Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation
Decellularized tissue is generally considered immune privileged after transplantation and is an attractive scaffold type for tissue regeneration, including applications for infertility treatment. However, the immune response following transplantation of decellularized tissue is insufficiently studie...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2022-08-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314221118858 |
| _version_ | 1828149287004930048 |
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| author | Edina Sehic Emy Thorén Ingigerdur Gudmundsdottir Mihai Oltean Mats Brännström Mats Hellström |
| author_facet | Edina Sehic Emy Thorén Ingigerdur Gudmundsdottir Mihai Oltean Mats Brännström Mats Hellström |
| author_sort | Edina Sehic |
| collection | DOAJ |
| description | Decellularized tissue is generally considered immune privileged after transplantation and is an attractive scaffold type for tissue regeneration, including applications for infertility treatment. However, the immune response following transplantation of decellularized tissue is insufficiently studied, in particular after they have been recellularized with mesenchymal stem cells (MSCs). Therefore, we replaced a large uterus segment with a bioengineered graft developed from decellularized uterus tissue and analyzed the immune response during the first 4 months in acellular or MSCs-recellularized scaffolds in the rat. Immunohistochemistry-stained infiltrating immune cells and plasma levels for 16 cytokines and chemokines were quantified. Results revealed that MSCs created an advantageous microenvironment by increasing anti-inflammatory interleukin 10 levels, and increasing the population of FOXP3 + T Regs and CD163 + M2 macrophages, and by reducing the CD8 + cytotoxic T cell population. Hence, MSCs should be considered an immunotherapeutic cell source with the ability to dictate regeneration success after decellularized tissue transplantation. |
| first_indexed | 2024-04-11T21:27:18Z |
| format | Article |
| id | doaj.art-07cc953434684734acc23b574aaf3333 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-04-11T21:27:18Z |
| publishDate | 2022-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-07cc953434684734acc23b574aaf33332022-12-22T04:02:20ZengSAGE PublishingJournal of Tissue Engineering2041-73142022-08-011310.1177/20417314221118858Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantationEdina Sehic0Emy Thorén1Ingigerdur Gudmundsdottir2Mihai Oltean3Mats Brännström4Mats Hellström5Department of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDepartment of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDepartment of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDepartment of Surgery, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenStockholm IVF-EUGIN, Stockholm, SwedenDepartment of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDecellularized tissue is generally considered immune privileged after transplantation and is an attractive scaffold type for tissue regeneration, including applications for infertility treatment. However, the immune response following transplantation of decellularized tissue is insufficiently studied, in particular after they have been recellularized with mesenchymal stem cells (MSCs). Therefore, we replaced a large uterus segment with a bioengineered graft developed from decellularized uterus tissue and analyzed the immune response during the first 4 months in acellular or MSCs-recellularized scaffolds in the rat. Immunohistochemistry-stained infiltrating immune cells and plasma levels for 16 cytokines and chemokines were quantified. Results revealed that MSCs created an advantageous microenvironment by increasing anti-inflammatory interleukin 10 levels, and increasing the population of FOXP3 + T Regs and CD163 + M2 macrophages, and by reducing the CD8 + cytotoxic T cell population. Hence, MSCs should be considered an immunotherapeutic cell source with the ability to dictate regeneration success after decellularized tissue transplantation.https://doi.org/10.1177/20417314221118858 |
| spellingShingle | Edina Sehic Emy Thorén Ingigerdur Gudmundsdottir Mihai Oltean Mats Brännström Mats Hellström Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation Journal of Tissue Engineering |
| title | Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation |
| title_full | Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation |
| title_fullStr | Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation |
| title_full_unstemmed | Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation |
| title_short | Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation |
| title_sort | mesenchymal stem cells establish a pro regenerative immune milieu after decellularized rat uterus tissue transplantation |
| url | https://doi.org/10.1177/20417314221118858 |
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