Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum
IntroductionThis study aimed to investigate the structure characterization and antiinflammatory activity of a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. and Hemsl.MethodsWe isolated a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. a...
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| Format: | Article |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1156798/full |
| _version_ | 1827979249929158656 |
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| author | Zhen Wang Hui Liu Ranze Fu Jinmei Ou Bin Wang |
| author_facet | Zhen Wang Hui Liu Ranze Fu Jinmei Ou Bin Wang |
| author_sort | Zhen Wang |
| collection | DOAJ |
| description | IntroductionThis study aimed to investigate the structure characterization and antiinflammatory activity of a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. and Hemsl.MethodsWe isolated a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. and Hemsl. for the first time, which was then successively purified through hot-water extraction, 80% alcohol precipitation, anion exchange and gel permeation chromatography. The in vitro anti-inflammatory activity of PKP2-1 in MH7A cells was assessed using a CCK-8 kit assay.ResultsMonosaccharide composition assay revealed that PKP2-1 was mainly composed of glucose, galactose, mannose, and glucuronic acid at an approximate molar ratio of 6:2:2:1. It had a molecular weight of approximately 17.34 kDa. Structural investigation revealed that the backbone of PKP2-1 consisted of (→2, 3)-α-D-Galp(4→, →2)-α-D-Manp(3→, →2)-β-D-Glcp(4→) and α-D-Glcp(3→) residues with side chains (→2)-β-D-Glcp(4→, →1)-α-D-Galp(4→) and α-D-Glcp(3→) branches located at O-3 position of (→2, 3)-α-D-Galp(4→). The in vitro anti-inflammatory activity of PKP2-1 in MH7A cells revealed that PKP2-1 could reduce the expression of IL-11β and IL-6, increase the expression of IL-10 and induce apoptosis of synovial fibroblasts.ConclusionThe PKP2-1 could inhibit MH7A cell growth and potentially be exploited as an anti-inflammatory agent. |
| first_indexed | 2024-04-09T21:34:18Z |
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| id | doaj.art-07e8e89504b24a0f8e3998c3f7ca5091 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-09T21:34:18Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-07e8e89504b24a0f8e3998c3f7ca50912023-03-27T04:38:47ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-03-011010.3389/fnut.2023.11567981156798Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianumZhen WangHui LiuRanze FuJinmei OuBin WangIntroductionThis study aimed to investigate the structure characterization and antiinflammatory activity of a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. and Hemsl.MethodsWe isolated a novel polysaccharide, PKP2-1, from the rhizomes of Polygonatum kingianum Coll. and Hemsl. for the first time, which was then successively purified through hot-water extraction, 80% alcohol precipitation, anion exchange and gel permeation chromatography. The in vitro anti-inflammatory activity of PKP2-1 in MH7A cells was assessed using a CCK-8 kit assay.ResultsMonosaccharide composition assay revealed that PKP2-1 was mainly composed of glucose, galactose, mannose, and glucuronic acid at an approximate molar ratio of 6:2:2:1. It had a molecular weight of approximately 17.34 kDa. Structural investigation revealed that the backbone of PKP2-1 consisted of (→2, 3)-α-D-Galp(4→, →2)-α-D-Manp(3→, →2)-β-D-Glcp(4→) and α-D-Glcp(3→) residues with side chains (→2)-β-D-Glcp(4→, →1)-α-D-Galp(4→) and α-D-Glcp(3→) branches located at O-3 position of (→2, 3)-α-D-Galp(4→). The in vitro anti-inflammatory activity of PKP2-1 in MH7A cells revealed that PKP2-1 could reduce the expression of IL-11β and IL-6, increase the expression of IL-10 and induce apoptosis of synovial fibroblasts.ConclusionThe PKP2-1 could inhibit MH7A cell growth and potentially be exploited as an anti-inflammatory agent.https://www.frontiersin.org/articles/10.3389/fnut.2023.1156798/fullpolysaccharideP. kingianumstructural elucidationanti-inflammatory activityMH7A cell |
| spellingShingle | Zhen Wang Hui Liu Ranze Fu Jinmei Ou Bin Wang Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum Frontiers in Nutrition polysaccharide P. kingianum structural elucidation anti-inflammatory activity MH7A cell |
| title | Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum |
| title_full | Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum |
| title_fullStr | Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum |
| title_full_unstemmed | Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum |
| title_short | Structural characterization and anti-inflammatory activity of a novel polysaccharide PKP2-1 from Polygonatum kingianum |
| title_sort | structural characterization and anti inflammatory activity of a novel polysaccharide pkp2 1 from polygonatum kingianum |
| topic | polysaccharide P. kingianum structural elucidation anti-inflammatory activity MH7A cell |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1156798/full |
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