Advantages and Limitations of Current Imaging Techniques for Characterizing Liposome Morphology
There are currently a number of imaging techniques available for evaluating the morphology of liposomes and other nanoparticles, with each having its own advantages and disadvantages that should be considered when interpreting data. Controlling and validating the morphology of nanoparticles is of ke...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2018.00080/full |
| _version_ | 1828428353474920448 |
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| author | Annie-Louise Robson Paul C. Dastoor Jamie Flynn Jamie Flynn William Palmer William Palmer Antony Martin Antony Martin Doug W. Smith Ameha Woldu Susan Hua Susan Hua |
| author_facet | Annie-Louise Robson Paul C. Dastoor Jamie Flynn Jamie Flynn William Palmer William Palmer Antony Martin Antony Martin Doug W. Smith Ameha Woldu Susan Hua Susan Hua |
| author_sort | Annie-Louise Robson |
| collection | DOAJ |
| description | There are currently a number of imaging techniques available for evaluating the morphology of liposomes and other nanoparticles, with each having its own advantages and disadvantages that should be considered when interpreting data. Controlling and validating the morphology of nanoparticles is of key importance for the effective clinical translation of liposomal formulations. There are a number of physical characteristics of liposomes that determine their in vivo behavior, including size, surface characteristics, lamellarity, and homogeneity. Despite the great importance of the morphology of nanoparticles, it is generally not well-characterized and is difficult to control. Appropriate imaging techniques provide important details regarding the morphological characteristics of nanoparticles, and should be used in conjunction with other methods to assess physicochemical parameters. In this review, we will discuss the advantages and limitations of available imaging techniques used to evaluate liposomal formulations. |
| first_indexed | 2024-12-10T17:16:21Z |
| format | Article |
| id | doaj.art-07d5670724124c16988aba35eff8aa14 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-10T17:16:21Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-07d5670724124c16988aba35eff8aa142022-12-22T01:40:07ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-02-01910.3389/fphar.2018.00080328115Advantages and Limitations of Current Imaging Techniques for Characterizing Liposome MorphologyAnnie-Louise Robson0Paul C. Dastoor1Jamie Flynn2Jamie Flynn3William Palmer4William Palmer5Antony Martin6Antony Martin7Doug W. Smith8Ameha Woldu9Susan Hua10Susan Hua11School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, AustraliaCentre for Organic Electronics, University of Newcastle, Callaghan, NSW, AustraliaSchool of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, AustraliaHunter Medical Research Institute, New Lambton Heights, NSW, AustraliaHunter Medical Research Institute, New Lambton Heights, NSW, AustraliaSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, AustraliaHunter Medical Research Institute, New Lambton Heights, NSW, AustraliaSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, AustraliaSchool of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, AustraliaHunter Medical Research Institute, New Lambton Heights, NSW, AustraliaSchool of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, AustraliaHunter Medical Research Institute, New Lambton Heights, NSW, AustraliaThere are currently a number of imaging techniques available for evaluating the morphology of liposomes and other nanoparticles, with each having its own advantages and disadvantages that should be considered when interpreting data. Controlling and validating the morphology of nanoparticles is of key importance for the effective clinical translation of liposomal formulations. There are a number of physical characteristics of liposomes that determine their in vivo behavior, including size, surface characteristics, lamellarity, and homogeneity. Despite the great importance of the morphology of nanoparticles, it is generally not well-characterized and is difficult to control. Appropriate imaging techniques provide important details regarding the morphological characteristics of nanoparticles, and should be used in conjunction with other methods to assess physicochemical parameters. In this review, we will discuss the advantages and limitations of available imaging techniques used to evaluate liposomal formulations.http://journal.frontiersin.org/article/10.3389/fphar.2018.00080/fullliposomesmicroscopyimagingnanoparticleslight microscopyelectron microscopy |
| spellingShingle | Annie-Louise Robson Paul C. Dastoor Jamie Flynn Jamie Flynn William Palmer William Palmer Antony Martin Antony Martin Doug W. Smith Ameha Woldu Susan Hua Susan Hua Advantages and Limitations of Current Imaging Techniques for Characterizing Liposome Morphology Frontiers in Pharmacology liposomes microscopy imaging nanoparticles light microscopy electron microscopy |
| title | Advantages and Limitations of Current Imaging Techniques for Characterizing Liposome Morphology |
| title_full | Advantages and Limitations of Current Imaging Techniques for Characterizing Liposome Morphology |
| title_fullStr | Advantages and Limitations of Current Imaging Techniques for Characterizing Liposome Morphology |
| title_full_unstemmed | Advantages and Limitations of Current Imaging Techniques for Characterizing Liposome Morphology |
| title_short | Advantages and Limitations of Current Imaging Techniques for Characterizing Liposome Morphology |
| title_sort | advantages and limitations of current imaging techniques for characterizing liposome morphology |
| topic | liposomes microscopy imaging nanoparticles light microscopy electron microscopy |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2018.00080/full |
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