Heterologous Expression of Arabidopsis AtARA6 in Soybean Enhances Salt Tolerance
Salt damage is an important abiotic stress affecting the agronomic traits of soybean. Soybeans rapidly sense and transmit adverse signals when salt-damaged, inducing a set of response mechanisms to resist salt stress. AtARA6 encodes a small GTPase, which plays an important role in Arabidopsis vesicl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.849357/full |
| _version_ | 1828108869420711936 |
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| author | Zhipeng Hong Yang Li Yang Zhao Mingyu Yang Xiaoming Zhang Yuhan Teng Linjie Jing Danxun Kong Tongxin Liu Shuanglin Li Fanli Meng Qi Wang Ling Zhang |
| author_facet | Zhipeng Hong Yang Li Yang Zhao Mingyu Yang Xiaoming Zhang Yuhan Teng Linjie Jing Danxun Kong Tongxin Liu Shuanglin Li Fanli Meng Qi Wang Ling Zhang |
| author_sort | Zhipeng Hong |
| collection | DOAJ |
| description | Salt damage is an important abiotic stress affecting the agronomic traits of soybean. Soybeans rapidly sense and transmit adverse signals when salt-damaged, inducing a set of response mechanisms to resist salt stress. AtARA6 encodes a small GTPase, which plays an important role in Arabidopsis vesicle transport and salt tolerance. In this study, we transformed the Arabidopsis gene AtARA6 into the cultivated soybean Shen Nong 9 (SN9). To investigate the salt tolerance pathways affected by AtARA6 in soybean, we performed transcriptome sequencing using transgenic soybean and wild-type (SN9) under salt treatment and water treatment. Our results suggest that AtARA6 is involved in the regulation of soybean SNARE complexes in the vesicle transport pathway, which may directly strengthen salt tolerance. In addition, we comprehensively analyzed the RNA-seq data of transgenic soybean and SN9 under different treatments and obtained 935 DEGs. GO analysis showed that these DEGs were significantly enriched in transcription factor activity, sequence-specific DNA binding, and the inositol catabolic process. Three salt-responsive negative regulator transcription factors, namely MYC2, WRKY6, and WRKY86, were found to be significantly downregulated after salt treatment in transgenic soybeans. Moreover, four genes encoding inositol oxygenase were significantly enriched in the inositol catabolic process pathway, which could improve the salt tolerance of transgenic soybeans by reducing their reactive oxygen species content. These are unique salt tolerance effects produced by transgenic soybeans. Our results provide basic insights into the function of AtARA6 in soybeans and its role in abiotic stress processes in plants. |
| first_indexed | 2024-04-11T10:52:44Z |
| format | Article |
| id | doaj.art-07b947e6aee544c3afd84490e45636d2 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T10:52:44Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-07b947e6aee544c3afd84490e45636d22022-12-22T04:28:51ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-05-011310.3389/fgene.2022.849357849357Heterologous Expression of Arabidopsis AtARA6 in Soybean Enhances Salt ToleranceZhipeng Hong0Yang Li1Yang Zhao2Mingyu Yang3Xiaoming Zhang4Yuhan Teng5Linjie Jing6Danxun Kong7Tongxin Liu8Shuanglin Li9Fanli Meng10Qi Wang11Ling Zhang12Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, ChinaInstitute of Crop Cultivation and Tillage, Heilongjiang Academy of Agricultural Sciences, Harbin, ChinaAgro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, ChinaSalt damage is an important abiotic stress affecting the agronomic traits of soybean. Soybeans rapidly sense and transmit adverse signals when salt-damaged, inducing a set of response mechanisms to resist salt stress. AtARA6 encodes a small GTPase, which plays an important role in Arabidopsis vesicle transport and salt tolerance. In this study, we transformed the Arabidopsis gene AtARA6 into the cultivated soybean Shen Nong 9 (SN9). To investigate the salt tolerance pathways affected by AtARA6 in soybean, we performed transcriptome sequencing using transgenic soybean and wild-type (SN9) under salt treatment and water treatment. Our results suggest that AtARA6 is involved in the regulation of soybean SNARE complexes in the vesicle transport pathway, which may directly strengthen salt tolerance. In addition, we comprehensively analyzed the RNA-seq data of transgenic soybean and SN9 under different treatments and obtained 935 DEGs. GO analysis showed that these DEGs were significantly enriched in transcription factor activity, sequence-specific DNA binding, and the inositol catabolic process. Three salt-responsive negative regulator transcription factors, namely MYC2, WRKY6, and WRKY86, were found to be significantly downregulated after salt treatment in transgenic soybeans. Moreover, four genes encoding inositol oxygenase were significantly enriched in the inositol catabolic process pathway, which could improve the salt tolerance of transgenic soybeans by reducing their reactive oxygen species content. These are unique salt tolerance effects produced by transgenic soybeans. Our results provide basic insights into the function of AtARA6 in soybeans and its role in abiotic stress processes in plants.https://www.frontiersin.org/articles/10.3389/fgene.2022.849357/fullsoybeanAtARA6salt toleranceRAB GTPaseRAB5SNARE pathway |
| spellingShingle | Zhipeng Hong Yang Li Yang Zhao Mingyu Yang Xiaoming Zhang Yuhan Teng Linjie Jing Danxun Kong Tongxin Liu Shuanglin Li Fanli Meng Qi Wang Ling Zhang Heterologous Expression of Arabidopsis AtARA6 in Soybean Enhances Salt Tolerance Frontiers in Genetics soybean AtARA6 salt tolerance RAB GTPase RAB5 SNARE pathway |
| title | Heterologous Expression of Arabidopsis AtARA6 in Soybean Enhances Salt Tolerance |
| title_full | Heterologous Expression of Arabidopsis AtARA6 in Soybean Enhances Salt Tolerance |
| title_fullStr | Heterologous Expression of Arabidopsis AtARA6 in Soybean Enhances Salt Tolerance |
| title_full_unstemmed | Heterologous Expression of Arabidopsis AtARA6 in Soybean Enhances Salt Tolerance |
| title_short | Heterologous Expression of Arabidopsis AtARA6 in Soybean Enhances Salt Tolerance |
| title_sort | heterologous expression of arabidopsis atara6 in soybean enhances salt tolerance |
| topic | soybean AtARA6 salt tolerance RAB GTPase RAB5 SNARE pathway |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.849357/full |
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