of LA with NaOCl forms precipitate which blocks the dentinal tubules and reduces the mean push out bond strength. It is recommended to irrigate thoroughly with saline after intrapulpal injection (2%LA) before irrigating with NaOCl.
first_indexed 2024-12-21T12:58:28Z
format Article
id doaj.art-07b8cf0c435e4dbd9ffcaa74e6806d51
institution Directory Open Access Journal
issn 2249-782X
0973-709X
language English
last_indexed 2024-12-21T12:58:28Z
publishDate 2020-07-01
publisher JCDR Research and Publications Private Limited
record_format Article
series Journal of Clinical and Diagnostic Research
spelling doaj.art-07b8cf0c435e4dbd9ffcaa74e6806d512022-12-21T19:03:16ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2020-07-01147141710.7860/JCDR/2020/43932.13835An In-vitro Assessment of Effect of Interaction Between Local Anaesthetic Solution with Sodium Hypochlorite on the Sealing Ability of Root Canal ObturationUrmila Banshbahadur Chauhan0Tipturmanjunath Mangala1Rushikesh Mahaparale2Adish Saraf3Sneha Mali4Sagar Pawar5Vincia Dsouza6Shubham Mandhane7PG Student, Department of Conservative Dentistry and Endodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.PG Student, Department of Conservative Dentistry and Endodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.PG Student, Department of Conservative Dentistry and Endodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.PG Student, Department of Conservative Dentistry and Endodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.PG Student, Department of Conservative Dentistry and Endodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.PG Student, Department of Conservative Dentistry and Endodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.PG Student, Department of Conservative Dentistry and Endodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.PG Student, Department of Conservative Dentistry and Endodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.Introduction: Endodontic treatment failures are caused by persistent or secondary infection due to inefficient cleaning or re-infection of the obturated root canal system because of coronal or apical leakage. Intrapulpal Injection (IPI) technique is the most commonly employed supplemental anaesthetic procedure and NaOCl is considered as gold standard irrigating solution. Therefore, this study was designed to explore the action of precipitate form by interaction between LA and Sodium Hypochlorite (NaOCL) on sealing of root canal obturating material. Aim: To evaluate the effect of precipitate formed by interaction of local anaesthetic solution and NaOCl on the sealing ability of root canal obturation, using a push-out bond strength test. Materials and Methods: This was an in-vitro study from October 2019 to January 2020. In this study, forty single rooted premolars were selected and were randomly distributed equally into 4 groups with 10 specimens in each group, based on the test solutions employed. All the teeth were decoronated and the root length was standardised. Group I - irrigated with saline only, Group II- treated with 2% lidocaine hydrochloride with adrenaline 1:100,000 (2% LA) followed by 3% NaOCl, Group III - 2% LA followed by saline and 3% NaOCl, Group IV- 3% NaOCl followed by saline. All root canals were than instrumented using ProTaper Universal rotary system upto F3 and obturation was done using AH plus sealer and GuttaPercha. Sealing ability was evaluated using a micro push-out bond strength, with the help of a universal testing machine and data were analysed statistically. Results: One-way analysis of variance (ANOVA) showed that there is highly significant difference among the various groups (p <0.0001). Within the experimental groups, Group II showed minimum mean push out bond strength (16.39±2.40) as compared to Group III (21.83±1.25) and Group IV (22.50±2.12). Conclusion: Interaction of LA with NaOCl forms precipitate which blocks the dentinal tubules and reduces the mean push out bond strength. It is recommended to irrigate thoroughly with saline after intrapulpal injection (2%LA) before irrigating with NaOCl.https://jcdr.net/articles/PDF/13835/43932_F(KM)_CE[Ra1]_(SHU)_PF1(AKA_KM)_GC(Suk_SL)_PN(SL).pdfintrapulpal injectionprecipitationpush out bond strengthsealer
spellingShingle Urmila Banshbahadur Chauhan
Tipturmanjunath Mangala
Rushikesh Mahaparale
Adish Saraf
Sneha Mali
Sagar Pawar
Vincia Dsouza
Shubham Mandhane
An In-vitro Assessment of Effect of Interaction Between Local Anaesthetic Solution with Sodium Hypochlorite on the Sealing Ability of Root Canal Obturation
Journal of Clinical and Diagnostic Research
intrapulpal injection
precipitation
push out bond strength
sealer
title An In-vitro Assessment of Effect of Interaction Between Local Anaesthetic Solution with Sodium Hypochlorite on the Sealing Ability of Root Canal Obturation
title_full An In-vitro Assessment of Effect of Interaction Between Local Anaesthetic Solution with Sodium Hypochlorite on the Sealing Ability of Root Canal Obturation
title_fullStr An In-vitro Assessment of Effect of Interaction Between Local Anaesthetic Solution with Sodium Hypochlorite on the Sealing Ability of Root Canal Obturation
title_full_unstemmed An In-vitro Assessment of Effect of Interaction Between Local Anaesthetic Solution with Sodium Hypochlorite on the Sealing Ability of Root Canal Obturation
title_short An In-vitro Assessment of Effect of Interaction Between Local Anaesthetic Solution with Sodium Hypochlorite on the Sealing Ability of Root Canal Obturation
title_sort in vitro assessment of effect of interaction between local anaesthetic solution with sodium hypochlorite on the sealing ability of root canal obturation
topic intrapulpal injection
precipitation
push out bond strength
sealer
url https://jcdr.net/articles/PDF/13835/43932_F(KM)_CE[Ra1]_(SHU)_PF1(AKA_KM)_GC(Suk_SL)_PN(SL).pdf
work_keys_str_mv AT urmilabanshbahadurchauhan aninvitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT tipturmanjunathmangala aninvitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT rushikeshmahaparale aninvitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT adishsaraf aninvitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT snehamali aninvitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT sagarpawar aninvitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT vinciadsouza aninvitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT shubhammandhane aninvitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT urmilabanshbahadurchauhan invitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT tipturmanjunathmangala invitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT rushikeshmahaparale invitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT adishsaraf invitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT snehamali invitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT sagarpawar invitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT vinciadsouza invitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation
AT shubhammandhane invitroassessmentofeffectofinteractionbetweenlocalanaestheticsolutionwithsodiumhypochloriteonthesealingabilityofrootcanalobturation