Spontaneous neutrophil activation in HTLV-1 infected patients
Human T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subject...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600010&lng=en&tlng=en |
| _version_ | 1817976940759351296 |
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| author | Jaqueline B. Guerreiro Maria Aurélia F. Porto Silvane B. Santos Lino Lacerda John L. Ho Edgar M. Carvalho |
| author_facet | Jaqueline B. Guerreiro Maria Aurélia F. Porto Silvane B. Santos Lino Lacerda John L. Ho Edgar M. Carvalho |
| author_sort | Jaqueline B. Guerreiro |
| collection | DOAJ |
| description | Human T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subjects and the association between neutrophil activation and IFN-gamma and TNF-alpha levels. Blood was collected from 35 HTLV-1 carriers, from 8 patients with HAM/TSP (HTLV-1- associated myelopathy); 22 healthy individuals were evaluated for spontaneous and lipopolysaccharide (LPS)-stimulated neutrophil activity (reduction of NBT to formazan). The production of IFN-gamma and TNF-alpha by unstimulated mononuclear cells was determined by ELISA. Spontaneous NBT levels, as well as spontaneous IFN-gamma and TNF-alpha production, were significantly higher (p<0.001) in HTLV-1 infected subjects than in healthy individuals. A trend towards a positive correlation was noted, with increasing percentage of NBT positive neutrophils and levels of IFN-gamma. The high IFN-gamma producing HTLV-1 patient group had significantly greater NBT than healthy controls, 43±24% and 17±4.8% respectively (p< 0.001), while no significant difference was observed between healthy controls and the low IFN-gamma-producing HTLV-1 patient group (30±20%). Spontaneous neutrophil activation is another marker of immune perturbation resulting from HTLV-1 infection. In vivo activation of neutrophils observed in HTLV-1 infected subjects is likely to be the same process that causes spontaneous IFN-gamma production, or it may partially result from direct IFN-gamma stimulation. |
| first_indexed | 2024-04-13T22:10:34Z |
| format | Article |
| id | doaj.art-08071426d7294e3d85aa4812bb06f21d |
| institution | Directory Open Access Journal |
| issn | 1678-4391 |
| language | English |
| last_indexed | 2024-04-13T22:10:34Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-08071426d7294e3d85aa4812bb06f21d2022-12-22T02:27:45ZengElsevierBrazilian Journal of Infectious Diseases1678-43919651051410.1590/S1413-86702005000600010S1413-86702005000600010Spontaneous neutrophil activation in HTLV-1 infected patientsJaqueline B. Guerreiro0Maria Aurélia F. Porto1Silvane B. Santos2Lino Lacerda3John L. Ho4Edgar M. Carvalho5Universidade Federal da BahiaUniversidade Federal da BahiaUniversidade Federal da BahiaUniversidade Federal da BahiaCornell UniversityUniversidade Federal da BahiaHuman T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subjects and the association between neutrophil activation and IFN-gamma and TNF-alpha levels. Blood was collected from 35 HTLV-1 carriers, from 8 patients with HAM/TSP (HTLV-1- associated myelopathy); 22 healthy individuals were evaluated for spontaneous and lipopolysaccharide (LPS)-stimulated neutrophil activity (reduction of NBT to formazan). The production of IFN-gamma and TNF-alpha by unstimulated mononuclear cells was determined by ELISA. Spontaneous NBT levels, as well as spontaneous IFN-gamma and TNF-alpha production, were significantly higher (p<0.001) in HTLV-1 infected subjects than in healthy individuals. A trend towards a positive correlation was noted, with increasing percentage of NBT positive neutrophils and levels of IFN-gamma. The high IFN-gamma producing HTLV-1 patient group had significantly greater NBT than healthy controls, 43±24% and 17±4.8% respectively (p< 0.001), while no significant difference was observed between healthy controls and the low IFN-gamma-producing HTLV-1 patient group (30±20%). Spontaneous neutrophil activation is another marker of immune perturbation resulting from HTLV-1 infection. In vivo activation of neutrophils observed in HTLV-1 infected subjects is likely to be the same process that causes spontaneous IFN-gamma production, or it may partially result from direct IFN-gamma stimulation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600010&lng=en&tlng=enHTLV-1IFN-gammaTNF-alphaLPSneutrophil activation |
| spellingShingle | Jaqueline B. Guerreiro Maria Aurélia F. Porto Silvane B. Santos Lino Lacerda John L. Ho Edgar M. Carvalho Spontaneous neutrophil activation in HTLV-1 infected patients Brazilian Journal of Infectious Diseases HTLV-1 IFN-gamma TNF-alpha LPS neutrophil activation |
| title | Spontaneous neutrophil activation in HTLV-1 infected patients |
| title_full | Spontaneous neutrophil activation in HTLV-1 infected patients |
| title_fullStr | Spontaneous neutrophil activation in HTLV-1 infected patients |
| title_full_unstemmed | Spontaneous neutrophil activation in HTLV-1 infected patients |
| title_short | Spontaneous neutrophil activation in HTLV-1 infected patients |
| title_sort | spontaneous neutrophil activation in htlv 1 infected patients |
| topic | HTLV-1 IFN-gamma TNF-alpha LPS neutrophil activation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600010&lng=en&tlng=en |
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