miR-23b-3p regulates differentiation of osteoclasts by targeting PTEN via the PI3k/AKT pathway
Introduction Osteoblasts and osteoclasts are cells of osteoblastic origin, and are vital in homeostasis of the skeleton. miRs are important for functioning, survival and differentiation of osteoclasts. It has been reported previously that miR-23b-3p is involved in osteoporosis and in regulation of d...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Termedia Publishing House
2019-09-01
|
| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/miR-23b-3p-regulates-differentiation-of-osteoclasts-by-targeting-PTEN-via-the-PI3k,110286,0,2.html |
| _version_ | 1797435292304539648 |
|---|---|
| author | Jiwei Chai Liang Xu Niansheng Liu |
| author_facet | Jiwei Chai Liang Xu Niansheng Liu |
| author_sort | Jiwei Chai |
| collection | DOAJ |
| description | Introduction
Osteoblasts and osteoclasts are cells of osteoblastic origin, and are vital in homeostasis of the skeleton. miRs are important for functioning, survival and differentiation of osteoclasts. It has been reported previously that miR-23b-3p is involved in osteoporosis and in regulation of differentiation of osteoblasts. It is also involved in the process of bone formation. However, the role of miR-23b-3p in differentiation of osteoclasts remains unexplored.
Material and methods
CSF-1 and ODF induced osteoclasts were used for the study. RNA isolation was done from TIB-71 cells. TRAP staining was done for TRAP-positive osteoclast formation. PIT assay for bone resorption was performed. For in vivo studies osteoclast-specific miR-23b-3p transgenic mice were developed.
Results
The levels of miR-23b-3p were upregulated in bone marrow monocytes during osteoclastogenesis with colony stimulating factor-1 and osteoclast differentiation factor induction, which suggests that miR-23b-3p plays a crucial role in differentiation of osteoclasts. Over-expression of miR-23b-3p in bone marrow monocytes leads to osteoclastogenesis, whereas the inhibition ameliorates it. We further studied the function of miR-23b-3p via PI3K/AKT targeting the PTEN pathway. In vivo, osteoclast-specific miR-23b-3p transgenic mice showed suppressed PTEN and elevated osteoclast activity, and the mice showed decreased bone mineral density.
Conclusions
The results suggest that miR-23b-3p regulates the differentiation of osteoclasts by targeting PTEN through the PI3K/AKT cascade. |
| first_indexed | 2024-03-09T10:46:03Z |
| format | Article |
| id | doaj.art-07f28cf964434d93bc9017ffb2cd3f55 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-03-09T10:46:03Z |
| publishDate | 2019-09-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-07f28cf964434d93bc9017ffb2cd3f552023-12-01T09:59:22ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512019-09-011861542155710.5114/aoms.2019.87520110286miR-23b-3p regulates differentiation of osteoclasts by targeting PTEN via the PI3k/AKT pathwayJiwei Chai0Liang Xu1Niansheng Liu2Second Ward of Trauma Surgery Department, Linyi People’s Hospital, Linyi, Shandong, ChinaDepartment of Surgery, Linyi Health School, Linyi, Shandong, ChinaThe First Ward of Trauma Surgery Department, Linyi People’s Hospital, Linyi, Shandong, ChinaIntroduction Osteoblasts and osteoclasts are cells of osteoblastic origin, and are vital in homeostasis of the skeleton. miRs are important for functioning, survival and differentiation of osteoclasts. It has been reported previously that miR-23b-3p is involved in osteoporosis and in regulation of differentiation of osteoblasts. It is also involved in the process of bone formation. However, the role of miR-23b-3p in differentiation of osteoclasts remains unexplored. Material and methods CSF-1 and ODF induced osteoclasts were used for the study. RNA isolation was done from TIB-71 cells. TRAP staining was done for TRAP-positive osteoclast formation. PIT assay for bone resorption was performed. For in vivo studies osteoclast-specific miR-23b-3p transgenic mice were developed. Results The levels of miR-23b-3p were upregulated in bone marrow monocytes during osteoclastogenesis with colony stimulating factor-1 and osteoclast differentiation factor induction, which suggests that miR-23b-3p plays a crucial role in differentiation of osteoclasts. Over-expression of miR-23b-3p in bone marrow monocytes leads to osteoclastogenesis, whereas the inhibition ameliorates it. We further studied the function of miR-23b-3p via PI3K/AKT targeting the PTEN pathway. In vivo, osteoclast-specific miR-23b-3p transgenic mice showed suppressed PTEN and elevated osteoclast activity, and the mice showed decreased bone mineral density. Conclusions The results suggest that miR-23b-3p regulates the differentiation of osteoclasts by targeting PTEN through the PI3K/AKT cascade.https://www.archivesofmedicalscience.com/miR-23b-3p-regulates-differentiation-of-osteoclasts-by-targeting-PTEN-via-the-PI3k,110286,0,2.htmlmir-23b-3posteoblastsosteoclastscsf-1odfptenpi3k/akt |
| spellingShingle | Jiwei Chai Liang Xu Niansheng Liu miR-23b-3p regulates differentiation of osteoclasts by targeting PTEN via the PI3k/AKT pathway Archives of Medical Science mir-23b-3p osteoblasts osteoclasts csf-1 odf pten pi3k/akt |
| title | miR-23b-3p regulates differentiation of osteoclasts by targeting PTEN via the PI3k/AKT pathway |
| title_full | miR-23b-3p regulates differentiation of osteoclasts by targeting PTEN via the PI3k/AKT pathway |
| title_fullStr | miR-23b-3p regulates differentiation of osteoclasts by targeting PTEN via the PI3k/AKT pathway |
| title_full_unstemmed | miR-23b-3p regulates differentiation of osteoclasts by targeting PTEN via the PI3k/AKT pathway |
| title_short | miR-23b-3p regulates differentiation of osteoclasts by targeting PTEN via the PI3k/AKT pathway |
| title_sort | mir 23b 3p regulates differentiation of osteoclasts by targeting pten via the pi3k akt pathway |
| topic | mir-23b-3p osteoblasts osteoclasts csf-1 odf pten pi3k/akt |
| url | https://www.archivesofmedicalscience.com/miR-23b-3p-regulates-differentiation-of-osteoclasts-by-targeting-PTEN-via-the-PI3k,110286,0,2.html |
| work_keys_str_mv | AT jiweichai mir23b3pregulatesdifferentiationofosteoclastsbytargetingptenviathepi3kaktpathway AT liangxu mir23b3pregulatesdifferentiationofosteoclastsbytargetingptenviathepi3kaktpathway AT nianshengliu mir23b3pregulatesdifferentiationofosteoclastsbytargetingptenviathepi3kaktpathway |