Promotion of Growth of Alfalfa by <i>Erwinia persicina</i> Cp2 Exopolysaccharides under NaCl Stress
Soil salinity poses a significant challenge to plant growth and agricultural productivity. Research demonstrated the potential of exopolysaccharides (EPS) to enhance plant growth and improve resistance to abiotic stress. Nevertheless, the precise regulatory mechanism by which EPS mediates salt toler...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/8/2129 |
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| author | Haiyan Chen Zhenhai Jin Rong Huang Linxin He Wangjun Tian Liang Zhao Zhenfen Zhang |
| author_facet | Haiyan Chen Zhenhai Jin Rong Huang Linxin He Wangjun Tian Liang Zhao Zhenfen Zhang |
| author_sort | Haiyan Chen |
| collection | DOAJ |
| description | Soil salinity poses a significant challenge to plant growth and agricultural productivity. Research demonstrated the potential of exopolysaccharides (EPS) to enhance plant growth and improve resistance to abiotic stress. Nevertheless, the precise regulatory mechanism by which EPS mediates salt tolerance in alfalfa (<i>Medicago sativa</i>) remains largely unexplored. To investigate the protective effects of EPS from <i>Erwinia persicina</i> Cp2 in enhancing salt stress tolerance in alfalfa, a hydroponic experiment was conducted on the ‘Aohan’ cultivar of alfalfa, and changes in germination rate, biomass, chlorophyll content, electrolyte leakage (EL), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde (MDA), proline (Pro), soluble sugar (SS), soluble protein (SP), and activities of superoxide dismutase (SOD) and peroxidase (POD) were analyzed with and without Cp2 EPS under 75 mmol/L NaCl conditions. The results show that the exposure of alfalfa to salt conditions seriously inhibited its germination and growth. However, it is interesting that the application of Cp2 EPS greatly alleviated the damage of salt stress on alfalfa and promoted the germination of alfalfa as well as the root length, seedling length, fresh weight, and dry weight of the plants. In addition, the increases in MDA, H<sub>2</sub>O<sub>2</sub> content, and EL rate caused by salt stress were inhibited after Cp2 EPS treatment, while chlorophyll, Pro, SP, and SS contents as well as SOD and CAT activities were increased. Therefore, Cp2 EPS can significantly alleviate the toxic effect of 75 mmol/L NaCl stress on alfalfa, and comprehensive analysis shows that 1.5 g/L Cp2 EPS had the best alleviating effect on alfalfa at this salt stress concentration. This study lays a practical and theoretical foundation for the development of biogenic agents used to alleviate the growth of alfalfa in saline alkali soil. |
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| format | Article |
| id | doaj.art-080003b9662f45c59c3bebe07880b665 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T00:11:09Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-080003b9662f45c59c3bebe07880b6652023-11-18T23:55:24ZengMDPI AGAgronomy2073-43952023-08-01138212910.3390/agronomy13082129Promotion of Growth of Alfalfa by <i>Erwinia persicina</i> Cp2 Exopolysaccharides under NaCl StressHaiyan Chen0Zhenhai Jin1Rong Huang2Linxin He3Wangjun Tian4Liang Zhao5Zhenfen Zhang6Key Laboratory of Grassland Ecosystem, Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, ChinaKey Laboratory of Grassland Ecosystem, Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, ChinaKey Laboratory of Grassland Ecosystem, Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, ChinaKey Laboratory of Grassland Ecosystem, Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, ChinaKey Laboratory of Grassland Ecosystem, Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, ChinaKey Laboratory of Grassland Ecosystem, Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, ChinaKey Laboratory of Grassland Ecosystem, Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, ChinaSoil salinity poses a significant challenge to plant growth and agricultural productivity. Research demonstrated the potential of exopolysaccharides (EPS) to enhance plant growth and improve resistance to abiotic stress. Nevertheless, the precise regulatory mechanism by which EPS mediates salt tolerance in alfalfa (<i>Medicago sativa</i>) remains largely unexplored. To investigate the protective effects of EPS from <i>Erwinia persicina</i> Cp2 in enhancing salt stress tolerance in alfalfa, a hydroponic experiment was conducted on the ‘Aohan’ cultivar of alfalfa, and changes in germination rate, biomass, chlorophyll content, electrolyte leakage (EL), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde (MDA), proline (Pro), soluble sugar (SS), soluble protein (SP), and activities of superoxide dismutase (SOD) and peroxidase (POD) were analyzed with and without Cp2 EPS under 75 mmol/L NaCl conditions. The results show that the exposure of alfalfa to salt conditions seriously inhibited its germination and growth. However, it is interesting that the application of Cp2 EPS greatly alleviated the damage of salt stress on alfalfa and promoted the germination of alfalfa as well as the root length, seedling length, fresh weight, and dry weight of the plants. In addition, the increases in MDA, H<sub>2</sub>O<sub>2</sub> content, and EL rate caused by salt stress were inhibited after Cp2 EPS treatment, while chlorophyll, Pro, SP, and SS contents as well as SOD and CAT activities were increased. Therefore, Cp2 EPS can significantly alleviate the toxic effect of 75 mmol/L NaCl stress on alfalfa, and comprehensive analysis shows that 1.5 g/L Cp2 EPS had the best alleviating effect on alfalfa at this salt stress concentration. This study lays a practical and theoretical foundation for the development of biogenic agents used to alleviate the growth of alfalfa in saline alkali soil.https://www.mdpi.com/2073-4395/13/8/2129alfalfasalt stress<i>Erwinia persicina</i> Cp2 EPSseed germinationseedling growth |
| spellingShingle | Haiyan Chen Zhenhai Jin Rong Huang Linxin He Wangjun Tian Liang Zhao Zhenfen Zhang Promotion of Growth of Alfalfa by <i>Erwinia persicina</i> Cp2 Exopolysaccharides under NaCl Stress Agronomy alfalfa salt stress <i>Erwinia persicina</i> Cp2 EPS seed germination seedling growth |
| title | Promotion of Growth of Alfalfa by <i>Erwinia persicina</i> Cp2 Exopolysaccharides under NaCl Stress |
| title_full | Promotion of Growth of Alfalfa by <i>Erwinia persicina</i> Cp2 Exopolysaccharides under NaCl Stress |
| title_fullStr | Promotion of Growth of Alfalfa by <i>Erwinia persicina</i> Cp2 Exopolysaccharides under NaCl Stress |
| title_full_unstemmed | Promotion of Growth of Alfalfa by <i>Erwinia persicina</i> Cp2 Exopolysaccharides under NaCl Stress |
| title_short | Promotion of Growth of Alfalfa by <i>Erwinia persicina</i> Cp2 Exopolysaccharides under NaCl Stress |
| title_sort | promotion of growth of alfalfa by i erwinia persicina i cp2 exopolysaccharides under nacl stress |
| topic | alfalfa salt stress <i>Erwinia persicina</i> Cp2 EPS seed germination seedling growth |
| url | https://www.mdpi.com/2073-4395/13/8/2129 |
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