MicroRNA-199b Deregulation Shows Oncogenic Properties and Promising Clinical Value as Circulating Marker in Locally Advanced Rectal Cancer Patients
The identification of robust prognostic markers still represents a need in locally advanced rectal cancer (LARC). MicroRNAs (miRs) have progressively emerged as promising circulating markers, overcoming some limitations that traditional biopsy comprises. Tissue miR-199b deregulation has been reporte...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/4/2203 |
| _version_ | 1797479289167282176 |
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| author | Andrea Santos Ion Cristóbal Jaime Rubio Cristina Caramés Melani Luque Marta Sanz-Alvarez Miriam Morales-Gallego Juan Madoz-Gúrpide Federico Rojo Jesús García-Foncillas |
| author_facet | Andrea Santos Ion Cristóbal Jaime Rubio Cristina Caramés Melani Luque Marta Sanz-Alvarez Miriam Morales-Gallego Juan Madoz-Gúrpide Federico Rojo Jesús García-Foncillas |
| author_sort | Andrea Santos |
| collection | DOAJ |
| description | The identification of robust prognostic markers still represents a need in locally advanced rectal cancer (LARC). MicroRNAs (miRs) have progressively emerged as promising circulating markers, overcoming some limitations that traditional biopsy comprises. Tissue miR-199b deregulation has been reported to predict outcome and response to neoadjuvant chemoradiotherapy (nCRT) in LARC, and was also found to be associated with disease progression in colorectal cancer. However, its biological and clinical relevance remains to be fully clarified. Thus, we observed here that miR-199b regulates cell migration, aggressiveness, and cell growth, and inhibits colonosphere formation and induces caspase-dependent apoptosis. Moreover, miR-199b expression was quantified by real-time PCR in plasma samples from LARC patients and its downregulation was observed in 22.7% of cases. This alteration was found to be associated with higher tumor size (<i>p</i> = 0.002) and pathological stage (<i>p</i> = 0.020) after nCRT. Notably, we observed substantially lower global miR-199b expression associated with patient downstaging (<i>p</i> = 0.009), as well as in non-responders compared to those cases who responded to nCRT in both pre- (<i>p</i> = 0.003) and post-treatment samples (<i>p</i> = 0.038). In concordance, we found that miR-199b served as a predictor marker of response to neoadjuvant therapy in our cohort (<i>p</i> = 0.011). Altogether, our findings here demonstrate the functional relevance of miR-199b in this disease and its potential value as a novel circulating marker in LARC. |
| first_indexed | 2024-03-09T21:43:42Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:43:42Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-07e4b92d14354682a84b34289bcb034a2023-11-23T20:21:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01234220310.3390/ijms23042203MicroRNA-199b Deregulation Shows Oncogenic Properties and Promising Clinical Value as Circulating Marker in Locally Advanced Rectal Cancer PatientsAndrea Santos0Ion Cristóbal1Jaime Rubio2Cristina Caramés3Melani Luque4Marta Sanz-Alvarez5Miriam Morales-Gallego6Juan Madoz-Gúrpide7Federico Rojo8Jesús García-Foncillas9Cancer Unit for Research on Novel Therapeutic Targets, Oncohealth Institute, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainCancer Unit for Research on Novel Therapeutic Targets, Oncohealth Institute, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainCancer Unit for Research on Novel Therapeutic Targets, Oncohealth Institute, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainCancer Unit for Research on Novel Therapeutic Targets, Oncohealth Institute, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainPathology Department, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainPathology Department, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainPathology Department, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainPathology Department, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainPathology Department, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainTranslational Oncology Division, Oncohealth Institute, IIS-Fundación Jiménez Díaz-UAM, 28040 Madrid, SpainThe identification of robust prognostic markers still represents a need in locally advanced rectal cancer (LARC). MicroRNAs (miRs) have progressively emerged as promising circulating markers, overcoming some limitations that traditional biopsy comprises. Tissue miR-199b deregulation has been reported to predict outcome and response to neoadjuvant chemoradiotherapy (nCRT) in LARC, and was also found to be associated with disease progression in colorectal cancer. However, its biological and clinical relevance remains to be fully clarified. Thus, we observed here that miR-199b regulates cell migration, aggressiveness, and cell growth, and inhibits colonosphere formation and induces caspase-dependent apoptosis. Moreover, miR-199b expression was quantified by real-time PCR in plasma samples from LARC patients and its downregulation was observed in 22.7% of cases. This alteration was found to be associated with higher tumor size (<i>p</i> = 0.002) and pathological stage (<i>p</i> = 0.020) after nCRT. Notably, we observed substantially lower global miR-199b expression associated with patient downstaging (<i>p</i> = 0.009), as well as in non-responders compared to those cases who responded to nCRT in both pre- (<i>p</i> = 0.003) and post-treatment samples (<i>p</i> = 0.038). In concordance, we found that miR-199b served as a predictor marker of response to neoadjuvant therapy in our cohort (<i>p</i> = 0.011). Altogether, our findings here demonstrate the functional relevance of miR-199b in this disease and its potential value as a novel circulating marker in LARC.https://www.mdpi.com/1422-0067/23/4/2203MiR-199btumor suppressorprognosisLARC |
| spellingShingle | Andrea Santos Ion Cristóbal Jaime Rubio Cristina Caramés Melani Luque Marta Sanz-Alvarez Miriam Morales-Gallego Juan Madoz-Gúrpide Federico Rojo Jesús García-Foncillas MicroRNA-199b Deregulation Shows Oncogenic Properties and Promising Clinical Value as Circulating Marker in Locally Advanced Rectal Cancer Patients International Journal of Molecular Sciences MiR-199b tumor suppressor prognosis LARC |
| title | MicroRNA-199b Deregulation Shows Oncogenic Properties and Promising Clinical Value as Circulating Marker in Locally Advanced Rectal Cancer Patients |
| title_full | MicroRNA-199b Deregulation Shows Oncogenic Properties and Promising Clinical Value as Circulating Marker in Locally Advanced Rectal Cancer Patients |
| title_fullStr | MicroRNA-199b Deregulation Shows Oncogenic Properties and Promising Clinical Value as Circulating Marker in Locally Advanced Rectal Cancer Patients |
| title_full_unstemmed | MicroRNA-199b Deregulation Shows Oncogenic Properties and Promising Clinical Value as Circulating Marker in Locally Advanced Rectal Cancer Patients |
| title_short | MicroRNA-199b Deregulation Shows Oncogenic Properties and Promising Clinical Value as Circulating Marker in Locally Advanced Rectal Cancer Patients |
| title_sort | microrna 199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients |
| topic | MiR-199b tumor suppressor prognosis LARC |
| url | https://www.mdpi.com/1422-0067/23/4/2203 |
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