Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique
Adrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope an...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1998-09-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900007 |
| _version_ | 1818036186614071296 |
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| author | R.C. Silva S. Faiçal S. Laureti A. Falorni S.A. Dib C.E. Kater |
| author_facet | R.C. Silva S. Faiçal S. Laureti A. Falorni S.A. Dib C.E. Kater |
| author_sort | R.C. Silva |
| collection | DOAJ |
| description | Adrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope and the fact that histological sections cannot be stored for long periods of time. To circumvent these restrictions we developed a novel peroxidase-labelled protein A (PLPA) technique for the detection of ACA in patients with Addison's disease and compared the results with those obtained with the classical IIF assay. We studied serum samples from 90 healthy control subjects and 22 patients with Addison's disease, who had been clinically classified into two groups: idiopathic (N = 13) and granulomatous (N = 9). ACA-PLPA were detected in 10/22 (45%) patients: 9/13 (69%) with the idiopathic form and 1/9 (11%) with the granulomatous form, whereas ACA-IIF were detected in 11/22 patients (50%): 10/13 (77%) with the idiopathic form and 1/9 (11%) with the granulomatous form. Twelve of the 13 idiopathic addisonians (92%) were positive for either ACA-PLPA or ACA-IIF, but only 7 were positive by both methods. In contrast, none of 90 healthy subjects was found to be positive for ACA. Thus, our study shows that the PLPA-based technique is useful, has technical advantages over the IIF method (by not requiring the use of a fluorescence microscope and by permitting section storage for long periods of time). However, since it is only 60% concordant with the ACA-IIF method, it should be considered complementary instead of an alternative method to IIF for the detection of ACA in human sera. |
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| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-10T07:06:57Z |
| publishDate | 1998-09-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-07e40e805eb74b2eb527652e53cce1f42022-12-22T01:58:10ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1998-09-01319114110.1590/S0100-879X1998000900007Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A techniqueR.C. SilvaS. FaiçalS. LauretiA. FalorniS.A. DibC.E. KaterAdrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope and the fact that histological sections cannot be stored for long periods of time. To circumvent these restrictions we developed a novel peroxidase-labelled protein A (PLPA) technique for the detection of ACA in patients with Addison's disease and compared the results with those obtained with the classical IIF assay. We studied serum samples from 90 healthy control subjects and 22 patients with Addison's disease, who had been clinically classified into two groups: idiopathic (N = 13) and granulomatous (N = 9). ACA-PLPA were detected in 10/22 (45%) patients: 9/13 (69%) with the idiopathic form and 1/9 (11%) with the granulomatous form, whereas ACA-IIF were detected in 11/22 patients (50%): 10/13 (77%) with the idiopathic form and 1/9 (11%) with the granulomatous form. Twelve of the 13 idiopathic addisonians (92%) were positive for either ACA-PLPA or ACA-IIF, but only 7 were positive by both methods. In contrast, none of 90 healthy subjects was found to be positive for ACA. Thus, our study shows that the PLPA-based technique is useful, has technical advantages over the IIF method (by not requiring the use of a fluorescence microscope and by permitting section storage for long periods of time). However, since it is only 60% concordant with the ACA-IIF method, it should be considered complementary instead of an alternative method to IIF for the detection of ACA in human sera.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900007autoantibodies detectionAddison's diseaseperoxidase-labelled protein Aindirect immunofluorescenceadrenal gland |
| spellingShingle | R.C. Silva S. Faiçal S. Laureti A. Falorni S.A. Dib C.E. Kater Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique Brazilian Journal of Medical and Biological Research autoantibodies detection Addison's disease peroxidase-labelled protein A indirect immunofluorescence adrenal gland |
| title | Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title_full | Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title_fullStr | Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title_full_unstemmed | Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title_short | Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique |
| title_sort | detection of adrenocortical autoantibodies in addison s disease with a peroxidase labelled protein a technique |
| topic | autoantibodies detection Addison's disease peroxidase-labelled protein A indirect immunofluorescence adrenal gland |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900007 |
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