Human Antimicrobial Peptide Isolated From Triatoma infestans Haemolymph, Trypanosoma cruzi-Transmitting Vector
The importance of antimicrobial peptides (AMPs) in relation to the survival of invertebrates is well known. The source and the mode of action on the insects' immune system of these molecules have been described from different perspectives. Insects produce their own AMPs as well as obtain these...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2018.00354/full |
| _version_ | 1819075993315311616 |
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| author | Laura Cristina Lima Diniz Laura Cristina Lima Diniz Antonio Miranda Pedro Ismael da Silva Jr. |
| author_facet | Laura Cristina Lima Diniz Laura Cristina Lima Diniz Antonio Miranda Pedro Ismael da Silva Jr. |
| author_sort | Laura Cristina Lima Diniz |
| collection | DOAJ |
| description | The importance of antimicrobial peptides (AMPs) in relation to the survival of invertebrates is well known. The source and the mode of action on the insects' immune system of these molecules have been described from different perspectives. Insects produce their own AMPs as well as obtain these molecules from various sources, for example by absorption through the intestinal tract, as previously described for Boophilus microplus. Blood-sucking barber bug Triatoma infestans attracts social, economic and medical interest owing to its role in the transmission of Chagas disease. Despite new studies, descriptions of AMPs from this insect have remained elusive. Thus, the aims of this work were to characterize the antimicrobial potential of human fibrinopeptide A (FbPA) obtained from the T. infestans haemolymph and identify its natural source. Therefore, FbPA was isolated from the T. infestans haemolymph through liquid chromatography and identified by mass spectrometry. This peptide exhibited antimicrobial activity against Micrococcus luteus. Native FbPA from human blood and the synthetic FbPA also exhibited antimicrobial activity. The synthetic FbPA was conjugated with fluorescein isothiocyanate and offered to the insects. The haemolymph collected after 72 h exhibited fluorescence at the same wavelength as fluorescein isothiocyanate. Our experiments show that beyond intrinsic AMP production, T. infestans is able to co-opt molecules via internalization and may use them as AMPs for protection. |
| first_indexed | 2024-12-21T18:34:14Z |
| format | Article |
| id | doaj.art-07d957a6f64e4c8ca5e0d8bf3693e6d7 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-21T18:34:14Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-07d957a6f64e4c8ca5e0d8bf3693e6d72022-12-21T18:54:11ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882018-10-01810.3389/fcimb.2018.00354411964Human Antimicrobial Peptide Isolated From Triatoma infestans Haemolymph, Trypanosoma cruzi-Transmitting VectorLaura Cristina Lima Diniz0Laura Cristina Lima Diniz1Antonio Miranda2Pedro Ismael da Silva Jr.3Special Laboratory of Toxinology, Butantan Institute, São Paulo, BrazilPost-Graduation Program Interunits in Biotechnology, USP/IPT/IBU, São Paulo, BrazilDepartment of Biophysics, UNIFESP, São Paulo, BrazilSpecial Laboratory of Toxinology, Butantan Institute, São Paulo, BrazilThe importance of antimicrobial peptides (AMPs) in relation to the survival of invertebrates is well known. The source and the mode of action on the insects' immune system of these molecules have been described from different perspectives. Insects produce their own AMPs as well as obtain these molecules from various sources, for example by absorption through the intestinal tract, as previously described for Boophilus microplus. Blood-sucking barber bug Triatoma infestans attracts social, economic and medical interest owing to its role in the transmission of Chagas disease. Despite new studies, descriptions of AMPs from this insect have remained elusive. Thus, the aims of this work were to characterize the antimicrobial potential of human fibrinopeptide A (FbPA) obtained from the T. infestans haemolymph and identify its natural source. Therefore, FbPA was isolated from the T. infestans haemolymph through liquid chromatography and identified by mass spectrometry. This peptide exhibited antimicrobial activity against Micrococcus luteus. Native FbPA from human blood and the synthetic FbPA also exhibited antimicrobial activity. The synthetic FbPA was conjugated with fluorescein isothiocyanate and offered to the insects. The haemolymph collected after 72 h exhibited fluorescence at the same wavelength as fluorescein isothiocyanate. Our experiments show that beyond intrinsic AMP production, T. infestans is able to co-opt molecules via internalization and may use them as AMPs for protection.https://www.frontiersin.org/article/10.3389/fcimb.2018.00354/fullantimicrobial peptidesTriatoma infestansfibrinopeptide Ainnate immune systeminternalization |
| spellingShingle | Laura Cristina Lima Diniz Laura Cristina Lima Diniz Antonio Miranda Pedro Ismael da Silva Jr. Human Antimicrobial Peptide Isolated From Triatoma infestans Haemolymph, Trypanosoma cruzi-Transmitting Vector Frontiers in Cellular and Infection Microbiology antimicrobial peptides Triatoma infestans fibrinopeptide A innate immune system internalization |
| title | Human Antimicrobial Peptide Isolated From Triatoma infestans Haemolymph, Trypanosoma cruzi-Transmitting Vector |
| title_full | Human Antimicrobial Peptide Isolated From Triatoma infestans Haemolymph, Trypanosoma cruzi-Transmitting Vector |
| title_fullStr | Human Antimicrobial Peptide Isolated From Triatoma infestans Haemolymph, Trypanosoma cruzi-Transmitting Vector |
| title_full_unstemmed | Human Antimicrobial Peptide Isolated From Triatoma infestans Haemolymph, Trypanosoma cruzi-Transmitting Vector |
| title_short | Human Antimicrobial Peptide Isolated From Triatoma infestans Haemolymph, Trypanosoma cruzi-Transmitting Vector |
| title_sort | human antimicrobial peptide isolated from triatoma infestans haemolymph trypanosoma cruzi transmitting vector |
| topic | antimicrobial peptides Triatoma infestans fibrinopeptide A innate immune system internalization |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2018.00354/full |
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