Isolation and characterization of Streptococcus spp. group B in Nile tilapias (Oreochromis niloticus) reared in hapas nets and earth nurseries in the northern region of Parana State, Brazil
The objective of this study was to isolate and characterize Streptococcus spp. in Nile tilapias (Oreochromis niloticus) reared in net-pens and earth nurseries. Eight intensive tilapia-rearing farms were investigated in north Paraná, Brazil from April 1st 2001 to April 30th 2002. The fish were reared...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2005-01-01
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000600023 |
| _version_ | 1811307150156234752 |
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| author | Salvador Rogério Muller Ernst Eckehardt Freitas Julio César de Leonhadt Julio Hermann Pretto-Giordano Lucienne Garcia Dias Juliana Alves |
| author_facet | Salvador Rogério Muller Ernst Eckehardt Freitas Julio César de Leonhadt Julio Hermann Pretto-Giordano Lucienne Garcia Dias Juliana Alves |
| author_sort | Salvador Rogério |
| collection | DOAJ |
| description | The objective of this study was to isolate and characterize Streptococcus spp. in Nile tilapias (Oreochromis niloticus) reared in net-pens and earth nurseries. Eight intensive tilapia-rearing farms were investigated in north Paraná, Brazil from April 1st 2001 to April 30th 2002. The fish were reared in a system of hapas nets on four farms and in earth nurseries on other four farms. A total of 370 samples were analyzed of material collected from 120 fish (brain, liver, kidney, skin scrapes, ascites liquid and eye) that were sown on BHI agar (Brain Heart Infusion) supplemented with 1% yeast extract and sheep blood. Streptococcus spp. was isolated in 36 of the samples (18 brain, eight liver, eight kidney and two ascites liquid) from 25 fish. Streptococci were isolated in both systems, almost in the same proportion. First the streptococci were characterized by the catalase and esculin test, growth in methylene blue and sodium chloride at 6.5%. They were classified in groups by the Slidex Strepto-Kit (BioMerieux, France). The phenotypic characteristics were determined by the Api 20 Strep microtest system (BioMerieux, France). The 36 Streptococcus spp. samples did not present hemolysis and were classified as Lancefield group B. Further 16 samples were identified as Streptococcus agalactiae and 20 were not identified by the Api 20 Strep, but presented the same biochemical profile described for the reference strain of Streptococcus difficile (ND-2-22). |
| first_indexed | 2024-04-13T08:59:30Z |
| format | Article |
| id | doaj.art-07cb73086add424fa5fe3760000ec57a |
| institution | Directory Open Access Journal |
| issn | 0103-8478 1678-4596 |
| language | English |
| last_indexed | 2024-04-13T08:59:30Z |
| publishDate | 2005-01-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-07cb73086add424fa5fe3760000ec57a2022-12-22T02:53:10ZengUniversidade Federal de Santa MariaCiência Rural0103-84781678-45962005-01-0135613741378Isolation and characterization of Streptococcus spp. group B in Nile tilapias (Oreochromis niloticus) reared in hapas nets and earth nurseries in the northern region of Parana State, BrazilSalvador RogérioMuller Ernst EckehardtFreitas Julio César deLeonhadt Julio HermannPretto-Giordano Lucienne GarciaDias Juliana AlvesThe objective of this study was to isolate and characterize Streptococcus spp. in Nile tilapias (Oreochromis niloticus) reared in net-pens and earth nurseries. Eight intensive tilapia-rearing farms were investigated in north Paraná, Brazil from April 1st 2001 to April 30th 2002. The fish were reared in a system of hapas nets on four farms and in earth nurseries on other four farms. A total of 370 samples were analyzed of material collected from 120 fish (brain, liver, kidney, skin scrapes, ascites liquid and eye) that were sown on BHI agar (Brain Heart Infusion) supplemented with 1% yeast extract and sheep blood. Streptococcus spp. was isolated in 36 of the samples (18 brain, eight liver, eight kidney and two ascites liquid) from 25 fish. Streptococci were isolated in both systems, almost in the same proportion. First the streptococci were characterized by the catalase and esculin test, growth in methylene blue and sodium chloride at 6.5%. They were classified in groups by the Slidex Strepto-Kit (BioMerieux, France). The phenotypic characteristics were determined by the Api 20 Strep microtest system (BioMerieux, France). The 36 Streptococcus spp. samples did not present hemolysis and were classified as Lancefield group B. Further 16 samples were identified as Streptococcus agalactiae and 20 were not identified by the Api 20 Strep, but presented the same biochemical profile described for the reference strain of Streptococcus difficile (ND-2-22).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000600023pisciculturestreptococciinfectionbacteriology |
| spellingShingle | Salvador Rogério Muller Ernst Eckehardt Freitas Julio César de Leonhadt Julio Hermann Pretto-Giordano Lucienne Garcia Dias Juliana Alves Isolation and characterization of Streptococcus spp. group B in Nile tilapias (Oreochromis niloticus) reared in hapas nets and earth nurseries in the northern region of Parana State, Brazil Ciência Rural pisciculture streptococci infection bacteriology |
| title | Isolation and characterization of Streptococcus spp. group B in Nile tilapias (Oreochromis niloticus) reared in hapas nets and earth nurseries in the northern region of Parana State, Brazil |
| title_full | Isolation and characterization of Streptococcus spp. group B in Nile tilapias (Oreochromis niloticus) reared in hapas nets and earth nurseries in the northern region of Parana State, Brazil |
| title_fullStr | Isolation and characterization of Streptococcus spp. group B in Nile tilapias (Oreochromis niloticus) reared in hapas nets and earth nurseries in the northern region of Parana State, Brazil |
| title_full_unstemmed | Isolation and characterization of Streptococcus spp. group B in Nile tilapias (Oreochromis niloticus) reared in hapas nets and earth nurseries in the northern region of Parana State, Brazil |
| title_short | Isolation and characterization of Streptococcus spp. group B in Nile tilapias (Oreochromis niloticus) reared in hapas nets and earth nurseries in the northern region of Parana State, Brazil |
| title_sort | isolation and characterization of streptococcus spp group b in nile tilapias oreochromis niloticus reared in hapas nets and earth nurseries in the northern region of parana state brazil |
| topic | pisciculture streptococci infection bacteriology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000600023 |
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