Comparison of In Vitro Estrogenic Activity of <i>Polygoni multiflori</i> Radix and <i>Cynanchi wilfordii</i> Radix via the Enhancement of ERα/β Expression in MCF7 Cells
Postmenopausal women experience several symptoms, including inflammation and a sharp rise in oxidative stress caused by estrogen deprivation. Although estrogen replacement therapy (ERT) is generally regarded as an effective treatment for menopause, it has been used less frequently due to some advers...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/5/2199 |
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| author | Reshmi Akter Dong Uk Yang Jong Chan Ahn Muhammad Awais Jinnatun Nahar Zelika Mega Ramadhania Jong Yun Kim Gyong Jai Lee Gi-Young Kwak Dong Wook Lee Byoung Man Kong Deok Chun Yang Seok-Kyu Jung |
| author_facet | Reshmi Akter Dong Uk Yang Jong Chan Ahn Muhammad Awais Jinnatun Nahar Zelika Mega Ramadhania Jong Yun Kim Gyong Jai Lee Gi-Young Kwak Dong Wook Lee Byoung Man Kong Deok Chun Yang Seok-Kyu Jung |
| author_sort | Reshmi Akter |
| collection | DOAJ |
| description | Postmenopausal women experience several symptoms, including inflammation and a sharp rise in oxidative stress caused by estrogen deprivation. Although estrogen replacement therapy (ERT) is generally regarded as an effective treatment for menopause, it has been used less frequently due to some adverse effects and high costs. Therefore, there is an immediate need to develop an effective herbal-based treatment that is affordable for low-income populations. Acordingly, this study explored the estrogen-like properties of methanol extracts from <i>Cynanchum wilfordii</i> (CW) and <i>Poligonum multiflorum</i> (PM), two important medicinal plants in Republic of Korea, Japan, and China. Due to the similar names and morphologies of these two radixes, they are frequently confused in the marketplace. Our previous colleagues discriminated between these two plants. In this study, we investigated the estrogenic activity of PM and CW using several in vitro assays with their possible mechanism of action. First, their phytochemical contents, such as gallic acid, 2,3,5,4′-tetrahydroxystilbene-2-O-glucoside (TSG) and emodin, were quantified using high-performance liquid chromatography (HPLC). Secondly, estrogen-like activity was assessed utilizing the well-known E-screen test and gene expression analysis in estrogen receptor (ER)-positive MCF7 cells. ROS inhibition and anti-inflammatory effects were analyzed using HaCaT and Raw 264.7 cells, respectively. Our findings demonstrate that PM extracts significantly increased the expression of the estrogen-dependent genes (ERα, ERβ, pS2) and boosted MCF7 cell proliferation in comparison to CW extracts. Additionally, PM extract demonstrated a significant reduction in reactive oxygen species (ROS) production as well as an enhanced antioxidant profile compared to the CW extract. Further, the PM extract treatment significantly reduced the generation of nitric oxide (NO) in RAW 264.7 cells, a murine macrophage cell line, demonstrating the anti-inflammatory properties of the extract. Finally, this research offers an experimental foundation for the use of PM as a phytoestrogen to minimize menopausal symptoms. |
| first_indexed | 2024-03-11T07:17:13Z |
| format | Article |
| id | doaj.art-07e486da2b1944eeabc25f18e2c36583 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T07:17:13Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-07e486da2b1944eeabc25f18e2c365832023-11-17T08:13:21ZengMDPI AGMolecules1420-30492023-02-01285219910.3390/molecules28052199Comparison of In Vitro Estrogenic Activity of <i>Polygoni multiflori</i> Radix and <i>Cynanchi wilfordii</i> Radix via the Enhancement of ERα/β Expression in MCF7 CellsReshmi Akter0Dong Uk Yang1Jong Chan Ahn2Muhammad Awais3Jinnatun Nahar4Zelika Mega Ramadhania5Jong Yun Kim6Gyong Jai Lee7Gi-Young Kwak8Dong Wook Lee9Byoung Man Kong10Deok Chun Yang11Seok-Kyu Jung12Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of KoreaHanbangbio Inc., Yongin-si 17104, Gyeonggi-do, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of KoreaSaeromHanbang R&D Center, 76, Cheonseok-gil, Geumcheon-myeon, Naju-si 520010, Jeollanam-do, Republic of KoreaSD Leo R&D Center, 9-16, Yeonmujang 5-gil, Seongdong-gu, Seoul 100011, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of KoreaHanbangbio Inc., Yongin-si 17104, Gyeonggi-do, Republic