Fast and reliable identification of axons, axon initial segments and dendrites with local field potential recording
The axon initial segment (AIS) is an essential neuronal compartment. It is usually where action potentials are initiated. Recent studies demonstrated that the AIS is a plastic structure that can be regulated by neuronal activity and by the activation of metabotropic receptors. Studying the AIS in li...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-10-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00429/full |
| _version_ | 1818497844455145472 |
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| author | Anders Victor ePetersen Emil Østergård Johansen Jean-François ePerrier |
| author_facet | Anders Victor ePetersen Emil Østergård Johansen Jean-François ePerrier |
| author_sort | Anders Victor ePetersen |
| collection | DOAJ |
| description | The axon initial segment (AIS) is an essential neuronal compartment. It is usually where action potentials are initiated. Recent studies demonstrated that the AIS is a plastic structure that can be regulated by neuronal activity and by the activation of metabotropic receptors. Studying the AIS in live tissue can be difficult because its identification is not always reliable. Here we provide a new technique allowing a fast and reliable identification of the AIS in live brain slice preparations. By simultaneous recoding of extracellular local field potentials and whole-cell patch-clamp recording of neurons, we can detect sinks caused by inward currents flowing across the membrane. We determine the location of the AIS by comparing the timing of these events with the action potential. We demonstrate that this method allows the unequivocal identification of the AIS of different types of neurons from the brain. |
| first_indexed | 2024-12-10T18:50:28Z |
| format | Article |
| id | doaj.art-07eff035cf6444939fa09b42b1894794 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-10T18:50:28Z |
| publishDate | 2015-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-07eff035cf6444939fa09b42b18947942022-12-22T01:37:20ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022015-10-01910.3389/fncel.2015.00429166691Fast and reliable identification of axons, axon initial segments and dendrites with local field potential recordingAnders Victor ePetersen0Emil Østergård Johansen1Jean-François ePerrier2University of CopenhagenUniversity of CopenhagenUniversity of CopenhagenThe axon initial segment (AIS) is an essential neuronal compartment. It is usually where action potentials are initiated. Recent studies demonstrated that the AIS is a plastic structure that can be regulated by neuronal activity and by the activation of metabotropic receptors. Studying the AIS in live tissue can be difficult because its identification is not always reliable. Here we provide a new technique allowing a fast and reliable identification of the AIS in live brain slice preparations. By simultaneous recoding of extracellular local field potentials and whole-cell patch-clamp recording of neurons, we can detect sinks caused by inward currents flowing across the membrane. We determine the location of the AIS by comparing the timing of these events with the action potential. We demonstrate that this method allows the unequivocal identification of the AIS of different types of neurons from the brain.http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00429/fullplasticityDendriteAxonmodulationaxon initial segmentspike triggered averaging |
| spellingShingle | Anders Victor ePetersen Emil Østergård Johansen Jean-François ePerrier Fast and reliable identification of axons, axon initial segments and dendrites with local field potential recording Frontiers in Cellular Neuroscience plasticity Dendrite Axon modulation axon initial segment spike triggered averaging |
| title | Fast and reliable identification of axons, axon initial segments and dendrites with local field potential recording |
| title_full | Fast and reliable identification of axons, axon initial segments and dendrites with local field potential recording |
| title_fullStr | Fast and reliable identification of axons, axon initial segments and dendrites with local field potential recording |
| title_full_unstemmed | Fast and reliable identification of axons, axon initial segments and dendrites with local field potential recording |
| title_short | Fast and reliable identification of axons, axon initial segments and dendrites with local field potential recording |
| title_sort | fast and reliable identification of axons axon initial segments and dendrites with local field potential recording |
| topic | plasticity Dendrite Axon modulation axon initial segment spike triggered averaging |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00429/full |
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