Transcriptome Analysis Identifies Downstream Genes of <i>CLAVATA3</i> in Tomato
Fruit locule number is an important agronomic trait that affects fruit appearance, quality, and yield. <i>CLAVATA3</i> (<i>SlCLV3</i>) is a candidate gene of the <i>fasciated</i> (<i>fas</i>) locus that plays a role in controlling the number of flower...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/1/11 |
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| author | Yaofeng Zhang Huixian Sun Linlin Tian Yanxiu Miao Leiping Hou Meihua Sun Mingfang Qi Tianlai Li |
| author_facet | Yaofeng Zhang Huixian Sun Linlin Tian Yanxiu Miao Leiping Hou Meihua Sun Mingfang Qi Tianlai Li |
| author_sort | Yaofeng Zhang |
| collection | DOAJ |
| description | Fruit locule number is an important agronomic trait that affects fruit appearance, quality, and yield. <i>CLAVATA3</i> (<i>SlCLV3</i>) is a candidate gene of the <i>fasciated</i> (<i>fas</i>) locus that plays a role in controlling the number of flower organs and fruit locules in tomato. The <i>SlCLV3</i> encoding signal peptide mainly acts by inhibiting the expression of <i>WUSCHEL</i> (<i>WUS</i>) but there is little research about how the receptor transmits the CLV3 signal to <i>WUS</i> and inhibits its expression. The CRISPR/Cas9 method was employed to edit the first exon of tomato <i>SlCLV3</i> in this study, leading to the functional deletion of <i>SlCLV3</i>. As a result, flowers with a high number of organs and fruits with a high number of locules were produced. We screened six candidate genes using the transcriptome of <i>clv3</i> mutants, analyzed expression variations in these genes between the cultivated allele and wild-type allele of <i>fas</i>, and showed that only <i>SlLET6</i> and <i>SlGIF1</i> (<i>GRF1-interacting factor 1</i>) were influenced by the <i>fas</i> locus. <i>SlLET6</i> overexpression resulted in an increase in flower carpels and fruit locules. These results suggest that <i>SlLET6</i> may be the downstream gene of <i>SlCLV3</i> regulating the number of carpels and fruit locules in tomato. |
| first_indexed | 2024-03-08T10:48:54Z |
| format | Article |
| id | doaj.art-07d433fdfd644a24808b0fa298f09c2c |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-08T10:48:54Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-07d433fdfd644a24808b0fa298f09c2c2024-01-26T16:49:36ZengMDPI AGHorticulturae2311-75242023-12-011011110.3390/horticulturae10010011Transcriptome Analysis Identifies Downstream Genes of <i>CLAVATA3</i> in TomatoYaofeng Zhang0Huixian Sun1Linlin Tian2Yanxiu Miao3Leiping Hou4Meihua Sun5Mingfang Qi6Tianlai Li7College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaFruit locule number is an important agronomic trait that affects fruit appearance, quality, and yield. <i>CLAVATA3</i> (<i>SlCLV3</i>) is a candidate gene of the <i>fasciated</i> (<i>fas</i>) locus that plays a role in controlling the number of flower organs and fruit locules in tomato. The <i>SlCLV3</i> encoding signal peptide mainly acts by inhibiting the expression of <i>WUSCHEL</i> (<i>WUS</i>) but there is little research about how the receptor transmits the CLV3 signal to <i>WUS</i> and inhibits its expression. The CRISPR/Cas9 method was employed to edit the first exon of tomato <i>SlCLV3</i> in this study, leading to the functional deletion of <i>SlCLV3</i>. As a result, flowers with a high number of organs and fruits with a high number of locules were produced. We screened six candidate genes using the transcriptome of <i>clv3</i> mutants, analyzed expression variations in these genes between the cultivated allele and wild-type allele of <i>fas</i>, and showed that only <i>SlLET6</i> and <i>SlGIF1</i> (<i>GRF1-interacting factor 1</i>) were influenced by the <i>fas</i> locus. <i>SlLET6</i> overexpression resulted in an increase in flower carpels and fruit locules. These results suggest that <i>SlLET6</i> may be the downstream gene of <i>SlCLV3</i> regulating the number of carpels and fruit locules in tomato.https://www.mdpi.com/2311-7524/10/1/11<i>CLAVATA3</i>tomatolocule numberdownstream geneSlLET6 |
| spellingShingle | Yaofeng Zhang Huixian Sun Linlin Tian Yanxiu Miao Leiping Hou Meihua Sun Mingfang Qi Tianlai Li Transcriptome Analysis Identifies Downstream Genes of <i>CLAVATA3</i> in Tomato Horticulturae <i>CLAVATA3</i> tomato locule number downstream gene SlLET6 |
| title | Transcriptome Analysis Identifies Downstream Genes of <i>CLAVATA3</i> in Tomato |
| title_full | Transcriptome Analysis Identifies Downstream Genes of <i>CLAVATA3</i> in Tomato |
| title_fullStr | Transcriptome Analysis Identifies Downstream Genes of <i>CLAVATA3</i> in Tomato |
| title_full_unstemmed | Transcriptome Analysis Identifies Downstream Genes of <i>CLAVATA3</i> in Tomato |
| title_short | Transcriptome Analysis Identifies Downstream Genes of <i>CLAVATA3</i> in Tomato |
| title_sort | transcriptome analysis identifies downstream genes of i clavata3 i in tomato |
| topic | <i>CLAVATA3</i> tomato locule number downstream gene SlLET6 |
| url | https://www.mdpi.com/2311-7524/10/1/11 |
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