The regulation of MS-KIF18A expression and cross talk with estrogen receptor.
This study provides a novel view on the interactions between the MS-KIF18A, a kinesin protein, and estrogen receptor alpha (ERalpha) which were studied in vivo and in vitro. Additionally, the regulation of MS-KIF18A expression by estrogen was investigated at the gene and protein levels. An associati...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-07-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2712070?pdf=render |
| _version_ | 1819177721829261312 |
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| author | Margalit Zusev Dafna Benayahu |
| author_facet | Margalit Zusev Dafna Benayahu |
| author_sort | Margalit Zusev |
| collection | DOAJ |
| description | This study provides a novel view on the interactions between the MS-KIF18A, a kinesin protein, and estrogen receptor alpha (ERalpha) which were studied in vivo and in vitro. Additionally, the regulation of MS-KIF18A expression by estrogen was investigated at the gene and protein levels. An association between recombinant proteins; ERalpha and MS-KIF18A was demonstrated in vitro in a pull down assay. Such interactions were proven also for endogenous proteins in MBA-15 cells were detected prominently in the cytoplasm and are up-regulated by estrogen. Additionally, an association between these proteins and the transcription factor NF-kappaB was identified. MS-KIF18A mRNA expression was measured in vivo in relation to age and estrogen level in mice and rats models. A decrease in MS-KIF18A mRNA level was measured in old and in OVX-estrogen depleted rats as compared to young animals. The low MS-KIF18A mRNA expression in OVX rats was restored by estrogen treatment. We studied the regulation of MS-KIF18A transcription by estrogen using the luciferase reporter gene and chromatin immuno-precipitation (ChIP) assays. The luciferase reporter gene assay demonstrated an increase in MS-KIF18A promoter activity in response to 10(-8) M estrogen and 10(-7)M ICI-182,780. Complimentary, the ChIP assay quantified the binding of ERalpha and pcJun to the MS-KIF18A promoter that was enhanced in cells treated by estrogen and ICI-182,780. In addition, cells treated by estrogen expressed higher levels of MS-KIF18A mRNA and protein and the protein turnover in MBA-15 cells was accelerated. Presented data demonstrated that ERalpha is a defined cargo of MS-KIF18A and added novel insight on the role of estrogen in regulation of MS-KIF18A expression both in vivo and in vitro. |
| first_indexed | 2024-12-22T21:31:10Z |
| format | Article |
| id | doaj.art-07e685affc72417d8007b99b6de2eded |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T21:31:10Z |
| publishDate | 2009-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-07e685affc72417d8007b99b6de2eded2022-12-21T18:11:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-07-0147e640710.1371/journal.pone.0006407The regulation of MS-KIF18A expression and cross talk with estrogen receptor.Margalit ZusevDafna BenayahuThis study provides a novel view on the interactions between the MS-KIF18A, a kinesin protein, and estrogen receptor alpha (ERalpha) which were studied in vivo and in vitro. Additionally, the regulation of MS-KIF18A expression by estrogen was investigated at the gene and protein levels. An association between recombinant proteins; ERalpha and MS-KIF18A was demonstrated in vitro in a pull down assay. Such interactions were proven also for endogenous proteins in MBA-15 cells were detected prominently in the cytoplasm and are up-regulated by estrogen. Additionally, an association between these proteins and the transcription factor NF-kappaB was identified. MS-KIF18A mRNA expression was measured in vivo in relation to age and estrogen level in mice and rats models. A decrease in MS-KIF18A mRNA level was measured in old and in OVX-estrogen depleted rats as compared to young animals. The low MS-KIF18A mRNA expression in OVX rats was restored by estrogen treatment. We studied the regulation of MS-KIF18A transcription by estrogen using the luciferase reporter gene and chromatin immuno-precipitation (ChIP) assays. The luciferase reporter gene assay demonstrated an increase in MS-KIF18A promoter activity in response to 10(-8) M estrogen and 10(-7)M ICI-182,780. Complimentary, the ChIP assay quantified the binding of ERalpha and pcJun to the MS-KIF18A promoter that was enhanced in cells treated by estrogen and ICI-182,780. In addition, cells treated by estrogen expressed higher levels of MS-KIF18A mRNA and protein and the protein turnover in MBA-15 cells was accelerated. Presented data demonstrated that ERalpha is a defined cargo of MS-KIF18A and added novel insight on the role of estrogen in regulation of MS-KIF18A expression both in vivo and in vitro.http://europepmc.org/articles/PMC2712070?pdf=render |
| spellingShingle | Margalit Zusev Dafna Benayahu The regulation of MS-KIF18A expression and cross talk with estrogen receptor. PLoS ONE |
| title | The regulation of MS-KIF18A expression and cross talk with estrogen receptor. |
| title_full | The regulation of MS-KIF18A expression and cross talk with estrogen receptor. |
| title_fullStr | The regulation of MS-KIF18A expression and cross talk with estrogen receptor. |
| title_full_unstemmed | The regulation of MS-KIF18A expression and cross talk with estrogen receptor. |
| title_short | The regulation of MS-KIF18A expression and cross talk with estrogen receptor. |
| title_sort | regulation of ms kif18a expression and cross talk with estrogen receptor |
| url | http://europepmc.org/articles/PMC2712070?pdf=render |
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