Mapping and Functional Characterization of Stigma Exposed 1, a DUF1005 Gene Controlling Petal and Stigma Cells in Mungbean (Vigna radiata)
Flowers with exposed stigma increase the outcrossing rate and are useful in developing improved hybrid crop cultivars. This exposure results mainly from the cellular morphology of the petal and pistil, but what affects the formation of the petal and pistil in the late developmental stages is less un...
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2020.575922/full |
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| author | Yun Lin Kularb Laosatit Jingbin Chen Xingxing Yuan Ranran Wu Kitiya Amkul Xin Chen Prakit Somta Prakit Somta |
| author_facet | Yun Lin Kularb Laosatit Jingbin Chen Xingxing Yuan Ranran Wu Kitiya Amkul Xin Chen Prakit Somta Prakit Somta |
| author_sort | Yun Lin |
| collection | DOAJ |
| description | Flowers with exposed stigma increase the outcrossing rate and are useful in developing improved hybrid crop cultivars. This exposure results mainly from the cellular morphology of the petal and pistil, but what affects the formation of the petal and pistil in the late developmental stages is less understood. Here, we characterized a novel floral mutant in mungbean (Vigna radiata), stigma exposed 1 (se1), which displays irregular petals and pistils. Floral organ initiation in the se1 mutant was normal, but petal and pistil growth malfunctioned during late development. A histological analysis revealed that the se1 mutant had wrinkled petals with knotted structures and elongated styles. The cellular morphology of the epidermal layers of the se1 petals was deformed, while the cell lengths in the styles increased. A genetic analysis indicated that the se1 phenotype is controlled by a single recessive gene, and it was mapped to chromosome 11. A sequence analysis suggested that a DUF1005-encoding gene, LOC106777793, is the gene controlling the se1 phenotype. The se1 mutant possessed a single-nucleotide polymorphism that resulted in an amino acid change in VrDUF1005. Overexpression of VrDUF1005 in Arabidopsis resulted in rolling leaves and reduced floral size. Consequently, we proposed that VrSE1 functions to modulate cell division in petals and cell expansion in styles during the late developmental stages in mungbean. The se1 mutant is a new genetic resource for mung bean hybrid breeding. |
| first_indexed | 2024-12-11T17:20:38Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T17:20:38Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-0803139ab4394067b58a728958b4ddc42022-12-22T00:57:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-11-011110.3389/fpls.2020.575922575922Mapping and Functional Characterization of Stigma Exposed 1, a DUF1005 Gene Controlling Petal and Stigma Cells in Mungbean (Vigna radiata)Yun Lin0Kularb Laosatit1Jingbin Chen2Xingxing Yuan3Ranran Wu4Kitiya Amkul5Xin Chen6Prakit Somta7Prakit Somta8Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaDepartment of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, ThailandInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaDepartment of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, ThailandInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaDepartment of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, ThailandCenter of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, ThailandFlowers with exposed stigma increase the outcrossing rate and are useful in developing improved hybrid crop cultivars. This exposure results mainly from the cellular morphology of the petal and pistil, but what affects the formation of the petal and pistil in the late developmental stages is less understood. Here, we characterized a novel floral mutant in mungbean (Vigna radiata), stigma exposed 1 (se1), which displays irregular petals and pistils. Floral organ initiation in the se1 mutant was normal, but petal and pistil growth malfunctioned during late development. A histological analysis revealed that the se1 mutant had wrinkled petals with knotted structures and elongated styles. The cellular morphology of the epidermal layers of the se1 petals was deformed, while the cell lengths in the styles increased. A genetic analysis indicated that the se1 phenotype is controlled by a single recessive gene, and it was mapped to chromosome 11. A sequence analysis suggested that a DUF1005-encoding gene, LOC106777793, is the gene controlling the se1 phenotype. The se1 mutant possessed a single-nucleotide polymorphism that resulted in an amino acid change in VrDUF1005. Overexpression of VrDUF1005 in Arabidopsis resulted in rolling leaves and reduced floral size. Consequently, we proposed that VrSE1 functions to modulate cell division in petals and cell expansion in styles during the late developmental stages in mungbean. The se1 mutant is a new genetic resource for mung bean hybrid breeding.https://www.frontiersin.org/articles/10.3389/fpls.2020.575922/fullexposed stigmafloral structurecell morphologygene cloningmungbean |
| spellingShingle | Yun Lin Kularb Laosatit Jingbin Chen Xingxing Yuan Ranran Wu Kitiya Amkul Xin Chen Prakit Somta Prakit Somta Mapping and Functional Characterization of Stigma Exposed 1, a DUF1005 Gene Controlling Petal and Stigma Cells in Mungbean (Vigna radiata) Frontiers in Plant Science exposed stigma floral structure cell morphology gene cloning mungbean |
| title | Mapping and Functional Characterization of Stigma Exposed 1, a DUF1005 Gene Controlling Petal and Stigma Cells in Mungbean (Vigna radiata) |
| title_full | Mapping and Functional Characterization of Stigma Exposed 1, a DUF1005 Gene Controlling Petal and Stigma Cells in Mungbean (Vigna radiata) |
| title_fullStr | Mapping and Functional Characterization of Stigma Exposed 1, a DUF1005 Gene Controlling Petal and Stigma Cells in Mungbean (Vigna radiata) |
| title_full_unstemmed | Mapping and Functional Characterization of Stigma Exposed 1, a DUF1005 Gene Controlling Petal and Stigma Cells in Mungbean (Vigna radiata) |
| title_short | Mapping and Functional Characterization of Stigma Exposed 1, a DUF1005 Gene Controlling Petal and Stigma Cells in Mungbean (Vigna radiata) |
| title_sort | mapping and functional characterization of stigma exposed 1 a duf1005 gene controlling petal and stigma cells in mungbean vigna radiata |
| topic | exposed stigma floral structure cell morphology gene cloning mungbean |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2020.575922/full |
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