Phenotypic and Genotypic Antibiotic Resistance Patterns in Helicobacter pylori Strains From Ethnically Diverse Population in México
Helicobacter pylori strains carry a range of mutations in genes that confer antimicrobial resistance and restrict the available options to treat the infection. Latin America is a region that conserve a large number of indigenous communities relatively isolated that practice a traditional medicine wi...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2020.539115/full |
| _version_ | 1818570020822712320 |
|---|---|
| author | Margarita Camorlinga-Ponce Alejandro Gómez-Delgado Emmanuel Aguilar-Zamora Emmanuel Aguilar-Zamora Roberto C. Torres Silvia Giono-Cerezo Antonio Escobar-Ogaz Javier Torres |
| author_facet | Margarita Camorlinga-Ponce Alejandro Gómez-Delgado Emmanuel Aguilar-Zamora Emmanuel Aguilar-Zamora Roberto C. Torres Silvia Giono-Cerezo Antonio Escobar-Ogaz Javier Torres |
| author_sort | Margarita Camorlinga-Ponce |
| collection | DOAJ |
| description | Helicobacter pylori strains carry a range of mutations in genes that confer antimicrobial resistance and restrict the available options to treat the infection. Latin America is a region that conserve a large number of indigenous communities relatively isolated that practice a traditional medicine without consumption of drugs. We hypothesized that rates of antibiotic resistance are lower in these communities. Recent progress in whole-genome sequencing has allowed the study of drug susceptibility by searching for the known mutations associated with antibiotic resistance. The aim of this work was to study trends of antibiotic resistance over a 20-year period in Mexican H. pylori strains and to compare susceptibility between strains from Mexican mestizos and from indigenous population; we also aimed to learn the prevalence of mutational patterns in genes gyrA, gyrB, rdxA, frxA, rpsU, omp11, dppA, and 23S rRNA and its association with phenotypic tests. Resistance to clarithromycin, metronidazole, amoxicillin and levofloxacin was determined in167 H. pylori isolates by E-test, and the occurrence of mutational patterns in specific genes was determined by whole genome sequencing (WGS). The trend of resistance over 20 years in mestizo isolates showed significant resistant increase for clarithromycin and levofloxacin to frequencies that banned its clinical use. Resistance in H. pylori isolates of native communities was lower for all antibiotics tested. Phenotypic resistance showed good to moderate correlation with genotypic tests. Genetic methods for characterizing antibiotic resistance require further validation in each population. |
| first_indexed | 2024-12-14T06:55:19Z |
| format | Article |
| id | doaj.art-0804f78a257e412fa1ce16a27148818c |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-14T06:55:19Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-0804f78a257e412fa1ce16a27148818c2022-12-21T23:12:42ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-02-011010.3389/fcimb.2020.539115539115Phenotypic and Genotypic Antibiotic Resistance Patterns in Helicobacter pylori Strains From Ethnically Diverse Population in MéxicoMargarita Camorlinga-Ponce0Alejandro Gómez-Delgado1Emmanuel Aguilar-Zamora2Emmanuel Aguilar-Zamora3Roberto C. Torres4Silvia Giono-Cerezo5Antonio Escobar-Ogaz6Javier Torres7Unidad de Investigación en Enfermedades Infecciosas, UMAE Pediatría, Instituto Mexicano del Seguro Social, Ciudad de México, MexicoUnidad de Investigación en Enfermedades Infecciosas, UMAE Pediatría, Instituto Mexicano del Seguro Social, Ciudad de México, MexicoUnidad de Investigación en Enfermedades Infecciosas, UMAE Pediatría, Instituto Mexicano del Seguro Social, Ciudad de México, MexicoPosgrado en Biomedicina y Biotecnología Molecular, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MexicoUnidad de Investigación en Enfermedades Infecciosas, UMAE Pediatría, Instituto Mexicano del Seguro Social, Ciudad de México, MexicoDepartamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, MexicoUnidad de Investigación en Enfermedades Infecciosas, UMAE Pediatría, Instituto Mexicano del Seguro Social, Ciudad de México, MexicoUnidad de Investigación en Enfermedades Infecciosas, UMAE Pediatría, Instituto Mexicano del Seguro Social, Ciudad de México, MexicoHelicobacter pylori strains carry a range of mutations in genes that confer antimicrobial resistance and restrict