BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen Activity
Small peptides secreted to the extracellular matrix control many aspects of the plant’s physiological activities which were identified in Arabidopsis thaliana, called ATSPs. Here, we isolated and characterized the small peptide gene Bna.SP6 from Brassica napus. The BnaC.SP6 promoter was cloned and i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.02117/full |
| _version_ | 1811233471201280000 |
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| author | Xuanpeng Wang Xin Li Mengmeng Li Jing Wen Bin Yi Jinxiong Shen Chaozhi Ma Tingdong Fu Jinxing Tu |
| author_facet | Xuanpeng Wang Xin Li Mengmeng Li Jing Wen Bin Yi Jinxiong Shen Chaozhi Ma Tingdong Fu Jinxing Tu |
| author_sort | Xuanpeng Wang |
| collection | DOAJ |
| description | Small peptides secreted to the extracellular matrix control many aspects of the plant’s physiological activities which were identified in Arabidopsis thaliana, called ATSPs. Here, we isolated and characterized the small peptide gene Bna.SP6 from Brassica napus. The BnaC.SP6 promoter was cloned and identified. Promoter deletion analysis suggested that the -447 to -375 and -210 to -135 regions are crucial for the silique septum and pollen expression of BnaC.SP6, respectively. Furthermore, the minimal promoter region of p158 (-210 to -52) was sufficient for driving gene expression specifically in pollen and highly conserved in Brassica species. In addition, BnaA.bZIP1 was predominantly expressed in anthers where BnaC.SP6 was also expressed, and was localized to the nuclei. BnaA.bZIP1 possessed transcriptional activation activity in yeast and protoplast system. It could specifically bind to the C-box in p158 in vitro, and negatively regulate p158 activity in vivo. BnaA.bZIP1 functions as a transcriptional repressor of BnaC.SP6 in pollen activity. These results provide novel insight into the transcriptional regulation of BnaC.SP6 in pollen activity and the pollen/anther-specific promoter regions of BnaC.SP6 may have their potential agricultural application for new male sterility line generation. |
| first_indexed | 2024-04-12T11:20:41Z |
| format | Article |
| id | doaj.art-07e8397e7e8f432f9bc94d63645c1955 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T11:20:41Z |
| publishDate | 2017-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-07e8397e7e8f432f9bc94d63645c19552022-12-22T03:35:22ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-12-01810.3389/fpls.2017.02117317484BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen ActivityXuanpeng WangXin LiMengmeng LiJing WenBin YiJinxiong ShenChaozhi MaTingdong FuJinxing TuSmall peptides secreted to the extracellular matrix control many aspects of the plant’s physiological activities which were identified in Arabidopsis thaliana, called ATSPs. Here, we isolated and characterized the small peptide gene Bna.SP6 from Brassica napus. The BnaC.SP6 promoter was cloned and identified. Promoter deletion analysis suggested that the -447 to -375 and -210 to -135 regions are crucial for the silique septum and pollen expression of BnaC.SP6, respectively. Furthermore, the minimal promoter region of p158 (-210 to -52) was sufficient for driving gene expression specifically in pollen and highly conserved in Brassica species. In addition, BnaA.bZIP1 was predominantly expressed in anthers where BnaC.SP6 was also expressed, and was localized to the nuclei. BnaA.bZIP1 possessed transcriptional activation activity in yeast and protoplast system. It could specifically bind to the C-box in p158 in vitro, and negatively regulate p158 activity in vivo. BnaA.bZIP1 functions as a transcriptional repressor of BnaC.SP6 in pollen activity. These results provide novel insight into the transcriptional regulation of BnaC.SP6 in pollen activity and the pollen/anther-specific promoter regions of BnaC.SP6 may have their potential agricultural application for new male sterility line generation.http://journal.frontiersin.org/article/10.3389/fpls.2017.02117/fullBrassica napussmall peptideBnaC.SP6pollen-specificBnaA.bZIP1transcriptional repressor |
| spellingShingle | Xuanpeng Wang Xin Li Mengmeng Li Jing Wen Bin Yi Jinxiong Shen Chaozhi Ma Tingdong Fu Jinxing Tu BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen Activity Frontiers in Plant Science Brassica napus small peptide BnaC.SP6 pollen-specific BnaA.bZIP1 transcriptional repressor |
| title | BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen Activity |
| title_full | BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen Activity |
| title_fullStr | BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen Activity |
| title_full_unstemmed | BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen Activity |
| title_short | BnaA.bZIP1 Negatively Regulates a Novel Small Peptide Gene, BnaC.SP6, Involved in Pollen Activity |
| title_sort | bnaa bzip1 negatively regulates a novel small peptide gene bnac sp6 involved in pollen activity |
| topic | Brassica napus small peptide BnaC.SP6 pollen-specific BnaA.bZIP1 transcriptional repressor |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.02117/full |
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