Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms
Cellulosic ethanol has been gaining high attention due to its potential to reduce the greenhouse gas emission and cut down the world dependence on fossil fuels. Biorefinery approach for cellulosic ethanol has advantages due to its non-food competing status, natural abundance and benefit to decrease...
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| Format: | Article |
| Language: | English |
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Tamkang University Press
2023-08-01
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| Series: | Journal of Applied Science and Engineering |
| Subjects: | |
| Online Access: | http://jase.tku.edu.tw/articles/jase-202402-27-2-0001 |
| _version_ | 1828716157005201408 |
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| author | Malinee Sriariyanun Marttin Paulraj Gundupalli Vanarat Phakeenuya Theerawut Phusamtisampan Yu-Shen Cheng Ponnusami Venkatachalam |
| author_facet | Malinee Sriariyanun Marttin Paulraj Gundupalli Vanarat Phakeenuya Theerawut Phusamtisampan Yu-Shen Cheng Ponnusami Venkatachalam |
| author_sort | Malinee Sriariyanun |
| collection | DOAJ |
| description | Cellulosic ethanol has been gaining high attention due to its potential to reduce the greenhouse gas emission and cut down the world dependence on fossil fuels. Biorefinery approach for cellulosic ethanol has advantages due to its non-food competing status, natural abundance and benefit to decrease the combustion of agricultural wastes after harvesting seasons. Due to the recalcitrant structure of lignocellulose biomass, pretreatment and hydrolysis are critical to determine the economic viability of the process because they influence the conversion rate of fermentable sugars and, subsequently, final product i.e. ethanol. Therefore, the design for the process to compromise fermentation and upstream process is also essential. With all constraints exist when using harsh conditions during pretreatment, the recombinant engineered microorganisms have been developed and applied as biocatalysts during fermentation. To achieve the maximum production efficiency, different strategies of recombinant engineered microbes include expression optimization to modify the metabolic pathway, modification of secretion and transportation routes, improvement of stress tolerance, and utilization of both C5 and C6 sugars. This review provides the development and current status of cellulosic ethanol production via biorefining process by genetic engineered microbes with a focus on the technological aspects. The remaining challenges, perspective, and economical feasibility of the process are also discussed. |
| first_indexed | 2024-03-12T14:08:21Z |
| format | Article |
| id | doaj.art-07e1d05ced9249319d26f9ce4f3e9e3b |
| institution | Directory Open Access Journal |
| issn | 2708-9967 2708-9975 |
| language | English |
| last_indexed | 2024-03-12T14:08:21Z |
| publishDate | 2023-08-01 |
| publisher | Tamkang University Press |
| record_format | Article |
| series | Journal of Applied Science and Engineering |
| spelling | doaj.art-07e1d05ced9249319d26f9ce4f3e9e3b2023-08-21T09:17:21ZengTamkang University PressJournal of Applied Science and Engineering2708-99672708-99752023-08-012711985200510.6180/jase.202402_27(2).0001Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganismsMalinee Sriariyanun0Marttin Paulraj Gundupalli1Vanarat Phakeenuya2Theerawut Phusamtisampan3Yu-Shen Cheng4Ponnusami Venkatachalam5Biorefinery and Process Automation Engineering Center, Department of Chemical and Process Engineering, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok, ThailandBiorefinery and Process Automation Engineering Center, Department of Chemical and Process Engineering, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok, ThailandDepartment of Biotechnology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, ThailandDepartment of Biotechnology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, ThailandDepartment of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Douliu, Yunlin 64002, TaiwanBioprocess Intensification Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Thanjavur 613401, IndiaCellulosic ethanol has been gaining high attention due to its potential to reduce the greenhouse gas emission and cut down the world dependence on fossil fuels. Biorefinery approach for cellulosic ethanol has advantages due to its non-food competing status, natural abundance and benefit to decrease the combustion of agricultural wastes after harvesting seasons. Due to the recalcitrant structure of lignocellulose biomass, pretreatment and hydrolysis are critical to determine the economic viability of the process because they influence the conversion rate of fermentable sugars and, subsequently, final product i.e. ethanol. Therefore, the design for the process to compromise fermentation and upstream process is also essential. With all constraints exist when using harsh conditions during pretreatment, the recombinant engineered microorganisms have been developed and applied as biocatalysts during fermentation. To achieve the maximum production efficiency, different strategies of recombinant engineered microbes include expression optimization to modify the metabolic pathway, modification of secretion and transportation routes, improvement of stress tolerance, and utilization of both C5 and C6 sugars. This review provides the development and current status of cellulosic ethanol production via biorefining process by genetic engineered microbes with a focus on the technological aspects. The remaining challenges, perspective, and economical feasibility of the process are also discussed.http://jase.tku.edu.tw/articles/jase-202402-27-2-0001biorefineryenzymatic saccharificationethanolfermentationlignocellulosic biomasspretreatmentrecombinant engineering |
| spellingShingle | Malinee Sriariyanun Marttin Paulraj Gundupalli Vanarat Phakeenuya Theerawut Phusamtisampan Yu-Shen Cheng Ponnusami Venkatachalam Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms Journal of Applied Science and Engineering biorefinery enzymatic saccharification ethanol fermentation lignocellulosic biomass pretreatment recombinant engineering |
| title | Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms |
| title_full | Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms |
| title_fullStr | Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms |
| title_full_unstemmed | Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms |
| title_short | Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms |
| title_sort | biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms |
| topic | biorefinery enzymatic saccharification ethanol fermentation lignocellulosic biomass pretreatment recombinant engineering |
| url | http://jase.tku.edu.tw/articles/jase-202402-27-2-0001 |
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