HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)

Micromorphological and anatomical investigations of trichomes on vegetative and generative organs of the drug eyebright (Euphrasia stricta D. Wolff ex J.F. Lehm.) were carried out. Additionally, identification of the main components of the secreted products was conducted. The following types of gla...

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Main Authors: Weronika Haratym, Elżbieta Weryszko-Chmielewska
Format: Article
Language:English
Published: University of Life Sciences in Lublin - Publishing House 2017-10-01
Series:Acta Scientiarum Polonorum: Hortorum Cultus
Subjects:
Online Access:https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2374
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author Weronika Haratym
Elżbieta Weryszko-Chmielewska
author_facet Weronika Haratym
Elżbieta Weryszko-Chmielewska
author_sort Weronika Haratym
collection DOAJ
description Micromorphological and anatomical investigations of trichomes on vegetative and generative organs of the drug eyebright (Euphrasia stricta D. Wolff ex J.F. Lehm.) were carried out. Additionally, identification of the main components of the secreted products was conducted. The following types of glandular structures were found: capitate trichomes (i) with a unicellular head, a neck cell, and a 2-celled stalk, (ii) with a unicellular head, a neck cell, and 3-celled stalk, (iii) with a bicellular head and sporadically with a 3- or 4-celled head; moreover, on the surface of corolla unicellular and 2–3-celled clavate trichomes, ribbon-like trichomes, and papillae were present. Using fluorescence microscopy and histochemical tests, various substances such as lipids, phenolic compounds, terpenes containing steroids, terpenoids, sesquiterpenes, tannins, and flavonoids were determined but only in the capitate trichomes. The analysis of the essential oil showed that its content in the dry herb of E. stricta was on average 0.257%. Gas chromatography revealed the presence of 28 compounds.
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spelling doaj.art-08025b249fad44799c6d77fa76c2064d2023-12-12T08:01:17ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052017-10-01165HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)Weronika HaratymElżbieta Weryszko-Chmielewska Micromorphological and anatomical investigations of trichomes on vegetative and generative organs of the drug eyebright (Euphrasia stricta D. Wolff ex J.F. Lehm.) were carried out. Additionally, identification of the main components of the secreted products was conducted. The following types of glandular structures were found: capitate trichomes (i) with a unicellular head, a neck cell, and a 2-celled stalk, (ii) with a unicellular head, a neck cell, and 3-celled stalk, (iii) with a bicellular head and sporadically with a 3- or 4-celled head; moreover, on the surface of corolla unicellular and 2–3-celled clavate trichomes, ribbon-like trichomes, and papillae were present. Using fluorescence microscopy and histochemical tests, various substances such as lipids, phenolic compounds, terpenes containing steroids, terpenoids, sesquiterpenes, tannins, and flavonoids were determined but only in the capitate trichomes. The analysis of the essential oil showed that its content in the dry herb of E. stricta was on average 0.257%. Gas chromatography revealed the presence of 28 compounds. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2374chemical compoundsdrug eyebrighthistochemistrymicromorphologysecretory structure
spellingShingle Weronika Haratym
Elżbieta Weryszko-Chmielewska
HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)
Acta Scientiarum Polonorum: Hortorum Cultus
chemical compounds
drug eyebright
histochemistry
micromorphology
secretory structure
title HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)
title_full HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)
title_fullStr HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)
title_full_unstemmed HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)
title_short HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)
title_sort histochemical investigation of trichomes and chemical composition of essential oil from euphrasia stricta d wolff ex j f lehm orobanchaceae
topic chemical compounds
drug eyebright
histochemistry
micromorphology
secretory structure
url https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2374
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