Brown Algae <i>Dictyopteris divaricata</i> Attenuates Adipogenesis by Modulating Adipocyte Differentiation and Promoting Lipolysis through Heme Oxygenase-1 Activation in 3T3-L1 Cells
The present study aims to explore the probable anti-adipogenesis effect of <i>Dictyopteris divaricata</i> (<i>D. divaricata</i>) in 3T3-L1 preadipocytes by regulating heme oxygenase-1 (HO-1). The extract of <i>D. divaricata</i> retarded lipid accretion and decreas...
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| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/22/2/91 |
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| author | Lakshi A. Dayarathne Seok-Chun Ko Mi-Jin Yim Jeong Min Lee Ji-Yul Kim Gun-Woo Oh Chul Hwan Kim Kyung Woo Kim Dae-Sung Lee Jae-Young Je |
| author_facet | Lakshi A. Dayarathne Seok-Chun Ko Mi-Jin Yim Jeong Min Lee Ji-Yul Kim Gun-Woo Oh Chul Hwan Kim Kyung Woo Kim Dae-Sung Lee Jae-Young Je |
| author_sort | Lakshi A. Dayarathne |
| collection | DOAJ |
| description | The present study aims to explore the probable anti-adipogenesis effect of <i>Dictyopteris divaricata</i> (<i>D. divaricata</i>) in 3T3-L1 preadipocytes by regulating heme oxygenase-1 (HO-1). The extract of <i>D. divaricata</i> retarded lipid accretion and decreased triglyceride (TG) content in 3T3-L1 adipocytes but increased free glycerol levels. Treatment with the extract inhibited lipogenesis by inhibiting protein expressions of fatty acid synthase (FAS) and lipoprotein lipase (LPL), whereas lipolysis increased by activating phosphorylation of hormone-sensitive lipase (p-HSL) and AMP-activated protein kinase (p-AMPK). The extract inhibited adipocyte differentiation of 3T3-L1 preadipocytes through down-regulating adipogenic transcription factors, including peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), and sterol regulatory element-binding protein 1 (SREBP1). This is attributed to the triggering of Wnt/β-catenin signaling. In addition, this study found that treatment with the extract activated HO-1 expression. Pharmacological approaches revealed that treatment with Zinc Protoporphyrin (ZnPP), an HO-1 inhibitor, resulted in an increase in lipid accumulation and a decrease in free glycerol levels. Finally, three adipogenic transcription factors, such as PPARγ, C/EBPα, and SREBP1, restored their expression in the presence of ZnPP. Analysis of chemical constituents revealed that the extract of <i>D. divaricata</i> is rich in 1,4-benzenediol, 7-tetradecenal, fucosterol, and n-hexadecanoic acid, which are known to have multiple pharmacological properties. |
| first_indexed | 2024-03-07T22:23:25Z |
| format | Article |
| id | doaj.art-07f0dd00053144ac9f6aa1d182901fa8 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-07T22:23:25Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-07f0dd00053144ac9f6aa1d182901fa82024-02-23T15:25:18ZengMDPI AGMarine Drugs1660-33972024-02-012229110.3390/md22020091Brown Algae <i>Dictyopteris divaricata</i> Attenuates Adipogenesis by Modulating Adipocyte Differentiation and Promoting Lipolysis through Heme Oxygenase-1 Activation in 3T3-L1 CellsLakshi A. Dayarathne0Seok-Chun Ko1Mi-Jin Yim2Jeong Min Lee3Ji-Yul Kim4Gun-Woo Oh5Chul Hwan Kim6Kyung Woo Kim7Dae-Sung Lee8Jae-Young Je9Department of Food and Nutrition, Pukyong National University, Busan 48513, Republic of KoreaNational Marine Biodiversity of Korea (MABIK), Seochun 33662, Republic of KoreaNational Marine Biodiversity of Korea (MABIK), Seochun 33662, Republic of KoreaNational Marine Biodiversity of Korea (MABIK), Seochun 33662, Republic of KoreaNational Marine Biodiversity of Korea (MABIK), Seochun 33662, Republic of KoreaNational Marine Biodiversity of Korea (MABIK), Seochun 33662, Republic of KoreaNational Marine Biodiversity of Korea (MABIK), Seochun 33662, Republic