Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken
Cefepime is a broad-spectrum semi synthetic β-lactamase resistant fourth generation cephalosporin. Looking to potential for clinical use, pharmacokinetics of cefepime following single intravenous and intramuscular (IM) dose (100mg/kg b. wt.) in healthy and experimentally Salmonella typhimurium infe...
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| Format: | Article |
| Language: | English |
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South Valley University
2018-07-01
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| Series: | SVU-International Journal of Veterinary Sciences |
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| Online Access: | https://portal.svu.edu.eg/svu-src/index.php/ijvs/article/view/10 |
| _version_ | 1818751101681270784 |
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| author | Taha A. Attia |
| author_facet | Taha A. Attia |
| author_sort | Taha A. Attia |
| collection | DOAJ |
| description | Cefepime is a broad-spectrum semi synthetic β-lactamase resistant fourth generation cephalosporin. Looking to potential for clinical use, pharmacokinetics of cefepime following single intravenous and intramuscular (IM) dose (100mg/kg b. wt.) in healthy and experimentally Salmonella typhimurium infected broiler chickens were determined. Cefepime concentration in plasma samples was determined by reverse-phase high performance liquid chromatography with mobile phase. The mobile phase was a mixture of 10mM phosphate buffer (pH 7): Methanol; 75:25 was always freshly prepared. Flow rates were 1 ml/min. UV detection was performed at 256 nm, injection volume was 20 µl. After a single intravenous injection, cefepime reached its maximum serum concentrations of 4.28 ± 0.37μg/ml in normal chickens, while in the infected chickens, the maximum serum concentration was 2.62 ± 0.72 μg/ml. Cefepime was eliminated after intravenous injection with half-life (t1/2 β) of 4.608 ± 0.145 h in normal which significantly longer than 4.19 ± 0.158 h in infected chickens. The mean residence time (MRT) was 6.51 ± 0.189h in normal vs 5.86±0.18 h in infected chickens. After IM administration the drug reached its maximum serum concentrations of 193.06 ± 2.27μg/ml at maximum time of 1.138 ± 0.012 h in normal, while in infected chickens the maximum serum concentrations was 132.93 ± 1.53μg/ml attained at maximum time of 1.265 ± 0.013 h. In conclusion a cefepime at dose of 100 mg/kg administered intravenously or IM at 24 h intervals may provide successful treatment of chicken infected with Salmonella typhimurium. |
| first_indexed | 2024-12-18T04:30:13Z |
| format | Article |
| id | doaj.art-07f8918873744e4285049b42f1ced7f3 |
| institution | Directory Open Access Journal |
| issn | 2535-1826 2535-1826 |
| language | English |
| last_indexed | 2024-12-18T04:30:13Z |
| publishDate | 2018-07-01 |
| publisher | South Valley University |
| record_format | Article |
| series | SVU-International Journal of Veterinary Sciences |
| spelling | doaj.art-07f8918873744e4285049b42f1ced7f32022-12-21T21:20:59ZengSouth Valley UniversitySVU-International Journal of Veterinary Sciences2535-18262535-18262018-07-01116681Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler ChickenTaha A. Attia0Faculty of Veterinary Medicine, University of Sadat CityCefepime is a broad-spectrum semi synthetic β-lactamase resistant fourth generation cephalosporin. Looking to potential for clinical use, pharmacokinetics of cefepime following single intravenous and intramuscular (IM) dose (100mg/kg b. wt.) in healthy and experimentally Salmonella typhimurium infected broiler chickens were determined. Cefepime concentration in plasma samples was determined by reverse-phase high performance liquid chromatography with mobile phase. The mobile phase was a mixture of 10mM phosphate buffer (pH 7): Methanol; 75:25 was always freshly prepared. Flow rates were 1 ml/min. UV detection was performed at 256 nm, injection volume was 20 µl. After a single intravenous injection, cefepime reached its maximum serum concentrations of 4.28 ± 0.37μg/ml in normal chickens, while in the infected chickens, the maximum serum concentration was 2.62 ± 0.72 μg/ml. Cefepime was eliminated after intravenous injection with half-life (t1/2 β) of 4.608 ± 0.145 h in normal which significantly longer than 4.19 ± 0.158 h in infected chickens. The mean residence time (MRT) was 6.51 ± 0.189h in normal vs 5.86±0.18 h in infected chickens. After IM administration the drug reached its maximum serum concentrations of 193.06 ± 2.27μg/ml at maximum time of 1.138 ± 0.012 h in normal, while in infected chickens the maximum serum concentrations was 132.93 ± 1.53μg/ml attained at maximum time of 1.265 ± 0.013 h. In conclusion a cefepime at dose of 100 mg/kg administered intravenously or IM at 24 h intervals may provide successful treatment of chicken infected with Salmonella typhimurium.https://portal.svu.edu.eg/svu-src/index.php/ijvs/article/view/10CefepimeSalmonella typhimuriumhigh performance liquid chromatographybroiler chicken |
| spellingShingle | Taha A. Attia Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken SVU-International Journal of Veterinary Sciences Cefepime Salmonella typhimurium high performance liquid chromatography broiler chicken |
| title | Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
| title_full | Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
| title_fullStr | Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
| title_full_unstemmed | Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
| title_short | Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
| title_sort | disposition kinetics of cefepime in healthy and experimental salmonella typhimurium infected broiler chicken |
| topic | Cefepime Salmonella typhimurium high performance liquid chromatography broiler chicken |
| url | https://portal.svu.edu.eg/svu-src/index.php/ijvs/article/view/10 |
| work_keys_str_mv | AT tahaaattia dispositionkineticsofcefepimeinhealthyandexperimentalsalmonellatyphimuriuminfectedbroilerchicken |