In vitro model of the blood-brain barrier established by co-culture of primary sheep cerebral microvascular endothelial and astrocyte cells
Blood-brain barrier (BBB) characteristics are induced and maintained by cross-talk between brain microvessel endothelial cells and neighbouring elements of the neurovascular unit. It plays a primary role in a selective diffusion barrier at the level of the cerebral microvascular endothelium. Here, w...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2018-03-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2350 |
| _version_ | 1797800612953325568 |
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| author | Jingjing REN Mingwei YANG Genqiang YAN Jianjun JIANG Pengyan WANG |
| author_facet | Jingjing REN Mingwei YANG Genqiang YAN Jianjun JIANG Pengyan WANG |
| author_sort | Jingjing REN |
| collection | DOAJ |
| description | Blood-brain barrier (BBB) characteristics are induced and maintained by cross-talk between brain microvessel endothelial cells and
neighbouring elements of the neurovascular unit. It plays a primary role in a selective diffusion barrier at the level of the cerebral
microvascular endothelium. Here, we have developed and characterized an in vitro model of the blood-brain barrier by seeding primary
brain microvascular endothelial cells (BMECs) and astrocyte cells (ACs) from sheep on a polyester Transwell cell culture membrane with
0.4-μm pores, and conducted transepithelial electrical resistance measurements (TEER), leakage tests and assays for the permeability of
horseradish peroxidase (HRP). The results of TEER and 4-h leakage assay showed that the new syngeneic BBB model had a characteristic
of BBB in vivo. The HRP permeability test showed that the BBB model had good barrier function. Results showed that our model may be a
valuable tool in the study of the mechanisms of brain invasion mediated by internalins of Listeria monocytogenes. |
| first_indexed | 2024-03-13T04:36:55Z |
| format | Article |
| id | doaj.art-07c830aebca042bab13dce9b8e25cd79 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:36:55Z |
| publishDate | 2018-03-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-07c830aebca042bab13dce9b8e25cd792023-06-19T06:43:42ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512018-03-0124337938610.9775/kvfd.2017.190032350In vitro model of the blood-brain barrier established by co-culture of primary sheep cerebral microvascular endothelial and astrocyte cellsJingjing REN0Mingwei YANG1Genqiang YAN2Jianjun JIANG3Pengyan WANG4College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, P. R. CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, P. R. CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, P. R. CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, P. R. CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, P. R. CHINABlood-brain barrier (BBB) characteristics are induced and maintained by cross-talk between brain microvessel endothelial cells and neighbouring elements of the neurovascular unit. It plays a primary role in a selective diffusion barrier at the level of the cerebral microvascular endothelium. Here, we have developed and characterized an in vitro model of the blood-brain barrier by seeding primary brain microvascular endothelial cells (BMECs) and astrocyte cells (ACs) from sheep on a polyester Transwell cell culture membrane with 0.4-μm pores, and conducted transepithelial electrical resistance measurements (TEER), leakage tests and assays for the permeability of horseradish peroxidase (HRP). The results of TEER and 4-h leakage assay showed that the new syngeneic BBB model had a characteristic of BBB in vivo. The HRP permeability test showed that the BBB model had good barrier function. Results showed that our model may be a valuable tool in the study of the mechanisms of brain invasion mediated by internalins of Listeria monocytogenes.https://vetdergikafkas.org/pdf.php?id=2350blood-brain barrierastrocytesbrain microvascular endothelial cellsco-culturein vitro bbb modelsheep |
| spellingShingle | Jingjing REN Mingwei YANG Genqiang YAN Jianjun JIANG Pengyan WANG In vitro model of the blood-brain barrier established by co-culture of primary sheep cerebral microvascular endothelial and astrocyte cells Kafkas Universitesi Veteriner Fakültesi Dergisi blood-brain barrier astrocytes brain microvascular endothelial cells co-culture in vitro bbb model sheep |
| title | In vitro model of the blood-brain barrier established by co-culture of primary sheep cerebral microvascular endothelial and astrocyte cells |
| title_full | In vitro model of the blood-brain barrier established by co-culture of primary sheep cerebral microvascular endothelial and astrocyte cells |
| title_fullStr | In vitro model of the blood-brain barrier established by co-culture of primary sheep cerebral microvascular endothelial and astrocyte cells |
| title_full_unstemmed | In vitro model of the blood-brain barrier established by co-culture of primary sheep cerebral microvascular endothelial and astrocyte cells |
| title_short | In vitro model of the blood-brain barrier established by co-culture of primary sheep cerebral microvascular endothelial and astrocyte cells |
| title_sort | in vitro model of the blood brain barrier established by co culture of primary sheep cerebral microvascular endothelial and astrocyte cells |
| topic | blood-brain barrier astrocytes brain microvascular endothelial cells co-culture in vitro bbb model sheep |
| url | https://vetdergikafkas.org/pdf.php?id=2350 |
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