Untargeted Metabolomics Reveal Defensome-Related Metabolic Reprogramming in Sorghum bicolor against Infection by Burkholderia andropogonis
Burkholderia andropogonis is the causal agent of bacterial leaf stripe, one of the three major bacterial diseases affecting Sorghum bicolor. However, the biochemical aspects of the pathophysiological host responses are not well understood. An untargeted metabolomics approach was designed to understa...
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| Format: | Article |
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MDPI AG
2019-01-01
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| Series: | Metabolites |
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| Online Access: | http://www.mdpi.com/2218-1989/9/1/8 |
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| author | Charity R. Mareya Fidele Tugizimana Lizelle A. Piater Ntakadzeni E. Madala Paul A. Steenkamp Ian A. Dubery |
| author_facet | Charity R. Mareya Fidele Tugizimana Lizelle A. Piater Ntakadzeni E. Madala Paul A. Steenkamp Ian A. Dubery |
| author_sort | Charity R. Mareya |
| collection | DOAJ |
| description | Burkholderia andropogonis is the causal agent of bacterial leaf stripe, one of the three major bacterial diseases affecting Sorghum bicolor. However, the biochemical aspects of the pathophysiological host responses are not well understood. An untargeted metabolomics approach was designed to understand molecular mechanisms underlying S. bicolor–B. andropogonis interactions. At the 4-leaf stage, two sorghum cultivars (NS 5511 and NS 5655) differing in disease tolerance, were infected with B. andropogonis and the metabolic changes monitored over time. The NS 5511 cultivar displayed delayed signs of wilting and lesion progression compared to the NS 5655 cultivar, indicative of enhanced resistance. The metabolomics results identified statistically significant metabolites as biomarkers associated with the sorghum defence. These include the phytohormones salicylic acid, jasmonic acid, and zeatin. Moreover, metabolic reprogramming in an array of chemically diverse metabolites that span a wide range of metabolic pathways was associated with the defence response. Signatory biomarkers included aromatic amino acids, shikimic acid, metabolites from the phenylpropanoid and flavonoid pathways, as well as fatty acids. Enhanced synthesis and accumulation of apigenin and derivatives thereof was a prominent feature of the altered metabolomes. The analyses revealed an intricate and dynamic network of the sorghum defence arsenal towards B. andropogonis in establishing an enhanced defensive capacity in support of resistance and disease suppression. The results pave the way for future analysis of the biosynthesis of signatory biomarkers and regulation of relevant metabolic pathways in sorghum. |
| first_indexed | 2024-04-13T01:22:49Z |
| format | Article |
| id | doaj.art-07c249fd55ba4be28ed6a181c92e6d17 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-04-13T01:22:49Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-07c249fd55ba4be28ed6a181c92e6d172022-12-22T03:08:42ZengMDPI AGMetabolites2218-19892019-01-0191810.3390/metabo9010008metabo9010008Untargeted Metabolomics Reveal Defensome-Related Metabolic Reprogramming in Sorghum bicolor against Infection by Burkholderia andropogonisCharity R. Mareya0Fidele Tugizimana1Lizelle A. Piater2Ntakadzeni E. Madala3Paul A. Steenkamp4Ian A. Dubery5Centre for Plant Metabolomics Research, Department of Biochemistry, University of Johannesburg, Auckland Park 2006, Johannesburg, South AfricaCentre for Plant Metabolomics Research, Department of Biochemistry, University of Johannesburg, Auckland Park 2006, Johannesburg, South AfricaCentre for Plant Metabolomics Research, Department of Biochemistry, University of Johannesburg, Auckland Park 2006, Johannesburg, South AfricaCentre for Plant Metabolomics Research, Department of Biochemistry, University of Johannesburg, Auckland Park 2006, Johannesburg, South AfricaCentre for Plant Metabolomics Research, Department of Biochemistry, University of Johannesburg, Auckland Park 2006, Johannesburg, South AfricaCentre for Plant Metabolomics Research, Department of Biochemistry, University of Johannesburg, Auckland Park 2006, Johannesburg, South AfricaBurkholderia andropogonis is the causal agent of bacterial leaf stripe, one of the three major bacterial diseases affecting Sorghum bicolor. However, the biochemical aspects of the pathophysiological host responses are not well understood. An untargeted metabolomics approach was designed to understand molecular mechanisms underlying S. bicolor–B. andropogonis interactions. At the 4-leaf stage, two sorghum cultivars (NS 5511 and NS 5655) differing in disease tolerance, were infected with B. andropogonis and the metabolic changes monitored over time. The NS 5511 cultivar displayed delayed signs of wilting and lesion progression compared to the NS 5655 cultivar, indicative of enhanced resistance. The metabolomics results identified statistically significant metabolites as biomarkers associated with the sorghum defence. These include the phytohormones salicylic acid, jasmonic acid, and zeatin. Moreover, metabolic reprogramming in an array of chemically diverse metabolites that span a wide range of metabolic pathways was associated with the defence response. Signatory biomarkers included aromatic amino acids, shikimic acid, metabolites from the phenylpropanoid and flavonoid pathways, as well as fatty acids. Enhanced synthesis and accumulation of apigenin and derivatives thereof was a prominent feature of the altered metabolomes. The analyses revealed an intricate and dynamic network of the sorghum defence arsenal towards B. andropogonis in establishing an enhanced defensive capacity in support of resistance and disease suppression. The results pave the way for future analysis of the biosynthesis of signatory biomarkers and regulation of relevant metabolic pathways in sorghum.http://www.mdpi.com/2218-1989/9/1/8Burkholderia andropogonisdefenceflavonoidsmetabolomicsphytochemicalsphytohormonesphytoalexinsSorghum bicolor |
| spellingShingle | Charity R. Mareya Fidele Tugizimana Lizelle A. Piater Ntakadzeni E. Madala Paul A. Steenkamp Ian A. Dubery Untargeted Metabolomics Reveal Defensome-Related Metabolic Reprogramming in Sorghum bicolor against Infection by Burkholderia andropogonis Metabolites Burkholderia andropogonis defence flavonoids metabolomics phytochemicals phytohormones phytoalexins Sorghum bicolor |
| title | Untargeted Metabolomics Reveal Defensome-Related Metabolic Reprogramming in Sorghum bicolor against Infection by Burkholderia andropogonis |
| title_full | Untargeted Metabolomics Reveal Defensome-Related Metabolic Reprogramming in Sorghum bicolor against Infection by Burkholderia andropogonis |
| title_fullStr | Untargeted Metabolomics Reveal Defensome-Related Metabolic Reprogramming in Sorghum bicolor against Infection by Burkholderia andropogonis |
| title_full_unstemmed | Untargeted Metabolomics Reveal Defensome-Related Metabolic Reprogramming in Sorghum bicolor against Infection by Burkholderia andropogonis |
| title_short | Untargeted Metabolomics Reveal Defensome-Related Metabolic Reprogramming in Sorghum bicolor against Infection by Burkholderia andropogonis |
| title_sort | untargeted metabolomics reveal defensome related metabolic reprogramming in sorghum bicolor against infection by burkholderia andropogonis |
| topic | Burkholderia andropogonis defence flavonoids metabolomics phytochemicals phytohormones phytoalexins Sorghum bicolor |
| url | http://www.mdpi.com/2218-1989/9/1/8 |
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