The Effect of Resistance Training on PI3K/mTORc1 Signaling in Left Ventricle of Diabetic Rats
Background: Clinical evidence supports the influential role of genetic factors and intracellular signaling pathways in physiological cardiac hypertrophy. This study aimed to assess the response of the PI3K/mTORc1 signaling pathway in cardiac tissue to resistance training in obese rats with Type 2 Di...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences and Health Services
2021-08-01
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| Series: | Research in Molecular Medicine |
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| Online Access: | http://rmm.mazums.ac.ir/article-1-435-en.html |
| _version_ | 1797973894505693184 |
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| author | Mohammad Dastyar Mojtaba Eizadi Mania Roozbayani |
| author_facet | Mohammad Dastyar Mojtaba Eizadi Mania Roozbayani |
| author_sort | Mohammad Dastyar |
| collection | DOAJ |
| description | Background: Clinical evidence supports the influential role of genetic factors and intracellular signaling pathways in physiological cardiac hypertrophy. This study aimed to assess the response of the PI3K/mTORc1 signaling pathway in cardiac tissue to resistance training in obese rats with Type 2 Diabetes (T2D).
Materials and Methods: A total of 21 male Wistar rats (Mean±SD weight: 220±20 g) were obese by 6 weeks High-Fat Diet (HFD) and randomly assigned to 1) non-diabetic, 2) control T2D, and 3) exercise diabetic groups. T2D was induced by intraperitoneal injection of streptozotocin (30 mg/kg) for diabetic groups. The exercise group did the resistance exercise program (5 times per week for 6 weeks). After exercise training, PI3K and mTORc1 expression in the left ventricle and the ratio of the left ventricle to heart and heart to body weight were compared between groups. The obtained data were compared by 1-way Analysis of Variance (ANOVA) (P<0.05).
Results: Induction of diabetes resulted in significant decrease in all mentioned variables in control diabetic to non-diabetic rats (PI3K; P=0.021, mTORc1; P=0.004, left ventricle/heart weight; P=0.045, heart/body weight; P=0.035). Significant increase was observed in all variables (PI3K; P=0.028, mTORc1; P=0.015, left ventricular/heart weight; P=0.002, heart/body weight; P=0.001) in response to resistance training compared to the control rat.
Conclusion: Based on our results, cardiac hypertrophy in studied diabetic rats can be attributed to improved PI3K/mTORc1 signaling in response to resistance training. Exploring the exact mechanisms responsible for these changes in response to exercise requires further molecular-cellular studies. |
| first_indexed | 2024-04-11T04:11:26Z |
| format | Article |
| id | doaj.art-07f39ca7ef38432cb1e4005b91a660fc |
| institution | Directory Open Access Journal |
| issn | 2322-1348 2322-133X |
| language | English |
| last_indexed | 2024-04-11T04:11:26Z |
| publishDate | 2021-08-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-07f39ca7ef38432cb1e4005b91a660fc2023-01-01T05:41:47ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2021-08-0193173180The Effect of Resistance Training on PI3K/mTORc1 Signaling in Left Ventricle of Diabetic RatsMohammad Dastyar0Mojtaba Eizadi1Mania Roozbayani2 Department of Exercise physiology, Borujerd Branch, Islamic Azad University, Borujerd, Iran Department of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran Department of Exercise physiology, Borujerd Branch, Islamic Azad University, Borujerd, Iran Background: Clinical evidence supports the influential role of genetic factors and intracellular signaling pathways in physiological cardiac hypertrophy. This study aimed to assess the response of the PI3K/mTORc1 signaling pathway in cardiac tissue to resistance training in obese rats with Type 2 Diabetes (T2D). Materials and Methods: A total of 21 male Wistar rats (Mean±SD weight: 220±20 g) were obese by 6 weeks High-Fat Diet (HFD) and randomly assigned to 1) non-diabetic, 2) control T2D, and 3) exercise diabetic groups. T2D was induced by intraperitoneal injection of streptozotocin (30 mg/kg) for diabetic groups. The exercise group did the resistance exercise program (5 times per week for 6 weeks). After exercise training, PI3K and mTORc1 expression in the left ventricle and the ratio of the left ventricle to heart and heart to body weight were compared between groups. The obtained data were compared by 1-way Analysis of Variance (ANOVA) (P<0.05). Results: Induction of diabetes resulted in significant decrease in all mentioned variables in control diabetic to non-diabetic rats (PI3K; P=0.021, mTORc1; P=0.004, left ventricle/heart weight; P=0.045, heart/body weight; P=0.035). Significant increase was observed in all variables (PI3K; P=0.028, mTORc1; P=0.015, left ventricular/heart weight; P=0.002, heart/body weight; P=0.001) in response to resistance training compared to the control rat. Conclusion: Based on our results, cardiac hypertrophy in studied diabetic rats can be attributed to improved PI3K/mTORc1 signaling in response to resistance training. Exploring the exact mechanisms responsible for these changes in response to exercise requires further molecular-cellular studies.http://rmm.mazums.ac.ir/article-1-435-en.htmlheart hypertrophyexercisegene’s expressiondiabetes |
| spellingShingle | Mohammad Dastyar Mojtaba Eizadi Mania Roozbayani The Effect of Resistance Training on PI3K/mTORc1 Signaling in Left Ventricle of Diabetic Rats Research in Molecular Medicine heart hypertrophy exercise gene’s expression diabetes |
| title | The Effect of Resistance Training on PI3K/mTORc1 Signaling in Left Ventricle of Diabetic Rats |
| title_full | The Effect of Resistance Training on PI3K/mTORc1 Signaling in Left Ventricle of Diabetic Rats |
| title_fullStr | The Effect of Resistance Training on PI3K/mTORc1 Signaling in Left Ventricle of Diabetic Rats |
| title_full_unstemmed | The Effect of Resistance Training on PI3K/mTORc1 Signaling in Left Ventricle of Diabetic Rats |
| title_short | The Effect of Resistance Training on PI3K/mTORc1 Signaling in Left Ventricle of Diabetic Rats |
| title_sort | effect of resistance training on pi3k mtorc1 signaling in left ventricle of diabetic rats |
| topic | heart hypertrophy exercise gene’s expression diabetes |
| url | http://rmm.mazums.ac.ir/article-1-435-en.html |
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