The protective effect of crocin on cisplatin-induced testicular impairment in rats
Abstract Background Side effects of cisplatin (CIS) such as testicular toxicity restrict its clinical use. Instead, evidence indicates that crocin (CR) has synergistic anti-cancer potential with CIS and exhibited beneficial effects on CIS-induced hepatorenal damage. The aim of this study was to inve...
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | BMC Urology |
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| Online Access: | https://doi.org/10.1186/s12894-021-00889-2 |
| _version_ | 1818578646920593408 |
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| author | Behzad Mesbahzadeh Mohammadmehdi Hassanzadeh-Taheri Mohadese-sadat Aliparast Pardis Baniasadi Mehran Hosseini |
| author_facet | Behzad Mesbahzadeh Mohammadmehdi Hassanzadeh-Taheri Mohadese-sadat Aliparast Pardis Baniasadi Mehran Hosseini |
| author_sort | Behzad Mesbahzadeh |
| collection | DOAJ |
| description | Abstract Background Side effects of cisplatin (CIS) such as testicular toxicity restrict its clinical use. Instead, evidence indicates that crocin (CR) has synergistic anti-cancer potential with CIS and exhibited beneficial effects on CIS-induced hepatorenal damage. The aim of this study was to investigate the protective potential of CR against CIS-induced testicular toxicity in rats. Methods Fifty adult male Wistar rats randomly assigned to five equal groups including control, CIS, and CIS plus CR at doses of 6.25 mg/kg (CIS + CR6.25), 25 mg/kg (CIS + CR25), and 100 mg/kg (CIS + CR100). CIS and CIS + CR groups received a single intraperitoneally (i.p.) injection of CIS (7 mg/kg). CR (6.25–100 mg/kg i.p.) injections were started three days before the CIS injection and continued once a day for up to 13 days. On the 14th day, all animals were sacrificed and their blood samples and testes were removed for biochemical and histological analyses. Results Compared to the control group, CIS significantly decreased relative testis weight (0.28 vs. 0.39, p < 0.001), testosterone level (0.3 vs. 2.31 ng/mL, p < 0.001), germinal layer area (25,886 vs. 35,320 µm2, p < 0.001), superoxide dismutase (SOD) (0.9 vs.1.73 U/mg, p < 0.001) and increased testicular lipid peroxidation (3.05 vs. 15.35 nmol/mg, p < 0.001). CR at 25 mg/kg ameliorated testicular lipid peroxidation and enhanced SOD activity compared to CIS group (p < 0.05). Besides, CR treatment at the maximum dose (100 mg/kg) resulted in reversing CIS effects on testis weight, testosterone level, SOD, lipid peroxidation, and germinal layer area. Conclusions These findings demonstrated that CR co-treatment could prevent CIS-induced testicular toxicity in rats. |
| first_indexed | 2024-12-16T06:49:07Z |
| format | Article |
| id | doaj.art-07ba4dd2b0f845e6bd01002d0e7abc27 |
| institution | Directory Open Access Journal |
| issn | 1471-2490 |
| language | English |
| last_indexed | 2024-12-16T06:49:07Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Urology |
| spelling | doaj.art-07ba4dd2b0f845e6bd01002d0e7abc272022-12-21T22:40:26ZengBMCBMC Urology1471-24902021-09-012111910.1186/s12894-021-00889-2The protective effect of crocin on cisplatin-induced testicular impairment in ratsBehzad Mesbahzadeh0Mohammadmehdi Hassanzadeh-Taheri1Mohadese-sadat Aliparast2Pardis Baniasadi3Mehran Hosseini4Department of Physiology, School of Allied Medical Sciences, Birjand University of Medical SciencesCellular and Molecular Research Center, Birjand University of Medical SciencesStudent Research Committee, Birjand University of Medical SciencesStudent Research Committee, Birjand University of Medical SciencesCellular and Molecular Research Center, Birjand University of Medical SciencesAbstract Background Side effects of cisplatin (CIS) such as testicular toxicity restrict its clinical use. Instead, evidence indicates that crocin (CR) has synergistic anti-cancer potential with CIS and exhibited beneficial effects on CIS-induced hepatorenal damage. The aim of this study was to investigate the protective potential of CR against CIS-induced testicular toxicity in rats. Methods Fifty adult male Wistar rats randomly assigned to five equal groups including control, CIS, and CIS plus CR at doses of 6.25 mg/kg (CIS + CR6.25), 25 mg/kg (CIS + CR25), and 100 mg/kg (CIS + CR100). CIS and CIS + CR groups received a single intraperitoneally (i.p.) injection of CIS (7 mg/kg). CR (6.25–100 mg/kg i.p.) injections were started three days before the CIS injection and continued once a day for up to 13 days. On the 14th day, all animals were sacrificed and their blood samples and testes were removed for biochemical and histological analyses. Results Compared to the control group, CIS significantly decreased relative testis weight (0.28 vs. 0.39, p < 0.001), testosterone level (0.3 vs. 2.31 ng/mL, p < 0.001), germinal layer area (25,886 vs. 35,320 µm2, p < 0.001), superoxide dismutase (SOD) (0.9 vs.1.73 U/mg, p < 0.001) and increased testicular lipid peroxidation (3.05 vs. 15.35 nmol/mg, p < 0.001). CR at 25 mg/kg ameliorated testicular lipid peroxidation and enhanced SOD activity compared to CIS group (p < 0.05). Besides, CR treatment at the maximum dose (100 mg/kg) resulted in reversing CIS effects on testis weight, testosterone level, SOD, lipid peroxidation, and germinal layer area. Conclusions These findings demonstrated that CR co-treatment could prevent CIS-induced testicular toxicity in rats.https://doi.org/10.1186/s12894-021-00889-2CisplatinCrocinGerminal epitheliumLipid peroxidationTestis |
| spellingShingle | Behzad Mesbahzadeh Mohammadmehdi Hassanzadeh-Taheri Mohadese-sadat Aliparast Pardis Baniasadi Mehran Hosseini The protective effect of crocin on cisplatin-induced testicular impairment in rats BMC Urology Cisplatin Crocin Germinal epithelium Lipid peroxidation Testis |
| title | The protective effect of crocin on cisplatin-induced testicular impairment in rats |
| title_full | The protective effect of crocin on cisplatin-induced testicular impairment in rats |
| title_fullStr | The protective effect of crocin on cisplatin-induced testicular impairment in rats |
| title_full_unstemmed | The protective effect of crocin on cisplatin-induced testicular impairment in rats |
| title_short | The protective effect of crocin on cisplatin-induced testicular impairment in rats |
| title_sort | protective effect of crocin on cisplatin induced testicular impairment in rats |
| topic | Cisplatin Crocin Germinal epithelium Lipid peroxidation Testis |
| url | https://doi.org/10.1186/s12894-021-00889-2 |
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