of KoreaDepartment of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of KoreaGraduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of KoreaDepartment of Horticulture, Kongju National University, Yesan 32439, Republic of KoreaPostmenopausal women experience several symptoms, including inflammation and a sharp rise in oxidative stress caused by estrogen deprivation. Although estrogen replacement therapy (ERT) is generally regarded as an effective treatment for menopause, it has been used less frequently due to some adverse effects and high costs. Therefore, there is an immediate need to develop an effective herbal-based treatment that is affordable for low-income populations. Acordingly, this study explored the estrogen-like properties of methanol extracts from <i>Cynanchum wilfordii</i> (CW) and <i>Poligonum multiflorum</i> (PM), two important medicinal plants in Republic of Korea, Japan, and China. Due to the similar names and morphologies of these two radixes, they are frequently confused in the marketplace. Our previous colleagues discriminated between these two plants. In this study, we investigated the estrogenic activity of PM and CW using several in vitro assays with their possible mechanism of action. First, their phytochemical contents, such as gallic acid, 2,3,5,4′-tetrahydroxystilbene-2-O-glucoside (TSG) and emodin, were quantified using high-performance liquid chromatography (HPLC). Secondly, estrogen-like activity was assessed utilizing the well-known E-screen test and gene expression analysis in estrogen receptor (ER)-positive MCF7 cells. ROS inhibition and anti-inflammatory effects were analyzed using HaCaT and Raw 264.7 cells, respectively. Our findings demonstrate that PM extracts significantly increased the expression of the estrogen-dependent genes (ERα, ERβ, pS2) and boosted MCF7 cell proliferation in comparison to CW extracts. Additionally, PM extract demonstrated a significant reduction in reactive oxygen species (ROS) production as well as an enhanced antioxidant profile compared to the CW extract. Further, the PM extract treatment significantly reduced the generation of nitric oxide (NO) in RAW 264.7 cells, a murine macrophage cell line, demonstrating the anti-inflammatory properties of the extract. Finally, this research offers an experimental foundation for the use of PM as a phytoestrogen to minimize menopausal symptoms.https://www.mdpi.com/1420-3049/28/5/2199<i>Poligonum multiflorum</i><i>Cynanchum wilfordii</i>phytoestrogenmenopauseestrogenic-activityinflammation |
| spellingShingle | Reshmi Akter Dong Uk Yang Jong Chan Ahn Muhammad Awais Jinnatun Nahar Zelika Mega Ramadhania Jong Yun Kim Gyong Jai Lee Gi-Young Kwak Dong Wook Lee Byoung Man Kong Deok Chun Yang Seok-Kyu Jung Comparison of In Vitro Estrogenic Activity of <i>Polygoni multiflori</i> Radix and <i>Cynanchi wilfordii</i> Radix via the Enhancement of ERα/β Expression in MCF7 Cells Molecules <i>Poligonum multiflorum</i> <i>Cynanchum wilfordii</i> phytoestrogen menopause estrogenic-activity inflammation |
| title | Comparison of In Vitro Estrogenic Activity of <i>Polygoni multiflori</i> Radix and <i>Cynanchi wilfordii</i> Radix via the Enhancement of ERα/β Expression in MCF7 Cells |
| title_full | Comparison of In Vitro Estrogenic Activity of <i>Polygoni multiflori</i> Radix and <i>Cynanchi wilfordii</i> Radix via the Enhancement of ERα/β Expression in MCF7 Cells |
| title_fullStr | Comparison of In Vitro Estrogenic Activity of <i>Polygoni multiflori</i> Radix and <i>Cynanchi wilfordii</i> Radix via the Enhancement of ERα/β Expression in MCF7 Cells |
| title_full_unstemmed | Comparison of In Vitro Estrogenic Activity of <i>Polygoni multiflori</i> Radix and <i>Cynanchi wilfordii</i> Radix via the Enhancement of ERα/β Expression in MCF7 Cells |
| title_short | Comparison of In Vitro Estrogenic Activity of <i>Polygoni multiflori</i> Radix and <i>Cynanchi wilfordii</i> Radix via the Enhancement of ERα/β Expression in MCF7 Cells |
| title_sort | comparison of in vitro estrogenic activity of i polygoni multiflori i radix and i cynanchi wilfordii i radix via the enhancement of erα β expression in mcf7 cells |
| topic | <i>Poligonum multiflorum</i> <i>Cynanchum wilfordii</i> phytoestrogen menopause estrogenic-activity inflammation |
| url | https://www.mdpi.com/1420-3049/28/5/2199 |
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