Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut
Tissue homeostasis is maintained by differentiated progeny of residential stem cells. Both extrinsic signals and intrinsic factors play critical roles in the proliferation and differentiation of adult intestinal stem cells (ISCs). However, how extrinsic signals are transduced into ISCs still remains...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2019-10-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/8/10/bio047126 |
| _version_ | 1819075599947268096 |
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| author | Hubing Ma Huiqing Zhao Fuli Liu Hang Zhao Ruiyan Kong Lin Shi Min Wei Zhouhua Li |
| author_facet | Hubing Ma Huiqing Zhao Fuli Liu Hang Zhao Ruiyan Kong Lin Shi Min Wei Zhouhua Li |
| author_sort | Hubing Ma |
| collection | DOAJ |
| description | Tissue homeostasis is maintained by differentiated progeny of residential stem cells. Both extrinsic signals and intrinsic factors play critical roles in the proliferation and differentiation of adult intestinal stem cells (ISCs). However, how extrinsic signals are transduced into ISCs still remains unclear. Here, we find that heparan sulfate (HS), a class of glycosaminoglycan (GAG) chains, negatively regulates progenitor proliferation and differentiation to maintain midgut homeostasis under physiological conditions. Interestingly, HS depletion in progenitors results in inactivation of Decapentaplegic (Dpp) signaling. Dpp signal inactivation in progenitors resembles HS-deficient intestines. Ectopic Dpp signaling completely rescued the defects caused by HS depletion. Taken together, these data demonstrate that HS is required for Dpp signaling to maintain midgut homeostasis. Our results provide insight into the regulatory mechanisms of how extrinsic signals are transduced into stem cells to regulate their proliferation and differentiation. |
| first_indexed | 2024-12-21T18:27:59Z |
| format | Article |
| id | doaj.art-07cc4e003a0c4b788067428778b89430 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-21T18:27:59Z |
| publishDate | 2019-10-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-07cc4e003a0c4b788067428778b894302022-12-21T18:54:22ZengThe Company of BiologistsBiology Open2046-63902019-10-0181010.1242/bio.047126047126Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgutHubing Ma0Huiqing Zhao1Fuli Liu2Hang Zhao3Ruiyan Kong4Lin Shi5Min Wei6Zhouhua Li7 College of Life Sciences, Capital Normal University, Beijing 100048, China College of Life Sciences, Capital Normal University, Beijing 100048, China College of Life Sciences, Capital Normal University, Beijing 100048, China College of Life Sciences, Capital Normal University, Beijing 100048, China College of Life Sciences, Capital Normal University, Beijing 100048, China College of Life Sciences, Capital Normal University, Beijing 100048, China College of Life Sciences, Capital Normal University, Beijing 100048, China College of Life Sciences, Capital Normal University, Beijing 100048, China Tissue homeostasis is maintained by differentiated progeny of residential stem cells. Both extrinsic signals and intrinsic factors play critical roles in the proliferation and differentiation of adult intestinal stem cells (ISCs). However, how extrinsic signals are transduced into ISCs still remains unclear. Here, we find that heparan sulfate (HS), a class of glycosaminoglycan (GAG) chains, negatively regulates progenitor proliferation and differentiation to maintain midgut homeostasis under physiological conditions. Interestingly, HS depletion in progenitors results in inactivation of Decapentaplegic (Dpp) signaling. Dpp signal inactivation in progenitors resembles HS-deficient intestines. Ectopic Dpp signaling completely rescued the defects caused by HS depletion. Taken together, these data demonstrate that HS is required for Dpp signaling to maintain midgut homeostasis. Our results provide insight into the regulatory mechanisms of how extrinsic signals are transduced into stem cells to regulate their proliferation and differentiation.http://bio.biologists.org/content/8/10/bio047126intestinal stem cellheparan sulfatedrosophilatissue homeostasisdpp signaling |
| spellingShingle | Hubing Ma Huiqing Zhao Fuli Liu Hang Zhao Ruiyan Kong Lin Shi Min Wei Zhouhua Li Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut Biology Open intestinal stem cell heparan sulfate drosophila tissue homeostasis dpp signaling |
| title | Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut |
| title_full | Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut |
| title_fullStr | Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut |
| title_full_unstemmed | Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut |
| title_short | Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut |
| title_sort | heparan sulfate negatively regulates intestinal stem cell proliferation in drosophila adult midgut |
| topic | intestinal stem cell heparan sulfate drosophila tissue homeostasis dpp signaling |
| url | http://bio.biologists.org/content/8/10/bio047126 |
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