Naringenin inhibits the growth and stimulates the lignification of soybean root
The flavanone naringenin, an intermediate in flavonoid biosynthesis, was tested for its effect on root growth, phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities, as well as phenolic compounds and lignin contents in soybean (Glycine max L. Merrill) seedlings. Three-day-old seedlings w...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2010-06-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300005 |
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| author | Graciene de Souza Bido Maria de Lourdes Lucio Ferrarese Rogério Marchiosi Osvaldo Ferrarese-Filho |
| author_facet | Graciene de Souza Bido Maria de Lourdes Lucio Ferrarese Rogério Marchiosi Osvaldo Ferrarese-Filho |
| author_sort | Graciene de Souza Bido |
| collection | DOAJ |
| description | The flavanone naringenin, an intermediate in flavonoid biosynthesis, was tested for its effect on root growth, phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities, as well as phenolic compounds and lignin contents in soybean (Glycine max L. Merrill) seedlings. Three-day-old seedlings were cultivated in half-strength Hoagland nutrient solution (pH 6.0), with or without 0.1 to 0.4 mM naringenin in a growth chamber (25°C, 12-h photoperiod, irradiance of 280 µmol m-2 s-1) for 24 h. Inhibitory effects on root growth (length, weight, cell viability), PAL and soluble POD activities were detected after naringenin treatments. These effects were associated with stimulatory activity of the cell wall-bound POD followed by an increase in the lignin contents, suggesting that naringenin-induced inhibition in soybean roots could be due to the lignification process.<br>Os efeitos de naringenina, um intermediário da biossíntese de flavonóides, foram avaliados sobre o crescimento das raízes, as atividades da fenilalanina amônia liase (PAL) e peroxidases, bem como sobre os teores de compostos fenólicos e de lignina em plântulas de soja (Glycine max L. Merrill). Plântulas de três dias foram cultivadas em solução nutritiva de Hoagland, meia-força (pH 6,0), contendo ou não, naringenina 0,1 a 0,4 mM, em uma câmara de germinação (25°C, fotoperíodo de 12 h, 280 µmol m-2 s-1) durante 24 h. Efeitos inibitórios no crescimento das raízes (comprimento, massa e viabilidade celular) e nas atividades da PAL e POD solúvel foram constatados após os tratamentos com naringenina. Estes efeitos foram associados com atividade estimulatória da POD ligada à parede celular, seguido por aumento nos teores de lignina, sugerindo que a inibição do crescimento das raízes pode ser devido ao processo de lignificação. |
| first_indexed | 2024-12-11T06:48:36Z |
| format | Article |
| id | doaj.art-07c036192bae46d18e911103a9025cce |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-11T06:48:36Z |
| publishDate | 2010-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-07c036192bae46d18e911103a9025cce2022-12-22T01:16:58ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-06-0153353354210.1590/S1516-89132010000300005Naringenin inhibits the growth and stimulates the lignification of soybean rootGraciene de Souza BidoMaria de Lourdes Lucio FerrareseRogério MarchiosiOsvaldo Ferrarese-FilhoThe flavanone naringenin, an intermediate in flavonoid biosynthesis, was tested for its effect on root growth, phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities, as well as phenolic compounds and lignin contents in soybean (Glycine max L. Merrill) seedlings. Three-day-old seedlings were cultivated in half-strength Hoagland nutrient solution (pH 6.0), with or without 0.1 to 0.4 mM naringenin in a growth chamber (25°C, 12-h photoperiod, irradiance of 280 µmol m-2 s-1) for 24 h. Inhibitory effects on root growth (length, weight, cell viability), PAL and soluble POD activities were detected after naringenin treatments. These effects were associated with stimulatory activity of the cell wall-bound POD followed by an increase in the lignin contents, suggesting that naringenin-induced inhibition in soybean roots could be due to the lignification process.<br>Os efeitos de naringenina, um intermediário da biossíntese de flavonóides, foram avaliados sobre o crescimento das raízes, as atividades da fenilalanina amônia liase (PAL) e peroxidases, bem como sobre os teores de compostos fenólicos e de lignina em plântulas de soja (Glycine max L. Merrill). Plântulas de três dias foram cultivadas em solução nutritiva de Hoagland, meia-força (pH 6,0), contendo ou não, naringenina 0,1 a 0,4 mM, em uma câmara de germinação (25°C, fotoperíodo de 12 h, 280 µmol m-2 s-1) durante 24 h. Efeitos inibitórios no crescimento das raízes (comprimento, massa e viabilidade celular) e nas atividades da PAL e POD solúvel foram constatados após os tratamentos com naringenina. Estes efeitos foram associados com atividade estimulatória da POD ligada à parede celular, seguido por aumento nos teores de lignina, sugerindo que a inibição do crescimento das raízes pode ser devido ao processo de lignificação.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300005Allelopathyligninflavonoidperoxidasephenylalanine ammonia-lyaseroot growth |
| spellingShingle | Graciene de Souza Bido Maria de Lourdes Lucio Ferrarese Rogério Marchiosi Osvaldo Ferrarese-Filho Naringenin inhibits the growth and stimulates the lignification of soybean root Brazilian Archives of Biology and Technology Allelopathy lignin flavonoid peroxidase phenylalanine ammonia-lyase root growth |
| title | Naringenin inhibits the growth and stimulates the lignification of soybean root |
| title_full | Naringenin inhibits the growth and stimulates the lignification of soybean root |
| title_fullStr | Naringenin inhibits the growth and stimulates the lignification of soybean root |
| title_full_unstemmed | Naringenin inhibits the growth and stimulates the lignification of soybean root |
| title_short | Naringenin inhibits the growth and stimulates the lignification of soybean root |
| title_sort | naringenin inhibits the growth and stimulates the lignification of soybean root |
| topic | Allelopathy lignin flavonoid peroxidase phenylalanine ammonia-lyase root growth |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300005 |
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