Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders
Background: The automated hematology analyzers have replaced the traditional manual methods for assessing hematological parameters. Most of the automated hematology analyzers are programmed to identify abnormalities in the form of “suspect flags.” Aims: The aim of the study was to correlate white bl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Medical Journal of Dr. D.Y. Patil Vidyapeeth |
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| Online Access: | http://www.mjdrdypv.org/article.asp?issn=2589-8302;year=2020;volume=13;issue=6;spage=667;epage=671;aulast=Patro |
| _version_ | 1818944552510160896 |
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| author | Namrata Patro Archana C Buch Mukta D Naik Shruti Vimal Shirish S Chandanwale |
| author_facet | Namrata Patro Archana C Buch Mukta D Naik Shruti Vimal Shirish S Chandanwale |
| author_sort | Namrata Patro |
| collection | DOAJ |
| description | Background: The automated hematology analyzers have replaced the traditional manual methods for assessing hematological parameters. Most of the automated hematology analyzers are programmed to identify abnormalities in the form of “suspect flags.” Aims: The aim of the study was to correlate white blood cell (WBC) and platelet (PLT) flag messages provided by automated analyzers with their respective peripheral blood smear (PBS) findings. Materials and Methods: A descriptive cross-sectional study was undertaken on 100 patients who showed WBC and PLT suspect flags and/or abnormal peripheral smears. The flag messages were analyzed with their respective PBS findings. Pearson's Chi-square test was used for the statistical analysis. Results: Analyzer and PBS showed WBC defects (n = 46, 32), PLT defects (n = 39, 54), and combined defects (n = 36, 43) respectively. WBC defects included leukocytosis (n = 27, 22), leukopenia (n = 20, 18), immature granulocytes (n = 10, 3), left shift (n = 3, 2), myeloblast blast (n = 2, 1), and eosinophilia (n = 4, 4) on analyzer and PBS. Correlation between the WBC suspect flags and their peripheral smears was found to be statistically significant (P = 0.006). PLT defects included thrombocytopenia (n = 22, 30), thrombocytosis (n = 13, 17), giant PLTs (n = 10, 15), PLT clumps (n = 8, 12), and PLT debris (n = 2, 5) on analyzer and PBS. Correlation between the PLT characteristics in analyzers and in peripheral smears was statistically significant (P = 0.042). Conclusion: Suspect flags should be used as a screening tool to pick up pathological samples. These should then be followed up with a PBS examination for definitive diagnosis. |
| first_indexed | 2024-12-20T07:45:02Z |
| format | Article |
| id | doaj.art-07ed0882a3324135962b88aecfde527d |
| institution | Directory Open Access Journal |
| issn | 2589-8302 2589-8310 |
| language | English |
| last_indexed | 2024-12-20T07:45:02Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Dr. D.Y. Patil Vidyapeeth |
| spelling | doaj.art-07ed0882a3324135962b88aecfde527d2022-12-21T19:48:00ZengWolters Kluwer Medknow PublicationsMedical Journal of Dr. D.Y. Patil Vidyapeeth2589-83022589-83102020-01-0113666767110.4103/mjdrdypu.mjdrdypu_46_20Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disordersNamrata PatroArchana C BuchMukta D NaikShruti VimalShirish S ChandanwaleBackground: The automated hematology analyzers have replaced the traditional manual methods for assessing hematological parameters. Most of the automated hematology analyzers are programmed to identify abnormalities in the form of “suspect flags.” Aims: The aim of the study was to correlate white blood cell (WBC) and platelet (PLT) flag messages provided by automated analyzers with their respective peripheral blood smear (PBS) findings. Materials and Methods: A descriptive cross-sectional study was undertaken on 100 patients who showed WBC and PLT suspect flags and/or abnormal peripheral smears. The flag messages were analyzed with their respective PBS findings. Pearson's Chi-square test was used for the statistical analysis. Results: Analyzer and PBS showed WBC defects (n = 46, 32), PLT defects (n = 39, 54), and combined defects (n = 36, 43) respectively. WBC defects included leukocytosis (n = 27, 22), leukopenia (n = 20, 18), immature granulocytes (n = 10, 3), left shift (n = 3, 2), myeloblast blast (n = 2, 1), and eosinophilia (n = 4, 4) on analyzer and PBS. Correlation between the WBC suspect flags and their peripheral smears was found to be statistically significant (P = 0.006). PLT defects included thrombocytopenia (n = 22, 30), thrombocytosis (n = 13, 17), giant PLTs (n = 10, 15), PLT clumps (n = 8, 12), and PLT debris (n = 2, 5) on analyzer and PBS. Correlation between the PLT characteristics in analyzers and in peripheral smears was statistically significant (P = 0.042). Conclusion: Suspect flags should be used as a screening tool to pick up pathological samples. These should then be followed up with a PBS examination for definitive diagnosis.http://www.mjdrdypv.org/article.asp?issn=2589-8302;year=2020;volume=13;issue=6;spage=667;epage=671;aulast=Patroautomated analyzerflaggingperipheral blood smearplateletwhite blood cell |
| spellingShingle | Namrata Patro Archana C Buch Mukta D Naik Shruti Vimal Shirish S Chandanwale Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders Medical Journal of Dr. D.Y. Patil Vidyapeeth automated analyzer flagging peripheral blood smear platelet white blood cell |
| title | Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders |
| title_full | Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders |
| title_fullStr | Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders |
| title_full_unstemmed | Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders |
| title_short | Assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders |
| title_sort | assessment and reliability of suspect flags in automated hematology analyzers for diagnosing white blood cell and platelet disorders |
| topic | automated analyzer flagging peripheral blood smear platelet white blood cell |
| url | http://www.mjdrdypv.org/article.asp?issn=2589-8302;year=2020;volume=13;issue=6;spage=667;epage=671;aulast=Patro |
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