Oral antibiotics perturbation on gut microbiota after prostate biopsy

IntroductionThe use of antibiotics may induce the changes in gut microbiota. Previous studies have shown conflicting results on whether the changed gut microbiota by antibiotics can be recovered. Our study aims to investigate whether the gut microbiota could be recovered after a single dose of oral...

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Main Authors: Joseph Kai Man Li, Lynn Lin Wang, Becky Su Yan Lau, Ryan Tsz Hei Tse, Carol Ka Lo Cheng, Steven Chi Ho Leung, Christine Yim Ping Wong, Stephen Kwok Wing Tsui, Jeremy Yuen Chun Teoh, Peter Ka Fung Chiu, Chi Fai Ng
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-08-01
Series:Frontiers in Cellular and Infection Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2022.959903/full
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author Joseph Kai Man Li
Lynn Lin Wang
Becky Su Yan Lau
Ryan Tsz Hei Tse
Carol Ka Lo Cheng
Steven Chi Ho Leung
Christine Yim Ping Wong
Stephen Kwok Wing Tsui
Jeremy Yuen Chun Teoh
Peter Ka Fung Chiu
Chi Fai Ng
author_facet Joseph Kai Man Li
Lynn Lin Wang
Becky Su Yan Lau
Ryan Tsz Hei Tse
Carol Ka Lo Cheng
Steven Chi Ho Leung
Christine Yim Ping Wong
Stephen Kwok Wing Tsui
Jeremy Yuen Chun Teoh
Peter Ka Fung Chiu
Chi Fai Ng
author_sort Joseph Kai Man Li
collection DOAJ
description IntroductionThe use of antibiotics may induce the changes in gut microbiota. Previous studies have shown conflicting results on whether the changed gut microbiota by antibiotics can be recovered. Our study aims to investigate whether the gut microbiota could be recovered after a single dose of oral co-amoxiclav before transrectal ultrasound-guided transperineal prostate biopsy (TPPBx) in 5 weeks’ time.MethodsFifteen patients with elevated serum prostate-specific antigen (PSA) were recruited to provide pre-antibiotic and post-antibiotic fecal samples. The V4 region of 16S rRNA was sequenced. Analysis was performed by QIIME2. Alpha- and beta-diversities were analyzed, as well as the differential enrichment by Linear discriminant analysis Effect Size (LEfSe) analysis.ResultsBoth the alpha- and beta-diversities of the pre- and post-antibiotic fecal samples were significantly different. Genera that are associated with alleviation of inflammation were enriched in the pre-antibiotic fecal samples, while the inflammation-associated genera were more enriched in the post-antibiotic fecal samples.ConclusionA single dose of oral co-amoxiclav before TPPBx could have led to a change of gut microbiota that cannot be recovered in 5 weeks' time. Microbiome studies on prostate cancer patients should be cautioned on the use of post-prostate biopsy fecal sampling. Further studies should be conducted for the impact on gut microbiome for TPPBx alone.
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spelling doaj.art-07ec33b76aad4e3682020d36a0e372c22022-12-22T01:41:49ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-08-011210.3389/fcimb.2022.959903959903Oral antibiotics perturbation on gut microbiota after prostate biopsyJoseph Kai Man Li0Lynn Lin Wang1Becky Su Yan Lau2Ryan Tsz Hei Tse3Carol Ka Lo Cheng4Steven Chi Ho Leung5Christine Yim Ping Wong6Stephen Kwok Wing Tsui7Jeremy Yuen Chun Teoh8Peter Ka Fung Chiu9Chi Fai Ng10S.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaMetabolic Disease Research Centre, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaSchool of Biomedical Science, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaIntroductionThe use of antibiotics may induce the changes in gut microbiota. Previous studies have shown conflicting results on whether the changed gut microbiota by antibiotics can be recovered. Our study aims to investigate whether the gut microbiota could be recovered after a single dose of oral co-amoxiclav before transrectal ultrasound-guided transperineal prostate biopsy (TPPBx) in 5 weeks’ time.MethodsFifteen patients with elevated serum prostate-specific antigen (PSA) were recruited to provide pre-antibiotic and post-antibiotic fecal samples. The V4 region of 16S rRNA was sequenced. Analysis was performed by QIIME2. Alpha- and beta-diversities were analyzed, as well as the differential enrichment by Linear discriminant analysis Effect Size (LEfSe) analysis.ResultsBoth the alpha- and beta-diversities of the pre- and post-antibiotic fecal samples were significantly different. Genera that are associated with alleviation of inflammation were enriched in the pre-antibiotic fecal samples, while the inflammation-associated genera were more enriched in the post-antibiotic fecal samples.ConclusionA single dose of oral co-amoxiclav before TPPBx could have led to a change of gut microbiota that cannot be recovered in 5 weeks' time. Microbiome studies on prostate cancer patients should be cautioned on the use of post-prostate biopsy fecal sampling. Further studies should be conducted for the impact on gut microbiome for TPPBx alone.https://www.frontiersin.org/articles/10.3389/fcimb.2022.959903/fullantibioticsgut microbiotaprostate biopsybacteriafecal microbiota
spellingShingle Joseph Kai Man Li
Lynn Lin Wang
Becky Su Yan Lau
Ryan Tsz Hei Tse
Carol Ka Lo Cheng
Steven Chi Ho Leung
Christine Yim Ping Wong
Stephen Kwok Wing Tsui
Jeremy Yuen Chun Teoh
Peter Ka Fung Chiu
Chi Fai Ng
Oral antibiotics perturbation on gut microbiota after prostate biopsy
Frontiers in Cellular and Infection Microbiology
antibiotics
gut microbiota
prostate biopsy
bacteria
fecal microbiota
title Oral antibiotics perturbation on gut microbiota after prostate biopsy
title_full Oral antibiotics perturbation on gut microbiota after prostate biopsy
title_fullStr Oral antibiotics perturbation on gut microbiota after prostate biopsy
title_full_unstemmed Oral antibiotics perturbation on gut microbiota after prostate biopsy
title_short Oral antibiotics perturbation on gut microbiota after prostate biopsy
title_sort oral antibiotics perturbation on gut microbiota after prostate biopsy
topic antibiotics
gut microbiota
prostate biopsy
bacteria
fecal microbiota
url https://www.frontiersin.org/articles/10.3389/fcimb.2022.959903/full
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