Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets
The phagosome microenvironment maintains enzyme activity and function. Here we compared the phagosomal pH of human neutrophils, monocytes, dendritic cells (DC), and monocyte-derived cells. An unexpected observation was the striking difference in phagosomal environment between the three monocytes sub...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-03-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.00188/full |
| _version_ | 1819207636675985408 |
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| author | Juliet R. Foote Amit A. Patel Simon Yona Anthony W. Segal |
| author_facet | Juliet R. Foote Amit A. Patel Simon Yona Anthony W. Segal |
| author_sort | Juliet R. Foote |
| collection | DOAJ |
| description | The phagosome microenvironment maintains enzyme activity and function. Here we compared the phagosomal pH of human neutrophils, monocytes, dendritic cells (DC), and monocyte-derived cells. An unexpected observation was the striking difference in phagosomal environment between the three monocytes subsets. Classical monocytes and neutrophils exhibited alkaline phagosomes, yet non-classical monocytes had more acidic phagosomes, while intermediate monocytes had a phenotype in-between. We next investigated the differences between primary naïve DC vs. in vitro monocyte-derived DC (MoDC) and established that both these cells had acidic phagosomal environments. Across all phagocytes, alkalinization was dependent upon the activity of the NADPH oxidase activity, demonstrated by the absence of NADPH oxidase from a patient with chronic granulomatous disease (CGD) or the use of a pharmacological inhibitor, diphenylene iodonium (DPI). Interestingly, MoDC stimulated with bacterial lipopolysaccharide had increased phagosomal pH. Overall, the increase in alkalinity within the phagosome was associated with increased oxidase activity. These data highlight the heterogeneous nature and potential function of phagocytic vacuoles within the family of mononuclear phagocytes. |
| first_indexed | 2024-12-23T05:26:39Z |
| format | Article |
| id | doaj.art-07d694d74ac24902beca50a34a73a080 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-23T05:26:39Z |
| publishDate | 2019-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-07d694d74ac24902beca50a34a73a0802022-12-21T17:58:35ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-03-011010.3389/fimmu.2019.00188426014Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte SubsetsJuliet R. FooteAmit A. PatelSimon YonaAnthony W. SegalThe phagosome microenvironment maintains enzyme activity and function. Here we compared the phagosomal pH of human neutrophils, monocytes, dendritic cells (DC), and monocyte-derived cells. An unexpected observation was the striking difference in phagosomal environment between the three monocytes subsets. Classical monocytes and neutrophils exhibited alkaline phagosomes, yet non-classical monocytes had more acidic phagosomes, while intermediate monocytes had a phenotype in-between. We next investigated the differences between primary naïve DC vs. in vitro monocyte-derived DC (MoDC) and established that both these cells had acidic phagosomal environments. Across all phagocytes, alkalinization was dependent upon the activity of the NADPH oxidase activity, demonstrated by the absence of NADPH oxidase from a patient with chronic granulomatous disease (CGD) or the use of a pharmacological inhibitor, diphenylene iodonium (DPI). Interestingly, MoDC stimulated with bacterial lipopolysaccharide had increased phagosomal pH. Overall, the increase in alkalinity within the phagosome was associated with increased oxidase activity. These data highlight the heterogeneous nature and potential function of phagocytic vacuoles within the family of mononuclear phagocytes.https://www.frontiersin.org/article/10.3389/fimmu.2019.00188/fullneutrophilmonocytemacrophagedendritic cellphagocytosispH |
| spellingShingle | Juliet R. Foote Amit A. Patel Simon Yona Anthony W. Segal Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets Frontiers in Immunology neutrophil monocyte macrophage dendritic cell phagocytosis pH |
| title | Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets |
| title_full | Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets |
| title_fullStr | Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets |
| title_full_unstemmed | Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets |
| title_short | Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets |
| title_sort | variations in the phagosomal environment of human neutrophils and mononuclear phagocyte subsets |
| topic | neutrophil monocyte macrophage dendritic cell phagocytosis pH |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.00188/full |
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