Isolation and identification of cellulose demoting symbionts from gut of subterranean termite, Odontotermes obesus
The study was carried out to isolate and identify the symbionts, viz. cellulose demoting bacteria and fungus in termite gut. The experiment was conducted during (February 2013- July 2013) at biotechnology laboratory, Institute of Pesticide Formulation Technology, Gurgaon (Haryana). Termites are woo...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2015-07-01
|
| Series: | The Indian Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/50142 |
| _version_ | 1797742011000815616 |
|---|---|
| author | D R BAJYA DEEPIKA ARYA M RANJITH M C LAKHARAN S K RAZA |
| author_facet | D R BAJYA DEEPIKA ARYA M RANJITH M C LAKHARAN S K RAZA |
| author_sort | D R BAJYA |
| collection | DOAJ |
| description |
The study was carried out to isolate and identify the symbionts, viz. cellulose demoting bacteria and fungus in termite gut. The experiment was conducted during (February 2013- July 2013) at biotechnology laboratory, Institute of Pesticide Formulation Technology, Gurgaon (Haryana). Termites are wood eating insects and are among the most important ligno cellulose- digesting insects and possess a variety of symbiotic microorganisms in their gut. Nutrient agar, potato dextrose and Carboxy Methyl Cellulose (CMC) were used to isolate the dry bacterial strain and fungus. The cellulose is demoted in termite gut by the production of cellulase enzyme which is detected by Congo red stain. Colony morphology and staining technique such as Gram's staining, Congo red staining and oxidase test for bacterial strain gave an idea for the presence of genera Citrobacter and Enterobacter. Aspergillus nidulans has been isolated and identified at division of plant pathology, IARI, New Delhi. These bacteria and fungus were able to assimilate CMC which aid in digestion of cellulose in subterranean termite Odontotermes obesus (Rambur) and this study abetted to understand more about the symbionts associated with digestive mechanism of termites.
|
| first_indexed | 2024-03-12T14:34:53Z |
| format | Article |
| id | doaj.art-080671db100a4f09af82ac65f7f04414 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-03-12T14:34:53Z |
| publishDate | 2015-07-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-080671db100a4f09af82ac65f7f044142023-08-17T09:16:46ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192015-07-0185710.56093/ijas.v85i7.50142Isolation and identification of cellulose demoting symbionts from gut of subterranean termite, Odontotermes obesusD R BAJYA0DEEPIKA ARYA1M RANJITH2M C LAKHARAN3S K RAZA4Officer Incharage, Institute of Pesticides Formulation Technology, Sector 20, Udyog Vihar, Gurgaon, Haryana 122 016M Sc Biotechnology Scholar, Department of Biotechnology, Maharishi Markandeshwar University, Mullana, HaryanaJRF (Ph D Scholar), Institute of Pesticides Formulation Technology, Sector 20, Udyog Vihar, Gurgaon, Haryana 122 016JRF (Ph D Scholar), Division of Bioscience, Institute of Pesticides Formulation Technology, Sector 20, Udyog Vihar, Gurgaon, Haryana 122 016Director, Institute of Pesticides Formulation Technology, Sector 20, Udyog Vihar, Gurgaon, Haryana 122 016 The study was carried out to isolate and identify the symbionts, viz. cellulose demoting bacteria and fungus in termite gut. The experiment was conducted during (February 2013- July 2013) at biotechnology laboratory, Institute of Pesticide Formulation Technology, Gurgaon (Haryana). Termites are wood eating insects and are among the most important ligno cellulose- digesting insects and possess a variety of symbiotic microorganisms in their gut. Nutrient agar, potato dextrose and Carboxy Methyl Cellulose (CMC) were used to isolate the dry bacterial strain and fungus. The cellulose is demoted in termite gut by the production of cellulase enzyme which is detected by Congo red stain. Colony morphology and staining technique such as Gram's staining, Congo red staining and oxidase test for bacterial strain gave an idea for the presence of genera Citrobacter and Enterobacter. Aspergillus nidulans has been isolated and identified at division of plant pathology, IARI, New Delhi. These bacteria and fungus were able to assimilate CMC which aid in digestion of cellulose in subterranean termite Odontotermes obesus (Rambur) and this study abetted to understand more about the symbionts associated with digestive mechanism of termites. https://epubs.icar.org.in/index.php/IJAgS/article/view/50142BacteriaCelluloseFungiIdentificationIsolationOdontotermes obesus |
| spellingShingle | D R BAJYA DEEPIKA ARYA M RANJITH M C LAKHARAN S K RAZA Isolation and identification of cellulose demoting symbionts from gut of subterranean termite, Odontotermes obesus The Indian Journal of Agricultural Sciences Bacteria Cellulose Fungi Identification Isolation Odontotermes obesus |
| title | Isolation and identification of cellulose demoting symbionts from gut of subterranean termite, Odontotermes obesus |
| title_full | Isolation and identification of cellulose demoting symbionts from gut of subterranean termite, Odontotermes obesus |
| title_fullStr | Isolation and identification of cellulose demoting symbionts from gut of subterranean termite, Odontotermes obesus |
| title_full_unstemmed | Isolation and identification of cellulose demoting symbionts from gut of subterranean termite, Odontotermes obesus |
| title_short | Isolation and identification of cellulose demoting symbionts from gut of subterranean termite, Odontotermes obesus |
| title_sort | isolation and identification of cellulose demoting symbionts from gut of subterranean termite odontotermes obesus |
| topic | Bacteria Cellulose Fungi Identification Isolation Odontotermes obesus |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/50142 |
| work_keys_str_mv | AT drbajya isolationandidentificationofcellulosedemotingsymbiontsfromgutofsubterraneantermiteodontotermesobesus AT deepikaarya isolationandidentificationofcellulosedemotingsymbiontsfromgutofsubterraneantermiteodontotermesobesus AT mranjith isolationandidentificationofcellulosedemotingsymbiontsfromgutofsubterraneantermiteodontotermesobesus AT mclakharan isolationandidentificationofcellulosedemotingsymbiontsfromgutofsubterraneantermiteodontotermesobesus AT skraza isolationandidentificationofcellulosedemotingsymbiontsfromgutofsubterraneantermiteodontotermesobesus |