the available options to treat the infection. Latin America is a region that conserve a large number of indigenous communities relatively isolated that practice a traditional medicine without consumption of drugs. We hypothesized that rates of antibiotic resistance are lower in these communities. Recent progress in whole-genome sequencing has allowed the study of drug susceptibility by searching for the known mutations associated with antibiotic resistance. The aim of this work was to study trends of antibiotic resistance over a 20-year period in Mexican H. pylori strains and to compare susceptibility between strains from Mexican mestizos and from indigenous population; we also aimed to learn the prevalence of mutational patterns in genes gyrA, gyrB, rdxA, frxA, rpsU, omp11, dppA, and 23S rRNA and its association with phenotypic tests. Resistance to clarithromycin, metronidazole, amoxicillin and levofloxacin was determined in167 H. pylori isolates by E-test, and the occurrence of mutational patterns in specific genes was determined by whole genome sequencing (WGS). The trend of resistance over 20 years in mestizo isolates showed significant resistant increase for clarithromycin and levofloxacin to frequencies that banned its clinical use. Resistance in H. pylori isolates of native communities was lower for all antibiotics tested. Phenotypic resistance showed good to moderate correlation with genotypic tests. Genetic methods for characterizing antibiotic resistance require further validation in each population.https://www.frontiersin.org/articles/10.3389/fcimb.2020.539115/fullHelicobacter pyloriantibiotic resistancewhole genome sequencingphenotypegenotypeindigenous communities |
| spellingShingle | Margarita Camorlinga-Ponce Alejandro Gómez-Delgado Emmanuel Aguilar-Zamora Emmanuel Aguilar-Zamora Roberto C. Torres Silvia Giono-Cerezo Antonio Escobar-Ogaz Javier Torres Phenotypic and Genotypic Antibiotic Resistance Patterns in Helicobacter pylori Strains From Ethnically Diverse Population in México Frontiers in Cellular and Infection Microbiology Helicobacter pylori antibiotic resistance whole genome sequencing phenotype genotype indigenous communities |
| title | Phenotypic and Genotypic Antibiotic Resistance Patterns in Helicobacter pylori Strains From Ethnically Diverse Population in México |
| title_full | Phenotypic and Genotypic Antibiotic Resistance Patterns in Helicobacter pylori Strains From Ethnically Diverse Population in México |
| title_fullStr | Phenotypic and Genotypic Antibiotic Resistance Patterns in Helicobacter pylori Strains From Ethnically Diverse Population in México |
| title_full_unstemmed | Phenotypic and Genotypic Antibiotic Resistance Patterns in Helicobacter pylori Strains From Ethnically Diverse Population in México |
| title_short | Phenotypic and Genotypic Antibiotic Resistance Patterns in Helicobacter pylori Strains From Ethnically Diverse Population in México |
| title_sort | phenotypic and genotypic antibiotic resistance patterns in helicobacter pylori strains from ethnically diverse population in mexico |
| topic | Helicobacter pylori antibiotic resistance whole genome sequencing phenotype genotype indigenous communities |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2020.539115/full |
| work_keys_str_mv | AT margaritacamorlingaponce phenotypicandgenotypicantibioticresistancepatternsinhelicobacterpyloristrainsfromethnicallydiversepopulationinmexico AT alejandrogomezdelgado phenotypicandgenotypicantibioticresistancepatternsinhelicobacterpyloristrainsfromethnicallydiversepopulationinmexico AT emmanuelaguilarzamora phenotypicandgenotypicantibioticresistancepatternsinhelicobacterpyloristrainsfromethnicallydiversepopulationinmexico AT emmanuelaguilarzamora phenotypicandgenotypicantibioticresistancepatternsinhelicobacterpyloristrainsfromethnicallydiversepopulationinmexico AT robertoctorres phenotypicandgenotypicantibioticresistancepatternsinhelicobacterpyloristrainsfromethnicallydiversepopulationinmexico AT silviagionocerezo phenotypicandgenotypicantibioticresistancepatternsinhelicobacterpyloristrainsfromethnicallydiversepopulationinmexico AT antonioescobarogaz phenotypicandgenotypicantibioticresistancepatternsinhelicobacterpyloristrainsfromethnicallydiversepopulationinmexico AT javiertorres phenotypicandgenotypicantibioticresistancepatternsinhelicobacterpyloristrainsfromethnicallydiversepopulationinmexico |