RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi
Abstract Gibberellic acid (GA3) is a plant growth hormone that plays an important role in the production of crops, fruits, and vegetables with a wide market share. Due to intrinsic advantages, liquid fermentation of Fusarium fujikuroi has become the sole method for industrial GA3 production, but the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-10-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-022-00595-3 |
| _version_ | 1827970623311183872 |
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| author | Ya-Wen Li Cai-Ling Yang Hui Peng Zhi-Kui Nie Tian-Qiong Shi He Huang |
| author_facet | Ya-Wen Li Cai-Ling Yang Hui Peng Zhi-Kui Nie Tian-Qiong Shi He Huang |
| author_sort | Ya-Wen Li |
| collection | DOAJ |
| description | Abstract Gibberellic acid (GA3) is a plant growth hormone that plays an important role in the production of crops, fruits, and vegetables with a wide market share. Due to intrinsic advantages, liquid fermentation of Fusarium fujikuroi has become the sole method for industrial GA3 production, but the broader application of GA3 is hindered by low titer. In this study, we combined atmospheric and room-temperature plasma (ARTP) with ketoconazole-based screening to obtain the mutant strain 3-6-1 with high yield of GA3. Subsequently, the medium composition and fermentation parameters were systematically optimized to increase the titer of GA3, resulting in a 2.5-fold increase compared with the titer obtained under the initial conditions. Finally, considering that the strain is prone to substrate inhibition and glucose repression, a new strategy of fed-batch fermentation was adopted to increase the titer of GA3 to 575.13 mg/L, which was 13.86% higher than the control. The strategy of random mutagenesis combined with selection and fermentation optimization developed in this study provides a basis for subsequent research on the industrial production of GA3. Graphical Abstract |
| first_indexed | 2024-04-09T18:56:41Z |
| format | Article |
| id | doaj.art-07e9f149b92b476295df642d13322720 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-04-09T18:56:41Z |
| publishDate | 2022-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-07e9f149b92b476295df642d133227202023-04-09T11:06:52ZengSpringerOpenBioresources and Bioprocessing2197-43652022-10-019111510.1186/s40643-022-00595-3RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroiYa-Wen Li0Cai-Ling Yang1Hui Peng2Zhi-Kui Nie3Tian-Qiong Shi4He Huang5School of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversityCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityJiangxi New Reyphon Biochemical Co., Ltd., Salt and Chemical IndustrySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversityAbstract Gibberellic acid (GA3) is a plant growth hormone that plays an important role in the production of crops, fruits, and vegetables with a wide market share. Due to intrinsic advantages, liquid fermentation of Fusarium fujikuroi has become the sole method for industrial GA3 production, but the broader application of GA3 is hindered by low titer. In this study, we combined atmospheric and room-temperature plasma (ARTP) with ketoconazole-based screening to obtain the mutant strain 3-6-1 with high yield of GA3. Subsequently, the medium composition and fermentation parameters were systematically optimized to increase the titer of GA3, resulting in a 2.5-fold increase compared with the titer obtained under the initial conditions. Finally, considering that the strain is prone to substrate inhibition and glucose repression, a new strategy of fed-batch fermentation was adopted to increase the titer of GA3 to 575.13 mg/L, which was 13.86% higher than the control. The strategy of random mutagenesis combined with selection and fermentation optimization developed in this study provides a basis for subsequent research on the industrial production of GA3. Graphical Abstracthttps://doi.org/10.1186/s40643-022-00595-3ARTP mutagenesisFermentation optimizationFusarium fujikuroiGibberellic acidPlant growth hormone |
| spellingShingle | Ya-Wen Li Cai-Ling Yang Hui Peng Zhi-Kui Nie Tian-Qiong Shi He Huang RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi Bioresources and Bioprocessing ARTP mutagenesis Fermentation optimization Fusarium fujikuroi Gibberellic acid Plant growth hormone |
| title | RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi |
| title_full | RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi |
| title_fullStr | RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi |
| title_full_unstemmed | RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi |
| title_short | RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi |
| title_sort | retracted article mutagenesis combined with fermentation optimization to enhance gibberellic acid ga3 yield in fusarium fujikuroi |
| topic | ARTP mutagenesis Fermentation optimization Fusarium fujikuroi Gibberellic acid Plant growth hormone |
| url | https://doi.org/10.1186/s40643-022-00595-3 |
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