Development and Statistical Validation of E-Probe Diagnostic Nucleic Acid Analysis (EDNA) Assays for the Detection of Citrus Pathogens from Raw High-Throughput Sequencing Data

Development and Statistical Validation of E-Probe Diagnostic Nucleic Acid Analysis (EDNA) Assays for the Detection of Citrus Pathogens from Raw High-Throughput Sequencing Data

The cost for high-throughput sequencing (HTS) has decreased significantly and has made it possible for the application of this technology for routine plant diagnostics. There are constraints to the use of HTS as a diagnostic tool, including the need for dedicated personnel with a bioinformatic backg...

Full description

Bibliographic Details
Main Authors: Tyler Dang, Huizi Wang, Andres S. Espindola, Josh Habiger, Georgios Vidalakis, Kitty Cardwell
Format: Article
Language:English
Published: The American Phytopathological Society 2023-06-01
Series:PhytoFrontiers
Subjects:
assay validation
high-throughput sequence (HTS) diagnostics
limit of detection
MiFi
next-generation sequencing (NGS)
Online Access:https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-05-22-0047-FI

Similar Items

Similar Items