Cytotoxicity of Combination Doxorubicin and <i>Garcinia picrorrhiza</i> Fruit Extract on Fibroblast Cell
Combining chemotherapeutic agents such as doxorubicin with herbal products or other compounds that can enhance cytotoxicity without side effects is required. Thus, we aimed to observe the cytotoxicity of doxorubicin and sesoot (Garcinia picrorrhiza) fruit ethanolic extract (GpKar) on human fibroblas...
| المؤلفون الرئيسيون: | , , , , , , , , , |
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| التنسيق: | مقال |
| اللغة: | English |
| منشور في: |
Universitas Islam Bandung
2023-12-01
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| سلاسل: | Global Medical & Health Communication |
| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ejournal.unisba.ac.id/index.php/gmhc/article/view/10985 |
| _version_ | 1827379392671645696 |
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| author | Sri Utami Susi Endrini Lilian Batubara Nunung Ainur Rahmah Irfan Syarif Said Nafik Betharie Cendera Arrahmani Agung Novianto Hanna Sari Widya Kusuma Wahyu Widowati |
| author_facet | Sri Utami Susi Endrini Lilian Batubara Nunung Ainur Rahmah Irfan Syarif Said Nafik Betharie Cendera Arrahmani Agung Novianto Hanna Sari Widya Kusuma Wahyu Widowati |
| author_sort | Sri Utami |
| collection | DOAJ |
| description | Combining chemotherapeutic agents such as doxorubicin with herbal products or other compounds that can enhance cytotoxicity without side effects is required. Thus, we aimed to observe the cytotoxicity of doxorubicin and sesoot (Garcinia picrorrhiza) fruit ethanolic extract (GpKar) on human fibroblast cells, BJ. This study used a post-test-only control randomized group design with n=3 and a number group of 5. The method used in this research is cell number, and viability was measured with (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Treatments consisted of a combination of doxorubicin (0.02 μg/ml) and GpKar of 66.47 µg/ml (DES1), 132.94 µg/ml (DES2) and 265.89 μg/ml (DES3). The data were analyzed using a one-way ANOVA and Duncan post hoc tests. DES3 showed the lowest viability among treatments (89.32%). DES1 and DES2 showed high viability (>90%), 97.93%, and 95.08%, respectively. Thus, the combination of doxorubicin (0.02 μg/ml) and GpKar (66.47 µg/ml) was considered safe for further use in the following assay. In summary, the combination of doxorubicin and GpKar showed high viability in normal fibroblast cells. |
| first_indexed | 2024-03-08T13:15:57Z |
| format | Article |
| id | doaj.art-07ef07523a3c4b51a57c6dde237a8fb6 |
| institution | Directory Open Access Journal |
| issn | 2301-9123 2460-5441 |
| language | English |
| last_indexed | 2024-03-08T13:15:57Z |
| publishDate | 2023-12-01 |
| publisher | Universitas Islam Bandung |
| record_format | Article |
| series | Global Medical & Health Communication |
| spelling | doaj.art-07ef07523a3c4b51a57c6dde237a8fb62024-01-18T06:47:38ZengUniversitas Islam BandungGlobal Medical & Health Communication2301-91232460-54412023-12-0111310.29313/gmhc.v11i3.109854957Cytotoxicity of Combination Doxorubicin and <i>Garcinia picrorrhiza</i> Fruit Extract on Fibroblast CellSri Utami0Susi Endrini1Lilian Batubara2Nunung Ainur Rahmah3Irfan Syarif4Said Nafik5Betharie Cendera Arrahmani6Agung Novianto7Hanna Sari Widya Kusuma8Wahyu Widowati9Faculty of Medicine, Universitas YARSI, Central JakartaFaculty of Medicine, Universitas YARSI, Central JakartaFaculty of Medicine, Universitas YARSI, Central JakartaFaculty of Medicine, Universitas YARSI, Central JakartaFaculty of Medicine, Universitas YARSI, Central JakartaDirectorate General of Intellectual Property, Ministry of Law and Human Rights Republic of Indonesia, South JakartaRisk and Regulatory Consulting Pharma and Life Sciences, PricewaterhouseCoopers (PwC) GmbH WPG, FrankfurtBiomolecular and Biomedical Research Center, Aretha Medika Utama, BandungBiomolecular and Biomedical Research Center, Aretha Medika Utama, BandungFaculty of Medicine, Universitas Kristen Maranatha, BandungCombining chemotherapeutic agents such as doxorubicin with herbal products or other compounds that can enhance cytotoxicity without side effects is required. Thus, we aimed to observe the cytotoxicity of doxorubicin and sesoot (Garcinia picrorrhiza) fruit ethanolic extract (GpKar) on human fibroblast cells, BJ. This study used a post-test-only control randomized group design with n=3 and a number group of 5. The method used in this research is cell number, and viability was measured with (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Treatments consisted of a combination of doxorubicin (0.02 μg/ml) and GpKar of 66.47 µg/ml (DES1), 132.94 µg/ml (DES2) and 265.89 μg/ml (DES3). The data were analyzed using a one-way ANOVA and Duncan post hoc tests. DES3 showed the lowest viability among treatments (89.32%). DES1 and DES2 showed high viability (>90%), 97.93%, and 95.08%, respectively. Thus, the combination of doxorubicin (0.02 μg/ml) and GpKar (66.47 µg/ml) was considered safe for further use in the following assay. In summary, the combination of doxorubicin and GpKar showed high viability in normal fibroblast cells.https://ejournal.unisba.ac.id/index.php/gmhc/article/view/10985cytotoxicdoxorubicingarcinia picrorrhizahuman fibroblastmts assay |
| spellingShingle | Sri Utami Susi Endrini Lilian Batubara Nunung Ainur Rahmah Irfan Syarif Said Nafik Betharie Cendera Arrahmani Agung Novianto Hanna Sari Widya Kusuma Wahyu Widowati Cytotoxicity of Combination Doxorubicin and <i>Garcinia picrorrhiza</i> Fruit Extract on Fibroblast Cell Global Medical & Health Communication cytotoxic doxorubicin garcinia picrorrhiza human fibroblast mts assay |
| title | Cytotoxicity of Combination Doxorubicin and <i>Garcinia picrorrhiza</i> Fruit Extract on Fibroblast Cell |
| title_full | Cytotoxicity of Combination Doxorubicin and <i>Garcinia picrorrhiza</i> Fruit Extract on Fibroblast Cell |
| title_fullStr | Cytotoxicity of Combination Doxorubicin and <i>Garcinia picrorrhiza</i> Fruit Extract on Fibroblast Cell |
| title_full_unstemmed | Cytotoxicity of Combination Doxorubicin and <i>Garcinia picrorrhiza</i> Fruit Extract on Fibroblast Cell |
| title_short | Cytotoxicity of Combination Doxorubicin and <i>Garcinia picrorrhiza</i> Fruit Extract on Fibroblast Cell |
| title_sort | cytotoxicity of combination doxorubicin and i garcinia picrorrhiza i fruit extract on fibroblast cell |
| topic | cytotoxic doxorubicin garcinia picrorrhiza human fibroblast mts assay |
| url | https://ejournal.unisba.ac.id/index.php/gmhc/article/view/10985 |
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