Effects of female gonadal hormones and LPS on depressive-like behavior in rats
Considerable evidence shows an association of depression with the immune system and emphasizes the importance of gender in the etiology of the disease and the response to inflammatory stimuli. We examined the influence of immune-challenged systems on depressive-like behavior in female rats...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2015-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500065M.pdf |
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| author | Mitić Miloš Lukić Iva Božović Natalija Đorđević Jelena Adžić Miroslav |
| author_facet | Mitić Miloš Lukić Iva Božović Natalija Đorđević Jelena Adžić Miroslav |
| author_sort | Mitić Miloš |
| collection | DOAJ |
| description | Considerable evidence shows an association of depression with the immune
system and emphasizes the importance of gender in the etiology of the disease
and the response to inflammatory stimuli. We examined the influence of
immune-challenged systems on depressive-like behavior in female rats in the
context of gonadal hormones. We used a neuroinflammatory model of depression
elicited by lipopolysaccharide (LPS) administration on naive and
ovariectomized (OVX) female rats, and examined the effects of estradiol (E2)
and/or progesterone (P4) replacement therapy on animal behavior, as assessed
by the forced swimming test (FST). We found that LPS and OVX increase
immobility in the FST, while LPS also decreased body weight in naive female
rats. Further, even though P4 application alone showed beneficial effects on
the behavioral profile (it reduced immobility and increased climbing),
supplementation of both hormones (E2 and P4) together to OVX rats failed to
do so. When OVX rats were exposed to LPS-induced immune challenge, neither
hormone individually nor their combination had any effect on immobility,
however, their joint supplementation increased climbing behavior. In
conclusion, our study confirmed that both LPS and OVX induced depressive-like
behavior in female rats. Furthermore, our results potentiate P4
supplementation in relieving the depressive-like symptomatology in OVX rats,
most likely through fine-tuning of different neurotransmitter systems. In the
context of an activated immune system, the application of E2 and/or P4 does
not provide any advantageous effects on depressive-like behavior. |
| first_indexed | 2024-04-14T05:38:06Z |
| format | Article |
| id | doaj.art-07d607170c434ddc81236c34adef1586 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-14T05:38:06Z |
| publishDate | 2015-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-07d607170c434ddc81236c34adef15862022-12-22T02:09:34ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392015-01-016731025103210.2298/ABS150130065M0354-46641500065MEffects of female gonadal hormones and LPS on depressive-like behavior in ratsMitić Miloš0Lukić Iva1Božović Natalija2Đorđević Jelena3Adžić Miroslav4VINČA Institute of Nuclear Sciences, Department of Molecular Biology and Endocrinology, BelgradeVINČA Institute of Nuclear Sciences, Department of Molecular Biology and Endocrinology, BelgradeVINČA Institute of Nuclear Sciences, Department of Molecular Biology and Endocrinology, BelgradeVINČA Institute of Nuclear Sciences, Department of Molecular Biology and Endocrinology, BelgradeVINČA Institute of Nuclear Sciences, Department of Molecular Biology and Endocrinology, BelgradeConsiderable evidence shows an association of depression with the immune system and emphasizes the importance of gender in the etiology of the disease and the response to inflammatory stimuli. We examined the influence of immune-challenged systems on depressive-like behavior in female rats in the context of gonadal hormones. We used a neuroinflammatory model of depression elicited by lipopolysaccharide (LPS) administration on naive and ovariectomized (OVX) female rats, and examined the effects of estradiol (E2) and/or progesterone (P4) replacement therapy on animal behavior, as assessed by the forced swimming test (FST). We found that LPS and OVX increase immobility in the FST, while LPS also decreased body weight in naive female rats. Further, even though P4 application alone showed beneficial effects on the behavioral profile (it reduced immobility and increased climbing), supplementation of both hormones (E2 and P4) together to OVX rats failed to do so. When OVX rats were exposed to LPS-induced immune challenge, neither hormone individually nor their combination had any effect on immobility, however, their joint supplementation increased climbing behavior. In conclusion, our study confirmed that both LPS and OVX induced depressive-like behavior in female rats. Furthermore, our results potentiate P4 supplementation in relieving the depressive-like symptomatology in OVX rats, most likely through fine-tuning of different neurotransmitter systems. In the context of an activated immune system, the application of E2 and/or P4 does not provide any advantageous effects on depressive-like behavior.http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500065M.pdfLPSbehaviorgonadal hormonesovariectomyforce swimming test |
| spellingShingle | Mitić Miloš Lukić Iva Božović Natalija Đorđević Jelena Adžić Miroslav Effects of female gonadal hormones and LPS on depressive-like behavior in rats Archives of Biological Sciences LPS behavior gonadal hormones ovariectomy force swimming test |
| title | Effects of female gonadal hormones and LPS on depressive-like behavior in rats |
| title_full | Effects of female gonadal hormones and LPS on depressive-like behavior in rats |
| title_fullStr | Effects of female gonadal hormones and LPS on depressive-like behavior in rats |
| title_full_unstemmed | Effects of female gonadal hormones and LPS on depressive-like behavior in rats |
| title_short | Effects of female gonadal hormones and LPS on depressive-like behavior in rats |
| title_sort | effects of female gonadal hormones and lps on depressive like behavior in rats |
| topic | LPS behavior gonadal hormones ovariectomy force swimming test |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500065M.pdf |
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