Unlabeled lysophosphatidic acid receptor binding in free solution as determined by a compensated interferometric reader
Native interactions between lysophospholipids (LPs) and their cognate LP receptors are difficult to measure because of lipophilicity and/or the adhesive properties of lipids, which contribute to high levels of nonspecific binding in cell membrane preparations. Here, we report development of a free-s...
Main Authors: | Manisha Ray, Kazufumi Nagai, Yasuyuki Kihara, Amanda Kussrow, Michael N. Kammer, Aaron Frantz, Darryl J. Bornhop, Jerold Chun |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-08-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520434923 |
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