TraVis Pies: A Guide for Stable Isotope Metabolomics Interpretation Using an Intuitive Visualization
Tracer metabolomics is a powerful technology for the biomedical community to study and understand disease-inflicted metabolic mechanisms. However, the interpretation of tracer metabolomics results is highly technical, as the metabolites’ abundances, tracer incorporation and positions on the metaboli...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/12/7/593 |
| _version_ | 1797433296077979648 |
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| author | Sam De Craemer Karen Driesen Bart Ghesquière |
| author_facet | Sam De Craemer Karen Driesen Bart Ghesquière |
| author_sort | Sam De Craemer |
| collection | DOAJ |
| description | Tracer metabolomics is a powerful technology for the biomedical community to study and understand disease-inflicted metabolic mechanisms. However, the interpretation of tracer metabolomics results is highly technical, as the metabolites’ abundances, tracer incorporation and positions on the metabolic map all must be jointly interpreted. The field is currently lacking a structured approach to help less experienced researchers start the interpretation of tracer metabolomics datasets. We propose an approach using an intuitive visualization concept aided by a novel open-source tool, and provide guidelines on how researchers can apply the approach and the visualization tool to their own datasets. Using a showcase experiment, we demonstrate that the visualization approach leads to an intuitive interpretation that can ease researchers into understanding their tracer metabolomics data. |
| first_indexed | 2024-03-09T10:15:01Z |
| format | Article |
| id | doaj.art-07ba795ec5f34e1094ff17848214bd25 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T10:15:01Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-07ba795ec5f34e1094ff17848214bd252023-12-01T22:26:39ZengMDPI AGMetabolites2218-19892022-06-0112759310.3390/metabo12070593TraVis Pies: A Guide for Stable Isotope Metabolomics Interpretation Using an Intuitive VisualizationSam De Craemer0Karen Driesen1Bart Ghesquière2Metabolomics Expertise Center, VIB Center for Cancer Biology, 3000 Leuven, BelgiumMetabolomics Expertise Center, VIB Center for Cancer Biology, 3000 Leuven, BelgiumMetabolomics Expertise Center, VIB Center for Cancer Biology, 3000 Leuven, BelgiumTracer metabolomics is a powerful technology for the biomedical community to study and understand disease-inflicted metabolic mechanisms. However, the interpretation of tracer metabolomics results is highly technical, as the metabolites’ abundances, tracer incorporation and positions on the metabolic map all must be jointly interpreted. The field is currently lacking a structured approach to help less experienced researchers start the interpretation of tracer metabolomics datasets. We propose an approach using an intuitive visualization concept aided by a novel open-source tool, and provide guidelines on how researchers can apply the approach and the visualization tool to their own datasets. Using a showcase experiment, we demonstrate that the visualization approach leads to an intuitive interpretation that can ease researchers into understanding their tracer metabolomics data.https://www.mdpi.com/2218-1989/12/7/593tracer metabolomicsdata visualizationbiochemical pathways |
| spellingShingle | Sam De Craemer Karen Driesen Bart Ghesquière TraVis Pies: A Guide for Stable Isotope Metabolomics Interpretation Using an Intuitive Visualization Metabolites tracer metabolomics data visualization biochemical pathways |
| title | TraVis Pies: A Guide for Stable Isotope Metabolomics Interpretation Using an Intuitive Visualization |
| title_full | TraVis Pies: A Guide for Stable Isotope Metabolomics Interpretation Using an Intuitive Visualization |
| title_fullStr | TraVis Pies: A Guide for Stable Isotope Metabolomics Interpretation Using an Intuitive Visualization |
| title_full_unstemmed | TraVis Pies: A Guide for Stable Isotope Metabolomics Interpretation Using an Intuitive Visualization |
| title_short | TraVis Pies: A Guide for Stable Isotope Metabolomics Interpretation Using an Intuitive Visualization |
| title_sort | travis pies a guide for stable isotope metabolomics interpretation using an intuitive visualization |
| topic | tracer metabolomics data visualization biochemical pathways |
| url | https://www.mdpi.com/2218-1989/12/7/593 |
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