Determinação espectrofotométrica de aspartame em adoçantes por injeção em fluxo usando um reator em fase sólida contendo fosfato de zinco imobilizado
A flow injection spectrophotometric method was developed for determining aspartame in sweeteners. Sample was dissolved in water and 250 µL of the solution was injected into a carrier stream of 5.0 x 10-5 mol L-1 sodium borate solution. The sample flowed through a column (14 cm x 2.0 mm) packed with...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2000-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422000000200005 |
| _version_ | 1818292363960778752 |
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| author | Pereira Airton Vicente Marcolino-Junior Luiz Humberto Fatibello-Filho Orlando |
| author_facet | Pereira Airton Vicente Marcolino-Junior Luiz Humberto Fatibello-Filho Orlando |
| author_sort | Pereira Airton Vicente |
| collection | DOAJ |
| description | A flow injection spectrophotometric method was developed for determining aspartame in sweeteners. Sample was dissolved in water and 250 µL of the solution was injected into a carrier stream of 5.0 x 10-5 mol L-1 sodium borate solution. The sample flowed through a column (14 cm x 2.0 mm) packed with Zn3(PO4)2 immobilized in a polymeric matrix of polyester resin and Zn(II) ions were released from the solid-phase reactor by formation of the Zn(II)-aspartame complex. The mixture merged with a stream of borate buffer solution (pH 9.0) containing 0.030 % (m/v) alizarin red S and the Zn(II)-alizarin red complex formed was measured spectrophotometrically at 540 nm. The calibration graph for aspartame was linear in the concentration range from 10 to 80 µg mL-1 with a detection limit of 4 µg mL-1 of aspartame. The RSD was 0.3 % for a solution containing 40 µg mL-1 aspartame (n = 10) and seventy results were obtained per hour. The proposed method was applied for determining aspartame in commercial sweeteners. |
| first_indexed | 2024-12-13T02:58:47Z |
| format | Article |
| id | doaj.art-07e642d68ce245498510b3cedf4fe240 |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-12-13T02:58:47Z |
| publishDate | 2000-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-07e642d68ce245498510b3cedf4fe2402022-12-22T00:01:54ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642000-01-01232167172Determinação espectrofotométrica de aspartame em adoçantes por injeção em fluxo usando um reator em fase sólida contendo fosfato de zinco imobilizadoPereira Airton VicenteMarcolino-Junior Luiz HumbertoFatibello-Filho OrlandoA flow injection spectrophotometric method was developed for determining aspartame in sweeteners. Sample was dissolved in water and 250 µL of the solution was injected into a carrier stream of 5.0 x 10-5 mol L-1 sodium borate solution. The sample flowed through a column (14 cm x 2.0 mm) packed with Zn3(PO4)2 immobilized in a polymeric matrix of polyester resin and Zn(II) ions were released from the solid-phase reactor by formation of the Zn(II)-aspartame complex. The mixture merged with a stream of borate buffer solution (pH 9.0) containing 0.030 % (m/v) alizarin red S and the Zn(II)-alizarin red complex formed was measured spectrophotometrically at 540 nm. The calibration graph for aspartame was linear in the concentration range from 10 to 80 µg mL-1 with a detection limit of 4 µg mL-1 of aspartame. The RSD was 0.3 % for a solution containing 40 µg mL-1 aspartame (n = 10) and seventy results were obtained per hour. The proposed method was applied for determining aspartame in commercial sweeteners.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422000000200005aspartameflow injection spectrophotometrysolid-phase reactorsweeteners |
| spellingShingle | Pereira Airton Vicente Marcolino-Junior Luiz Humberto Fatibello-Filho Orlando Determinação espectrofotométrica de aspartame em adoçantes por injeção em fluxo usando um reator em fase sólida contendo fosfato de zinco imobilizado Química Nova aspartame flow injection spectrophotometry solid-phase reactor sweeteners |
| title | Determinação espectrofotométrica de aspartame em adoçantes por injeção em fluxo usando um reator em fase sólida contendo fosfato de zinco imobilizado |
| title_full | Determinação espectrofotométrica de aspartame em adoçantes por injeção em fluxo usando um reator em fase sólida contendo fosfato de zinco imobilizado |
| title_fullStr | Determinação espectrofotométrica de aspartame em adoçantes por injeção em fluxo usando um reator em fase sólida contendo fosfato de zinco imobilizado |
| title_full_unstemmed | Determinação espectrofotométrica de aspartame em adoçantes por injeção em fluxo usando um reator em fase sólida contendo fosfato de zinco imobilizado |
| title_short | Determinação espectrofotométrica de aspartame em adoçantes por injeção em fluxo usando um reator em fase sólida contendo fosfato de zinco imobilizado |
| title_sort | determinacao espectrofotometrica de aspartame em adocantes por injecao em fluxo usando um reator em fase solida contendo fosfato de zinco imobilizado |
| topic | aspartame flow injection spectrophotometry solid-phase reactor sweeteners |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422000000200005 |
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