of KoreaNational Marine Biodiversity of Korea (MABIK), Seochun 33662, Republic of KoreaNational Marine Biodiversity of Korea (MABIK), Seochun 33662, Republic of KoreaMajor of Human Bioconvergence, Division of Smart Healthcare, Pukyong National University, Busan 48513, Republic of KoreaThe present study aims to explore the probable anti-adipogenesis effect of <i>Dictyopteris divaricata</i> (<i>D. divaricata</i>) in 3T3-L1 preadipocytes by regulating heme oxygenase-1 (HO-1). The extract of <i>D. divaricata</i> retarded lipid accretion and decreased triglyceride (TG) content in 3T3-L1 adipocytes but increased free glycerol levels. Treatment with the extract inhibited lipogenesis by inhibiting protein expressions of fatty acid synthase (FAS) and lipoprotein lipase (LPL), whereas lipolysis increased by activating phosphorylation of hormone-sensitive lipase (p-HSL) and AMP-activated protein kinase (p-AMPK). The extract inhibited adipocyte differentiation of 3T3-L1 preadipocytes through down-regulating adipogenic transcription factors, including peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), and sterol regulatory element-binding protein 1 (SREBP1). This is attributed to the triggering of Wnt/β-catenin signaling. In addition, this study found that treatment with the extract activated HO-1 expression. Pharmacological approaches revealed that treatment with Zinc Protoporphyrin (ZnPP), an HO-1 inhibitor, resulted in an increase in lipid accumulation and a decrease in free glycerol levels. Finally, three adipogenic transcription factors, such as PPARγ, C/EBPα, and SREBP1, restored their expression in the presence of ZnPP. Analysis of chemical constituents revealed that the extract of <i>D. divaricata</i> is rich in 1,4-benzenediol, 7-tetradecenal, fucosterol, and n-hexadecanoic acid, which are known to have multiple pharmacological properties.https://www.mdpi.com/1660-3397/22/2/91<i>Dictyopteris divaricata</i>adipogenesislipid accumulationlipolysisHO-1 |
| spellingShingle | Lakshi A. Dayarathne Seok-Chun Ko Mi-Jin Yim Jeong Min Lee Ji-Yul Kim Gun-Woo Oh Chul Hwan Kim Kyung Woo Kim Dae-Sung Lee Jae-Young Je Brown Algae <i>Dictyopteris divaricata</i> Attenuates Adipogenesis by Modulating Adipocyte Differentiation and Promoting Lipolysis through Heme Oxygenase-1 Activation in 3T3-L1 Cells Marine Drugs <i>Dictyopteris divaricata</i> adipogenesis lipid accumulation lipolysis HO-1 |
| title | Brown Algae <i>Dictyopteris divaricata</i> Attenuates Adipogenesis by Modulating Adipocyte Differentiation and Promoting Lipolysis through Heme Oxygenase-1 Activation in 3T3-L1 Cells |
| title_full | Brown Algae <i>Dictyopteris divaricata</i> Attenuates Adipogenesis by Modulating Adipocyte Differentiation and Promoting Lipolysis through Heme Oxygenase-1 Activation in 3T3-L1 Cells |
| title_fullStr | Brown Algae <i>Dictyopteris divaricata</i> Attenuates Adipogenesis by Modulating Adipocyte Differentiation and Promoting Lipolysis through Heme Oxygenase-1 Activation in 3T3-L1 Cells |
| title_full_unstemmed | Brown Algae <i>Dictyopteris divaricata</i> Attenuates Adipogenesis by Modulating Adipocyte Differentiation and Promoting Lipolysis through Heme Oxygenase-1 Activation in 3T3-L1 Cells |
| title_short | Brown Algae <i>Dictyopteris divaricata</i> Attenuates Adipogenesis by Modulating Adipocyte Differentiation and Promoting Lipolysis through Heme Oxygenase-1 Activation in 3T3-L1 Cells |
| title_sort | brown algae i dictyopteris divaricata i attenuates adipogenesis by modulating adipocyte differentiation and promoting lipolysis through heme oxygenase 1 activation in 3t3 l1 cells |
| topic | <i>Dictyopteris divaricata</i> adipogenesis lipid accumulation lipolysis HO-1 |
| url | https://www.mdpi.com/1660-3397/22/2/91 |
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