Oral antibiotics perturbation on gut microbiota after prostate biopsy
IntroductionThe use of antibiotics may induce the changes in gut microbiota. Previous studies have shown conflicting results on whether the changed gut microbiota by antibiotics can be recovered. Our study aims to investigate whether the gut microbiota could be recovered after a single dose of oral...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2022-08-01
|
Series: | Frontiers in Cellular and Infection Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.959903/full |
_version_ | 1818486327099785216 |
---|---|
author | Joseph Kai Man Li Lynn Lin Wang Becky Su Yan Lau Ryan Tsz Hei Tse Carol Ka Lo Cheng Steven Chi Ho Leung Christine Yim Ping Wong Stephen Kwok Wing Tsui Jeremy Yuen Chun Teoh Peter Ka Fung Chiu Chi Fai Ng |
author_facet | Joseph Kai Man Li Lynn Lin Wang Becky Su Yan Lau Ryan Tsz Hei Tse Carol Ka Lo Cheng Steven Chi Ho Leung Christine Yim Ping Wong Stephen Kwok Wing Tsui Jeremy Yuen Chun Teoh Peter Ka Fung Chiu Chi Fai Ng |
author_sort | Joseph Kai Man Li |
collection | DOAJ |
description | IntroductionThe use of antibiotics may induce the changes in gut microbiota. Previous studies have shown conflicting results on whether the changed gut microbiota by antibiotics can be recovered. Our study aims to investigate whether the gut microbiota could be recovered after a single dose of oral co-amoxiclav before transrectal ultrasound-guided transperineal prostate biopsy (TPPBx) in 5 weeks’ time.MethodsFifteen patients with elevated serum prostate-specific antigen (PSA) were recruited to provide pre-antibiotic and post-antibiotic fecal samples. The V4 region of 16S rRNA was sequenced. Analysis was performed by QIIME2. Alpha- and beta-diversities were analyzed, as well as the differential enrichment by Linear discriminant analysis Effect Size (LEfSe) analysis.ResultsBoth the alpha- and beta-diversities of the pre- and post-antibiotic fecal samples were significantly different. Genera that are associated with alleviation of inflammation were enriched in the pre-antibiotic fecal samples, while the inflammation-associated genera were more enriched in the post-antibiotic fecal samples.ConclusionA single dose of oral co-amoxiclav before TPPBx could have led to a change of gut microbiota that cannot be recovered in 5 weeks' time. Microbiome studies on prostate cancer patients should be cautioned on the use of post-prostate biopsy fecal sampling. Further studies should be conducted for the impact on gut microbiome for TPPBx alone. |
first_indexed | 2024-12-10T16:21:28Z |
format | Article |
id | doaj.art-07ec33b76aad4e3682020d36a0e372c2 |
institution | Directory Open Access Journal |
issn | 2235-2988 |
language | English |
last_indexed | 2024-12-10T16:21:28Z |
publishDate | 2022-08-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Cellular and Infection Microbiology |
spelling | doaj.art-07ec33b76aad4e3682020d36a0e372c22022-12-22T01:41:49ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-08-011210.3389/fcimb.2022.959903959903Oral antibiotics perturbation on gut microbiota after prostate biopsyJoseph Kai Man Li0Lynn Lin Wang1Becky Su Yan Lau2Ryan Tsz Hei Tse3Carol Ka Lo Cheng4Steven Chi Ho Leung5Christine Yim Ping Wong6Stephen Kwok Wing Tsui7Jeremy Yuen Chun Teoh8Peter Ka Fung Chiu9Chi Fai Ng10S.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaMetabolic Disease Research Centre, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaSchool of Biomedical Science, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaS.H. Ho Urology Centre, Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaIntroductionThe use of antibiotics may induce the changes in gut microbiota. Previous studies have shown conflicting results on whether the changed gut microbiota by antibiotics can be recovered. Our study aims to investigate whether the gut microbiota could be recovered after a single dose of oral co-amoxiclav before transrectal ultrasound-guided transperineal prostate biopsy (TPPBx) in 5 weeks’ time.MethodsFifteen patients with elevated serum prostate-specific antigen (PSA) were recruited to provide pre-antibiotic and post-antibiotic fecal samples. The V4 region of 16S rRNA was sequenced. Analysis was performed by QIIME2. Alpha- and beta-diversities were analyzed, as well as the differential enrichment by Linear discriminant analysis Effect Size (LEfSe) analysis.ResultsBoth the alpha- and beta-diversities of the pre- and post-antibiotic fecal samples were significantly different. Genera that are associated with alleviation of inflammation were enriched in the pre-antibiotic fecal samples, while the inflammation-associated genera were more enriched in the post-antibiotic fecal samples.ConclusionA single dose of oral co-amoxiclav before TPPBx could have led to a change of gut microbiota that cannot be recovered in 5 weeks' time. Microbiome studies on prostate cancer patients should be cautioned on the use of post-prostate biopsy fecal sampling. Further studies should be conducted for the impact on gut microbiome for TPPBx alone.https://www.frontiersin.org/articles/10.3389/fcimb.2022.959903/fullantibioticsgut microbiotaprostate biopsybacteriafecal microbiota |
spellingShingle | Joseph Kai Man Li Lynn Lin Wang Becky Su Yan Lau Ryan Tsz Hei Tse Carol Ka Lo Cheng Steven Chi Ho Leung Christine Yim Ping Wong Stephen Kwok Wing Tsui Jeremy Yuen Chun Teoh Peter Ka Fung Chiu Chi Fai Ng Oral antibiotics perturbation on gut microbiota after prostate biopsy Frontiers in Cellular and Infection Microbiology antibiotics gut microbiota prostate biopsy bacteria fecal microbiota |
title | Oral antibiotics perturbation on gut microbiota after prostate biopsy |
title_full | Oral antibiotics perturbation on gut microbiota after prostate biopsy |
title_fullStr | Oral antibiotics perturbation on gut microbiota after prostate biopsy |
title_full_unstemmed | Oral antibiotics perturbation on gut microbiota after prostate biopsy |
title_short | Oral antibiotics perturbation on gut microbiota after prostate biopsy |
title_sort | oral antibiotics perturbation on gut microbiota after prostate biopsy |
topic | antibiotics gut microbiota prostate biopsy bacteria fecal microbiota |
url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.959903/full |
work_keys_str_mv | AT josephkaimanli oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT lynnlinwang oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT beckysuyanlau oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT ryantszheitse oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT carolkalocheng oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT stevenchiholeung oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT christineyimpingwong oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT stephenkwokwingtsui oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT jeremyyuenchunteoh oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT peterkafungchiu oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy AT chifaing oralantibioticsperturbationongutmicrobiotaafterprostatebiopsy |