Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism.
Upon water loss, some organisms pause their life cycles and escape death. While widespread in microbes, this is less common in animals. Aedes mosquitoes are vectors for viral diseases. Aedes eggs can survive dry environments, but molecular and cellular principles enabling egg survival through desicc...
| Главные авторы: | , , , |
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| Формат: | Статья |
| Язык: | English |
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Public Library of Science (PLoS)
2023-10-01
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| Серии: | PLoS Biology |
| Online-ссылка: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3002342&type=printable |
| _version_ | 1827096502277767168 |
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| author | Anjana Prasad Sreesa Sreedharan Baskar Bakthavachalu Sunil Laxman |
| author_facet | Anjana Prasad Sreesa Sreedharan Baskar Bakthavachalu Sunil Laxman |
| author_sort | Anjana Prasad |
| collection | DOAJ |
| description | Upon water loss, some organisms pause their life cycles and escape death. While widespread in microbes, this is less common in animals. Aedes mosquitoes are vectors for viral diseases. Aedes eggs can survive dry environments, but molecular and cellular principles enabling egg survival through desiccation remain unknown. In this report, we find that Aedes aegypti eggs, in contrast to Anopheles stephensi, survive desiccation by acquiring desiccation tolerance at a late developmental stage. We uncover unique proteome and metabolic state changes in Aedes embryos during desiccation that reflect reduced central carbon metabolism, rewiring towards polyamine production, and enhanced lipid utilisation for energy and polyamine synthesis. Using inhibitors targeting these processes in blood-fed mosquitoes that lay eggs, we infer a two-step process of desiccation tolerance in Aedes eggs. The metabolic rewiring towards lipid breakdown and dependent polyamine accumulation confers resistance to desiccation. Furthermore, rapid lipid breakdown is required to fuel energetic requirements upon water reentry to enable larval hatching and survival upon rehydration. This study is fundamental to understanding Aedes embryo survival and in controlling the spread of these mosquitoes. |
| first_indexed | 2024-03-08T03:10:26Z |
| format | Article |
| id | doaj.art-07c31e3d908e40a4b7c32ea30e40b51a |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2025-03-20T07:16:47Z |
| publishDate | 2023-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-07c31e3d908e40a4b7c32ea30e40b51a2024-10-01T05:31:04ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852023-10-012110e300234210.1371/journal.pbio.3002342Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism.Anjana PrasadSreesa SreedharanBaskar BakthavachaluSunil LaxmanUpon water loss, some organisms pause their life cycles and escape death. While widespread in microbes, this is less common in animals. Aedes mosquitoes are vectors for viral diseases. Aedes eggs can survive dry environments, but molecular and cellular principles enabling egg survival through desiccation remain unknown. In this report, we find that Aedes aegypti eggs, in contrast to Anopheles stephensi, survive desiccation by acquiring desiccation tolerance at a late developmental stage. We uncover unique proteome and metabolic state changes in Aedes embryos during desiccation that reflect reduced central carbon metabolism, rewiring towards polyamine production, and enhanced lipid utilisation for energy and polyamine synthesis. Using inhibitors targeting these processes in blood-fed mosquitoes that lay eggs, we infer a two-step process of desiccation tolerance in Aedes eggs. The metabolic rewiring towards lipid breakdown and dependent polyamine accumulation confers resistance to desiccation. Furthermore, rapid lipid breakdown is required to fuel energetic requirements upon water reentry to enable larval hatching and survival upon rehydration. This study is fundamental to understanding Aedes embryo survival and in controlling the spread of these mosquitoes.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3002342&type=printable |
| spellingShingle | Anjana Prasad Sreesa Sreedharan Baskar Bakthavachalu Sunil Laxman Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism. PLoS Biology |
| title | Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism. |
| title_full | Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism. |
| title_fullStr | Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism. |
| title_full_unstemmed | Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism. |
| title_short | Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism. |
| title_sort | eggs of the mosquito aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3002342&type=printable |
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